Objective To establish a rapid,accurate,specific quantitative assay for detecting B.pertussis,and apply to clinical diagnosis.Methods According to the specific sequence of B.pertussis IS481 gene,the primers and the fluorescence probe were designed and synthesized.Then a fluorescence quantitative PCR for detecting B.pertussis was developed.The specificity,sensitivity and reproducibility of the method were evaluated.255 specimens including 225 nasopharyngeal swabs from suspected pertussis patients and 30 normal nasopharyngeal swabs were detected by fluorescence quantitative PCR.Results A rapid specific quantitative method for detecting B.pertussis was established.The standard curve of the method indicated that there was a good linear relationship between the CT value and the template concentration with the correlation coefficient being 0.998.The linear range of the system was from 102 to 108 copies/μl and the minimum was 102 copies.It had a high sensitivity and good specificity.The intra.and inter-assay coefficients of variation were 5.78%-16.7% and 8.25%-14.9% respectively.The fluorescence quantitative PCR identified 41 positive results for specimens from suspected pertussis patients and results of 30 normal specimens were all negative.Conclusions The method can quantitatively detect the B.pertussis rapidly with high sensitivity and specificity,it can be applied to clinical diagnosis.

Objective:To investigate the status of dyadic coping among patients with lung cancer and their spouses, and explore the related factors of dyadic coping.Methods:A convenience sample of 227 patients with lung cancer and their spouses were selected. The measurements of socio-demographic questionnaire, Dyadic Coping inventory (DCI), the Quality of Relationship index (QRI) and Connor-Davidson Resilience Scale (CD-RISC-10) were used to assess the general information, dyadic coping, relationship satisfaction and resilience, respectively.Results:The DCI score of patients were (3.13±0.89) points; the DCI score of spouses were (3.03±0.93) points; the scores of DCI were significant correlated between patients and their spouses ( r values were 0.248-0.632, P<0.01). The relationship satisfaction ( β values were 0.421, 0.474, P<0.01) and resilience ( β values were 0.374, 0.211, P<0.01) were the significant predictor of dyadic coping. Meanwhile, partner effects for patients′ resilience ( β value was 0.193, P<0.05) and spouse′ relationship satisfaction ( β value was 0.237, P<0.01) were also significant. Conclusions:Moderate dyadic coping were occurred among patients with lung cancer and their spouses. Relationship satisfaction and resilience were the mainly predictors for dyadic coping. To improve relationship satisfaction and enhance resilience might be an effective approach to promote positively dyadic coping.