This article discusses 4 methods of deepening reformation entirely to improve quality of running a school in medical college in comprehensive university,such as how to enhance framework adjustment of specialty and construct scientific and reasonable framework of specialty,how to strengthen basic foundation of teaching,improve condition of running a school,how to deepen teaching reformation and reform pattern of students'cultivation,and how to strengthen teaching management and establish system of quality control.All these methods were detailed in this article to offer certain practical experience for other similar medical colleges in comprehensive universities.

Project and tutor system applied in experimental teaching is one important teaching reform project,which achieved significant teaching effect. The paper analyzes the necessity of project and tutor system,implies compiling teaching materials,training for teaching staff and improvement of experimental conditions,and values effective implementation and the summarization and perfection of teaching work.

Objective To observe the migration and distribution of OECs in injured spinal cord and discuss their relation with the recovery of spinal cord function. Methods The rats were contused by a force of 10 g · 25 mm with NYU-impactor at T10 level. The OECs acutely isolated from green fluorescence protein (GFP) rats were purified, identified and then transplanted into the injured site and the rostral and caudal parts of the spinal cord one week after injury, with total volume of the transplanted OECs for 90 000/μl. Within 13 weeks after transplantation, the migration and distribution of OECs were qualitatively observed on the cryo-sections under fluorescence light microscope. The area and the length of OECs distribution were semi-quantitatively determined. The locomotor function of the spinal cord was appraised by BBB score. Results OECs were located collectively in the transplanted site at early stage after transplantation and then spread gradually mainly along the long axis of the cord. OECs could be found in the cavity of the contused spinal cord. The area and the length of OECs distribution were increased from 1.33 mm2 and 4.23 mm respectively at one week to 3.30 mm2 and 7.68 mm respectively at 13 weeks after transplantation. In the meantime, the locomotor function was gradually improved. Conclusion OECs can migrate within the contused spinal cord, as may contribute to the recovery of locomotor function.

Objective To observe the expression of brain-derived neurotrophical factor (BDNF) in injury spinal cord after transplantation olfactory ensheathing cells (OECs), and to investigate the mechanism of OECs repairing spinal cord injury.Methods OECs from GFP transgenic rats were separated and cultured for transplantation. Spinal cord injury rats were separated two groups by random digits table. In experimental group, OECs suspension were transplanted into injured spinal cord following spinal cord injury. In control group, DMEM was transplanted into the injured spinal cord after spinal cord injury. Motor function was evaluated per week after transplantation. The expression levels of BDNF mRNA and protein were detected by using RT-PCR and immunohistochemistry respectively, and compared with those from normal SD rats.Results Motor function of two groups was improved gradually after transplantation. The motor function scores in experimental group was obviously higher than in control group at 21st day after transplantation (P<0.05). A lot of survival GFP OECs distributed around impaired myeloid tissue. At 21st day after transplantation, BDNF mRNA and protein expression in experimental group were strongest (P<0.05), and stronger in control group than in normal group (P<0.05).Conclusion The transplantation of OECs can repair the injured spinal cord by increasing the expression of BDNF mRNA and protein to improve local microenvironment.

Objective To determine the biological effectiveness of 125I radioactive seeds with continuous low dose rate radiation on the human esophageal cancer cell line KYSE150 in vitro and explore the underlying cellular mechanisms.Methods The cells were divided into three cell groups:control group,single dose radiation group (SDR) and 125I radioactive seeds with continuous low dose rate radiation group (125 I-CLDR).The KYSE150 cells were exposed to radiation of X-ray at a high dose rate of 1.052 Gy/min or 125I radioactive seeds at a low dose rate of 2.77 cGy/h.The responses of KYSE150 cells to two modes of irradiation were evaluated by the colony-forming assay,cell apoptosis as well as cell cycle analysis.Furthermore,the expression levels of γ-H2AX and Bax were detected by Western blot.Results KYSE150 cells were more radiosensitive to 125I-CLDR than SDR.The relative biological effectiveness (RBE) for 125I-CLDR related to SDR was 1.56.Compared with SDR,125I-CLDR yielded more proportions of the early and late apoptosis rate (t =4.07,11.08,P ＜0.05) as well as cells at G2/M phase (t =11.25,P ＜0.05).Moreover,γ-H2AX and Bax expression levels in 125I-CLDR significantly increased compared with SDR.Conclusions Compared with the high dose rate X-ray radiation,the continuous low dose rate radiation of 125I radioactive seeds had stronger inhibition effect on KYSE150 esophageal cancer cells by impairing clonogenic capacity,inducing apoptosis and G2/M cell cycle arrest,and increasing radiosensitivity.

Objective To explore the anti-HBV activity of anodonta polysaccharides (AP) and dose-effect relationship in vitro.Methods HepG2.2.15 cells were cultured in vitro and incubated at 37℃ for nine days with AP at a dilution ratio of 1∶10.The expression of HBsAg and HBeAg were detected using ELISA and HBV-DNA copies were detected by real-time fluorescent quantitative PCR.Based on Thiele-type continued-fraction interpolation method,the anti-HBV activity of AP was studied,and the IC50 and the maximum inhibition rate were calculated.Results AP had significant inhibitory effect on the expression of HBsAg and HBeAg in HepG2.2.15 cells in vitro,as well as HBV DNA replication.By Thiele-type continued-fraction interpolation the equations of dose-effect relationship were obtained to determine the maximum inhibition rates of AP on HBeAg and HBsAg secretion being 47.7％ and 56.4％,and the IC50 inhibiting the expression of HBeAg being 143.7mg/L.AP was also able to inhibit HBV-DNA replication and the maximum inhibition rate was 17.8％ with the same method above.Conclusion Anodonta polysaccharides have anti-HBV activity.The thiele-type continued-fraction interpolation method is simple and practical and could be used as a new method for the analysis of drug activity.

Ten dogs were divided into 2 groups and absolute ethanol was injected into the renal artery and its branches respectively. The kidneys injected were resected by stages and examined pathologically. The results showed that coagulative necrosis developed in the renal artery and kidney itself. In the group whose branches of the renal artery were injected there was a clear demarcation line between the necro-tic and the normal areas. The thrombus formed secondary to necrosis might worsen the blockage of the renal artery. The clinical observations on the renal artery injected with absolute ethanol in 15 cases showed that the method of absolute ethanol injection was better than any other methods or drugs for the renal artery. The injection was safe, convenient and more favourable to the prevention of the spread of cancer cells.

Objective To evaluate the safety and effectiveness of enhanced recovery after surgery (ERAS) in laparoscopic radical gastrectomy for gastric cancer.Methods From May 2015 to July 2017,149 patients with gastric cancer in our department were prospectively enrolled and randomly divided into the ERAS group (n =75) and control group (n =74).Results In ERAS group compared to control group,the time to first passage of flatus was (51 ± 11)vs.(62 ± 11)h,first feeding time (46 ± 12) vs.(68 ±20)h,gastric tube removal time (13 ± 12)h vs.(70 ± 16) h,pain score on the first day after surgery (3.9 ±1.3) vs.(5.2 ±0.9),C-reaction protein level (8.5 ±2.6) mg/L vs.(10.1 ±3.0)mg/L,post-op hospital stay (6.9 ± 2.9) d vs.(11.2 ± 3.5) d,were all significantly different (all P ＜ 0.05).The postoperative complication rates was 25％ vs.28％ respectively,(x2 =0.101,P =0.750).Conclusions Enhanced recovery after surgery can promote the postoperative recovery and shorten the time of hospitalization in laparoscopic-assisted radical gastrectomy for gastric cancer.