The rat anti-HRP McAbs were prepared with rat-rat hybridoma and the rat McPAP complex was made by conjugating this McAb with HRP. The results of the present work indicated that the rat MePAP can be used for detection of antibodyantigen reaction by immunohistochemical techniques and ELISA. In both assay it gave strong staining reaction for positive antigen and with background staining appeared very pale. The sensitivity of rat McPAP and HRP labelling rabbit anti-rat IgG has been compared.

Objective:To investigate the effects of brain asymmetry on levels of IL-1?,IL-6 and suppressor of cytokine signaling-3(SOCS-3) in hypothalamus,to get better understanding of relationship between brain asymmetry and neuro-immuno-endocrine network at the molecular level.Methods:By using paw preference test,right-pawed,left-pawed,and ambidextrous mice model was established.After decapitation of the mice,hypothalamus was separated rapidly.Some samples were homogenized and used for detection of levels of interleukin-1?(IL-1?),interleukin-6(IL-6) by Enzyme-Linked-Immunosorbent Assay(ELISA).The other samples were applied to detect SOCS-3 expression level by RT-PCR.Results:(1)The level of IL-6 in hypothalamus:left-pawed were higher than right-pawed mice,there was significant difference in statistics( P 0.05).(3)The expression level of SOCS-3:the SOCS-3 expression of right-pawed showed higher than left-pawed mice in hypothalamus,revealing a significant difference( P

AIM: To investigate the influence of predator stress on hypothalamic-pituitary-adrenal (HPA) axis and IL-1?, IL-6 in mice with brain asymmetry. METHODS: By using paw preference test, right-pawed, left-pawed, and ambidextrous-pawed mice model were established. Mice with brain lateralization were exposed to their predator (cat). After acute and chronic predator stress by cats, EIA and ELISA were applied to detect plasma levels of corticosterone (CS), IL-1? and IL-6. RESULTS: (1) The level of plasma CS: in both acute and chronic predator stress group, right-pawed and ambidextrous mice had a higher level than that in their corresponding normal group (P

Objective To study the vascular endothelial growth factor(VEGF) expression and secretion of mesenchymal stem cells(MSCs) of rabbit transfected with pcDNA3-VEGF165 expression plasmid in order to construct a kind of tissue engineering artificial skull with more blood supply.Methods By gene reconstruction method,the VEGF165 gene was cloned into eukaryotic expression plasmid pcDNA3 and recombined eukaryotic expression plasmid pcDNA3-VEGF165 was constructed;By lipofectamine transfection method,pcDNA3-VEGF165 expression plasmid was transfected into MSCs of rabbit;By RT-PCR and Western blotting methods,the VEGF mRNA expression and VEGF protein secretion in the MSCs were detected.Results Recombined eukaryotic expression plasmid pcDNA3-VEGF165 was confirmed to be true by double enzyme digestion,two strips came to appear in 5400 bp and 600 bp of gelose electrophoresis and their sizes accorded with pcDNA3 plasmid and VEGF165 accordingly;Primarily cultured and subcultured MSCs of rabbit were successfully performed and the MSCs storehouse of rabbit was established,primary MSCs presented lymphoid form at first and then the morphology of them became circulara,polygonal or irregular forms,they were more and more like fibroblastic cells after subcultured cultivation.The expressions of VEGF mRNA and VEGF protein in the MSCs were found after transiently transfection by RT-PCR and Western blotting methods.Conclusion Recombined eukaryotic expression plasmid pcDNA3VEGF165 can be transfected into MSCs of rabbit effectively by lipofectamine and the VEGF expression can be detected in the MSCs after transfection.

Objective The incidence rate of complication during central venous catheterization through internal jugular vein (IJV) and subclavian vein (SV) puncture. Methods Clinical controlled trials about IJV and SV puncture were collected and related literatures were screened according to the criteria of inclusion. The literatures underwent Meta analysis and subsequent analysis of sensitivity. Results A total of 18 literatures were included. Meta analysis indicated that statistical difference existed in the related infection rate [RR=1.74, 95%CI (1.32, 2.30)] and arterial puncture [RR=3.19, 95%CI (1.70, 5.99)], but not in the one-time-puncture success rate [RR=1.06, 95%CI (0.90, 1.24)] between IJV and SV puncture. Conclusions The rate of related infection and arterial puncture was higher by IJV than by SV puncture. But we could not confirm if any difference existed in the one-time-puncture success rate between the two methods. The results still needs evaluation by high-quality randomly controlled experiments.

Purpose　The aim is to invest the mechanism and activity of kringle1～3 gene of angiostatin. Methods　Kringle1～3 gene of angiostatin was abtained from hepatocyte of normal human by RT-PCR and inserted into expression vector pPIC9K in Pichia expression system. The recombinant protein was induced secrete , purified and test the activity by MTT.Results　　Krigle1～3 protein could inhibit vascular endothelial cell proliferative activity.Conclusion　The reaction similar apoptosis induced by kringle1～3 recombinant protein of angiostatin inhibit cell proliferation.

Objectives To analyze the relationship between dietary carbohydrate and glycemic load(GL) and the plasma lipid level and dyslipidemia risk. Method The sample of adults above 18y of age from "National Survey on Nutrition and Health Status of the Chinese People 2002" was used,excluding pregnant women,mothers and people who controled their diets. Data of GI from China Food Composition 2002 and International Glycemic Index Table 2002 were used. The dietary GL as the indicator of dietary carbohydrate was calculated by multiplying the carbohydrate content of each food by its glycemic index,then multiplied by frequency of consumption and summed up from all food. Correlation analysis,multiple regression and Logistic regression were used to analyze the relationship between dietary GL and plasma lipid level and dyslipidemia risk. Results Dietary GL remained inversely associated with plasma total cholesterol(TC) and low density lipoprotein cholesterol(LDL-C) . With the increase of dietary GL,the risk of hypercholesterolemia and high blood LDL-C decreased. In the meantime dietary GL remained negatively associated with plasma high density lipoprotein cholesterol(HDL-C) . With the increase of dietary GL,the risk of low blood HDL-C increased. Dietary GL remained inversely associated with plasma triglyceride(TG) ,but showed no significant influence on risk of hypertriglyceridemia. Conclusion The traditional Chinese dietary pattern which is characterized by cereals as staple food is helpful to decrease dyslipidemia risk.

In order to observe the effect of the immune and weight of chickens after use the attenuated vaccine with low dose of chicken infectious anemia virus (CIAV). In this study, the effects of low dose of CIAV on the weight of SPF chickens and NDV antibody production were observed by simulated experiments. The results showed that 10 EID50 and 5 EID50 CIAV per plume attenuated NDV vaccines were used to cause the weight loss of SPF chickens. Compared with the use of the non contaminated vaccine group, it has significant difference. And NDV antibody levels compared with the use of the non contaminated groups also decreased after use the vaccine with two doses of CIAV contaminated. It has significant difference. A certain proportion of CIAV antibody positive was detected at the beginning of the second week after use the NDV vaccine with two doses of CIAV contaminated. The detection of a high proportion of CIAV nucleic acid was detected in the first week after the use of a contaminated vaccine. The results of the study demonstrate the effects of CIAV pollution on the production and immune function of SPF chickens, and it is suggested that increasing the detection of viral nucleic acid can help save time and improve the detection rate in the detection of exogenous virus contamination by SPF chicken test method.
Animals ; Antibodies, Viral ; immunology ; Chicken anemia virus ; genetics ; immunology ; physiology ; Chickens ; Circoviridae Infections ; immunology ; veterinary ; virology ; Poultry Diseases ; immunology ; virology ; Specific Pathogen-Free Organisms ; Vaccines, Attenuated ; administration & dosage ; genetics ; immunology

Objective To understand the relationship between the dietary patterns and metabolic syndrome among elderly people in Zhejiang province,in order to provide scientific basis and reference for nutrition interventions.Methods Data were obtained from the 2010-2012 Chinese National Nutrition and Health Survey,and factor analysis was used to obtain food patterns among elderly people in Zhejiang province.The Logistic regression was used to explore the association of the food patterns with metabolic syndrome and its components.Results The intakes of bean products,nuts,fruits,eggs,and milk in elderly people were generally low,and the intakes of livestock and poultry meat,cooking oil and salt were relatively high.5 dietary patterns were derived by factor analysis:high-quality protein diet,mediterranean diet,condiments diet,traditional dietary pattern and western dietary pattern.The medical test results in 780 elderly people showed that the standardized prevalence of metabolic syndrome in the elderly was 28.8％,standardized central obesity rate was 41.2％,standardized hypertension rate was 50.3％ and standardized hyperglycemia rate was 39.5％.Logistic regression analysis showed that different dietary patterns had different effects on the metabolic syndrome and its components.Conclusions The prevalence rate of metabolic syndrome and the abnormal rates of its components are high in the elderly in Zhejiang.The intakes of fruits and milk are generally low,and intakes of livestock and poultry meat,cooking oil and salt are relatively high.The relationship between dietary patterns and MS or its components is complicated,so it is necessary to guide the elderly people to adopt reasonable diet by referring to the Diet Pagoda in order to reduce the risk of MS or its components.

Objective To evaluate the effect and side effects of Kezhi capsule in short-term treatment of nonalcoholic fatty liver disease (NAFLD). Methods 60 NAFLD cases of outpatients according to the inclusion criteria of were randomly assigned to two groups: the treated group-30cases with the prescription of Kezhi capsule , and the control group-30cases with the prescription of Xuezhikang. The treatment Course of taking Kezhi capusule (1.25 g, tid, po) and Xuezhikang(0.6 g, bid, po)was 24 weeks. Results After treatment in both groups we saw the significant decrease of the levels of ALT, AST, TC, TG, LDL-C, CREAT, BUN, BMI and TCM Syndromes scores, and the improvement of the ultrasonographic findings of liver steatosis. In the Xuezhikang group we saw higher decrease of TC and TG than the Kezhi capsule group with statistic difference (P < 0.05), while in Kezhi capsule group we saw higher decrease of BMI , TCM Syndromes scores and the improvement of the ultrasonographic findings of liver steatosis than the xuezhikang group with statistic difference (P < 0.05). Conclusions The results show that kezhi capsule is effective for the treatment of NAFLD without obvious side effects.