Objective To explore the application of change speed of lithotomy position in the patients with gynecological laparoscopic surgery.Methods A total of 120 gynecological laparoscopic surgery patients in our hospital were randomly divided into observation group and control group,pneumoperitoneum pressure of two groups was 11 mmHg.The change speed of lithotomy position in the control group was in 6 s,the observation group was ＞ 30 s,and the changes of hemodynamics and blood gas indexes were compared.Results SBP and DBP after pneumoperitoneum in the two groups were increased compared with that before pneumoperitoneum (P ＜ 0.05).The increase of DBP and SBP after pneumoperitoneum in the observation group was less than that in the control group (P ＜ 0.05).PETCO2 after pneumoperitoneum in the observation group showed no statistically significant compared with pneumoperitoneum before (P ＞ 0.05),but the control group had higher PETCO2 than that before pneumoperitoneum,there were statistically significant differences (P ＜ 0.05).The observation group and the control group had a tendency to rise in airway pressure after pneumoperitoneum,but no statistical significance between the two groups was found (P ＞ 0.05).Conclusion Prolonging change time of lithotomy position can reduce the influence of hemodynamics for the patients with gynecological laparoscopic operation,and it is beneficial to the recovery of lung function.

Objective To explore the application of change speed of lithotomy position in the patients with gynecological laparoscopic surgery.Methods A total of 120 gynecological laparoscopic surgery patients in our hospital were randomly divided into observation group and control group,pneumoperitoneum pressure of two groups was 11 mmHg.The change speed of lithotomy position in the control group was in 6 s,the observation group was ＞ 30 s,and the changes of hemodynamics and blood gas indexes were compared.Results SBP and DBP after pneumoperitoneum in the two groups were increased compared with that before pneumoperitoneum (P ＜ 0.05).The increase of DBP and SBP after pneumoperitoneum in the observation group was less than that in the control group (P ＜ 0.05).PETCO2 after pneumoperitoneum in the observation group showed no statistically significant compared with pneumoperitoneum before (P ＞ 0.05),but the control group had higher PETCO2 than that before pneumoperitoneum,there were statistically significant differences (P ＜ 0.05).The observation group and the control group had a tendency to rise in airway pressure after pneumoperitoneum,but no statistical significance between the two groups was found (P ＞ 0.05).Conclusion Prolonging change time of lithotomy position can reduce the influence of hemodynamics for the patients with gynecological laparoscopic operation,and it is beneficial to the recovery of lung function.

Objective To enhance residents′tuberculosis awareness rate of prevention and treatment in Guangxi, and provide evidence for health education and citizen′s awareness of tuberculosis prevention. Methods A total of 735 young urban and rural residents in Guangxi were investigated by awareness rate of tuberculosis prevention and control knowledge questionnaire.Results Tuberculosis disease awareness rate was 96.2%(707/735) in Guangxi,that of 78.6% (556/707) residents had the awareness rate of tuberculosis spread, 57.4%(406/707) knew policy for free tuberculosis treatment and 43.3%(306/707) of residents understood tuberculosis could heal. The rural group awareness rate was lower than county residents for tuberculosis spread and free treatment policy (χ2 =4.992,5.786, respectively;P<0.05).Conclusions The awareness rate of tuberculosis′prevention and treatment knowledge is lower than the national average, so it should strengthen the prevention and propaganda of tuberculosis.

OBJECTIVE: To establish a non-invasive method for beta-thalassemia by detecting parental CD41-42 mutation in cell-free DNA derived from maternal plasma with droplet digital PCR (ddPCR). METHODS: Beta-actin gene and beta-thalassemia gene CD41-42 mutation were respectively set as the reference and target sequences. A novel method was established based on Bio-Rad ddPCR technique with specific primers and TaqMan probes for the two genes. The accuracy, sensitivity and detective linearity range of the developed method were evaluated by detection of the target gene gradient concentration samples. The applicability was also evaluated by testing 20 maternal plasma samples. RESULTS: The ddPCR method could accurately detect the beta-thalassemia CD41-42 mutation in cell-free DNA derived from maternal plasma. Within the target sequence concentration ratio of 5.00%-0.50%, the relative errors were all < 0.05, the linear regression equation was Y=1.0101-X-0.0071 and R=0.9994. The results of 20 maternal plasma cell-free DNA samples were all consistent with those of the follow-up testing. CONCLUSION A ddPCR method for detecting parental CD41-42 mutation in cell-free DNA from maternal plasma was developed. The method is simple, rapid, accurate, and can be applied for non-invasive prenatal diagnosis for couples simultaneously carrying the CD41-42 mutation.
Cell-Free Nucleic Acids ; DNA ; blood ; Female ; Humans ; Mutation ; Polymerase Chain Reaction ; Pregnancy ; Prenatal Diagnosis ; methods ; beta-Thalassemia ; diagnosis ; genetics

Objective To explore the effect of oropharyngeal healthy exercise combined with guided education in the treatment of dysphagia in patients with Alzheimer's disease (AD). Methods 90 AD inpatients in the geriatric psychiatric department of Guangzhou Huiai Hospital during May 2014 and August 2015 were equally randomized into the study group and the control group with a random digit table:the former group was treated with routine oral muscles training and the latter with oropharyngeal healthy exercises combined with guided education plus routine muscles training. The comparison was done between the groups in terms of the total curative effectiveness 4 weeks following the intervention. Result The total effective rate in the study group was significantly higher than that in the control group (P<0.01). Conclusion The oropharyngeal healthy exercise combined with guided education can effectively promote the recovery of swallowing function in AD patients.

Tripterygium wilfordii is a valuable medicinal plant rich in biologically active diterpenoids, but there are few studies on the origins of these diterpenoids in its secondary metabolism. Here, we identified three regions containing tandemly duplicated diterpene synthase genes on chromosomes (Chr) 17 and 21 of T. wilfordii and obtained 11 diterpene synthases with different functions. We further revealed that these diterpene synthases underwent duplication and rearrangement at approximately 2.3-23.7 million years ago (MYA) by whole-genome triplication (WGT), transposon mediation, and tandem duplication, followed by functional divergence. We first demonstrated that four key amino acids in the sequences of TwCPS3, TwCPS5, and TwCPS6 were altered during evolution, leading to their functional divergence and the formation of diterpene secondary metabolites. Then, we demonstrated that the functional divergence of three TwKSLs was driven by mutations in two key amino acids. Finally, we discovered the mechanisms of evolution and pseudogenization of miltiradiene synthases in T. wilfordii and elucidated that the new function in TwMS1/2 from the terpene synthase (TPS)-b subfamily was caused by progressive changes in multiple amino acids after the WGT event. Our results provide key evidence for the formation of diverse diterpenoids during the evolution of secondary metabolites in T. wilfordii.