Objective To investigate the expression of hypoxia-inducible factor-1α (HIF-1α),insulin-like growth factor (IGF-1) and epidermal growth factor receptor (EGFR) in non-small cell lung cancer (NSCLC) and their clinical significance.Methods The expression of HIF-1α,EGFR and IGF-1 in NSCLC tissues and adjacent non-tumor tissues were detected by western blot,and the correlation with clinicopathological features and prognosis were analyzed.Results The expression of HIF-1α,IGF-1 and EGFR in NSCLC tissues were significantly higher than that in non-tumorous tissues.There were no significant difference in the expression of HIF-1 α and IGF-1 in squamous cell carcinoma and adenocarcinoma(P ＞ 0.05).The expression of EGFR in squamous cell carcinoma was significantly higher than that in adenocarcinoma(P ＜ 0.05).The expression of HIF-1α,IGF-1 and EGFR in low differentiated carcinoma were significantly higher than those in medium and high differentiated carcinoma tissues,and the expression of HIF-1α,IGF-1 and EGFR in stage Ⅰ and Ⅱ tumor tissues were significantly lower than those in stage Ⅲ and Ⅳ tumor tissues(P ＜0.05).The expression of HIF-1 α,IGF-1 and EGFR with lymph node metastasis were significantly higher than that without lymph node metastasis in tumor tissues.There was a significant positive correlation between the expression of HIF-1 α and EGFR (r2 =0.301,P ＜ 0.05),and the expression of HIF-1 α was positively correlated with the expression of IGF-1 (r2 =0.611,P ＜ 0.05).The expression of HIF-1α,EGFR and IGF-1 had no significant correlation with the prognosis (P ＞ 0.05).Conclusion HIF-1,EGFR and IGF-1 were significantly increased in NSCLC tissues,and HIF-1 was positively correlated with EGFR and EGF-1,and the expression of HIF-1α,IGF-1 and EGFR are closely related to the degree of differentiation,TNM staging and lymph node metastasis,and EGFR is closely related to pathological type.They play an important role in the development and progression of NSCLC.