Materials: leaves and flowers of Stephanie glabra (Roxb) collected from Ninh Binh and Ha Noi (sample plants) through 3 flowering seasons. Methods: observations by steric microscopy (Nikon-Japan). The botanical features of Stephanie glabra: creeping stem, leaf peduncle (5-10cm), leaf nervure (11), masculine inflorescence, inflorescence pedicle has 5-6 umbels, each umbel has 8 secondary umbels, each secondary umbel has 4 third umbels, the inflorescence ended by 3 flowers with short pedicles. Masculine flower has 6 separate calixes, which were arranged as 2 circles. Ovular calix. Ovular tepal (3), gamo staminate with 6 slight yellow microsporangiums. The microsporangium changed into pill.
anatomy & histology ; anatomy & histology ; Plant Leaves

Objective: To establish a surveillance in Dong Thap, at the border with Cambodia by assessing the presence of DENV serotypes and CHIKV among patients hospitalized at Dong Thap general hospital. Methods: Cross-sectional descriptive analysis was conducted on a cohort of 131 patients hospitalized with acute fever and symptoms compatible with dengue or chikungunya. The study was conducted from January 2012 to February 2013. The full clinical picture was established as well as serological and molecular detection. Serological analysis was sequentially performed on blood samples collected on admission and an average of seven days after admission. The detection of IgM antibody to DENV was performed by IgM capture ELISA and the detection of DENV and CHIKV RNA was done by reverse-transcription multiplex PCR. Results: 101 patients out of 131 (77%) were confirmed with dengue. All four dengue serotypes were detected with a predominance of DENV2 and DENV4. No chikungunya infection was detected although reported in neighboring Cambodia. A differential efficiency of serological dengue detection was observed. Efficiency was 29% upon admission and 53% after seven days on the same patients. 30 patients out of 131 (23%) were negative with both DENV and CHIKV. Conclusions: Dengue is at risk of being underestimated and chikungunya is not systematically detected. Changes in detection and surveillance procedures are therefore discussed to increase efficiency of dengue detection and continue the monitoring the emergence of CHIKV in Dong Thap province and in Vietnam.