ObjectiveTo study the risk factors of spontaneous bacterial peritonitis(SBP) in cirrhotic patients with ascites. MethodsThe clinical data of 186 cirrhotic patients with ascities from February 2005 to March 2010 were retrospectively analyzed were divided into two groups,the one with SBP and the other without SBP. The following factors such as sex, Child-Pugh grade were analyzed with binary logistic regression. ResultsAmong those factors ,3 factors were proved to be risk factors for SBP in cirrhotic patients with ascites by analysis of single and multiple variables. Those were Child-Pugh grade score, serum bilirubin level and ascitic fluid albumin. ConclusionSBP in cirrhotic patients with ascites was affected by many factors. The Child-Pugh grade score, serum bilirubin level and ascitic fluid albumin were independently associated with risk factors of SBP, so it was important to pay attention to those factors in preventing the onset of SBP.

Young Kunming mice were fed with the food containing mercuric chloride, lithium carbonate, lead acetate or cadmium chloride for 45 days. It was found that these 4 salts of metals could all result in different degrees of functional and structural damages of the kidneys of the mice. In the mercuric chloride and lead acetate groups. the liver and the duodenum were also severely damaged besides the renal damages and the mice suffered a high mortality during the period of taking the toxicants. In the lithium carbonate group, the mortality was also very high but the histological damages of the kidneys were less severe. In the cadmium chloride group, the renal damages were more severe than in the other 3 groups, the mortality of the mice was relatively low, the histology of the heart, the liver and the duodenum was essentially normal, and the renal damages became worse even the toxicant was stopped. It is concluded that cadmium chloride is the agent of choice to establish the mouse model of chronic renal failure.

AIM: To explore transdifferentiation potential of Sca-1 + cells from murine fetal liver. METHODS: 2?10 3 of Sca-1 + cells from male murine fetal liver were transfused into female mouse irradiated lethally with ? ray from 60 Co source (10 Gy) via tail vein. Two months later, FISH and immunohistochemistry were used to detect the situation for transdifferentiating of the donor cells (male cells) in tissues of female recipient mouse. RESULTS: The renal tubular epitheliocyte-like and neurocyte-like cells with Y chromosome were found on the sections of renal and brain tissues from female recipient mice. These cells have phenotype characteristics of RCA+/CD - 45 F - 4/80 and NueN +/CD -45 F - 4/80, respectively. CONCLUSION: The evidence is provided for Sca-1 + cells from murine fetal liver to transdifferentiate into both renal and brain tissue cells.

Pancreatic islet β-cell mass is regulated by β-cell replication, nengenesis, apnptosis and cell size. β-cetl mass in diahetic patients is conspicuously less than that in normal subjects. Induction of β-cell regeneration and inhibition of β-cell apoptosis is the new target of diabetes treatment.

Construction of research-based university of Traditional Chinese Medicine is an important orientation of running our university,and more importantly,is the strategy of developing our university.This article analyses the strategies of building a research-based University of Traditional Chinese Medicine form the idea of running schools,characteristics of development,deepening reform,and other perspectives.

Four fresh corpses of infant are used in this research. Three-dimensional microvasculatures of the sinoatrial node and atrioventricular node were studied by means of the observation of vascular casts with SEM. The results showed that the vascular bed of the sinoatrial node was consisted of the microvascular networks. It was oval in shape. The central artery penetrated this node, through its longitudinal axis and divided progressively into arterioles and precapillary arterioles and finally, they branched into capillary networks at the superficial part of the node. The postcapillary venules were characterized by draining blood in accordance with area. The microvascular construction of atrioventricular node was an oblate microvascular network in shape. There was a layer of delicate capillary networks at its superficial part. In the depth of the capillary networks, the venous plexus which was thick and sinusoidal in shape was observed through the meshes. The artery of atrioventricular node entered the node from one side of it. In the node, the artery ramified by degrees to periphery into precapillary arterioles, which penetrated venous plexus and connected with capillary networks in the superficial part of the node. There were evidently narrow rings, whick were impression of the muscle sphincters at the origin of thep ostcapillary venules.

Objective To explore the effect of let-7c-1 on the learning and memory of PTZ-induced epileptic rats and its relevant mechanism.Methods A model of temporal lobe epilepsy (TLE) was induced via PTZ kindling in SD male rats.The epileptic rats were divided into epilepsy group,agomir-control group,let-7c-1 agomir group (12 rats for each).Twelve rats were served as a negative control group.The behavior and the expression levesl of let-7c-1,Bcl-2 protein and Caspase3 were evaluated at 28 days following PTZ.Results Compared to the negative group,the escape latency of epilepsy group was prolonged and the crossing times as well as the quadrant total distance in the target were reduced (P＜0.05).However,those parameters were not significantly different between the epilepsy group and the agmoir-control group (P＞0.05).Compared to the agmoir-control group,the escape latency of let-7c-1 agomir group was prolonged and the crossing times as well as the quadrant total distance in the target were reduced (P＜ 0.05).The expression levels of let-7c-1 and let-7c-1 were 1.35±0.32 in agmoir-control group and 62.53±21.01 in agomir group (F=50.97,P＜0.05).The expression levels of let-7c-1 were higher in let-7c-1 agomir group than in other groups (P＜0.05).Compared to the negative group,the expressions of Bcl-2 protein in other groups were decreased (P＜0.05) and the Caspase3 protein were increased (P＜0.05).Compared to the agomir-control group,the expression of Bcl-2 protein was significantly decreased and the expression of Caspase3 protein was significantly increased in let-7c-1 agomir group (P＜0.05).Conclusions The present study shows that let-7c-1 may impair the learning and memory of PTZ-induced epileptic rats through decreasing the Bcl-2 protein and increasing Caspase3 protein in the hippocampus.

Objective To evaluate hybrid therapy in patients with complex arteriosclerosis obliterans.Methods From Sep 2006 to Oct 2011,66 patients with complex arteriosclerosis obliterans were treated by hybrid therapy.A retrospective review was performed in all cases.Primary patency rate analyses were performed using Kaplan-Meier life tables.Results The technical success rate was 100％,and no patients died in perioperative period.12.1％ patients improved 3 levels,22.7％ patients improved 2 levels,and 65.2％ patients improved 1 level in Fontaine grade after procedures.The average ABI before and after procedures were 0.38 and 0.67 (P ＜ 0.05).2 patients died and other 64 patients completed 24 months follow-up.The primary patency rates and second patency rates were 75.0％ and 92.2％ at 24 months respectively.Primary patency rates of Fontaine Ⅱ patients were better than Fontaine Ⅲ and Ⅳ patients (P ＜ 0.05),and primary patency rates of Fontaine Ⅲ patients were better than Fontaine Ⅳ patients (P ＜ 0.05).Conclusions Hybrid therapy procedures provide an effective treatment in complex arteriosclerosis obliterans patients.

AIM: To investigate the protective effect of 6% Hydroxyethyl Starch(HES,200/(0.5)) on the brain of hemorrhagic-shocked rats.METHODS: Sprague-Dawley rats were randomly divided into sham operation group(group A),hemorrhagic shocked group(group B),whole blood return group(group C),HES at 2x shed blood volume(SBV) group(group D),HES at 1x SBV plus 1/2 SBV group(group E).Hemorrhagic Shock was induced by intermittently withdrawing blood from an iliac catheter.Except group A and B,the other groups were resuscitated with different liquids and lasted 30 min after 1h for shock.The extent of brain edema and inflammatory cells infiltrating was observed by Hematoxylin-Eosin staining,and the expression of nuclear factor kappa-B(NF-?B) in the rat brain and translocation of nucleus were detected using immunohistochemistry and Western blot method.RESULTS: Except the sham group,there were brain edema and inflammatory cells infiltrating in the other groups,which was more obvious in group C and D.The expression of NF-?B in the rat brain was significantly increased in group B and other groups as compared with those in group A(P

Objective:To explore potential differentiation from antigen-1 positive stem cells (Sca-1 +cells) in murine fetal liver tissue into cardiomyocytes in vivo. Methods: Sca-1 + cells from the murine fetal liver of 14.5 d were separated by Magnetic cell sorting (MACS).The sequence of the sex determination region of the Y chromosome was determined by PCR.Sca-1 + cells (2?10 3) of male mice were transfused to female mice receiving 8-12 weeks irradiation at a lethal dose of ?-ray(10 Gy) from a 60Co source.Two months later,the mice were killed and the hearts were removed to make slices. Fluorescence in situ hybridisation (FISH) tests with a Y chromosome probe were used to detect sex of the cells.Immunohistochemistry with antibodies of desmin,Flt-1,white blood cell common antigen CD45 and macrophage F 4/80 were used to detect tissue characteristics of cardiomyocytes. Results: Cells with Y chromosome were found in the cardiomyocytes of female mice transplanted with Sca-1 + cells,and showed phenotypic characteristics of Desmin +/Flt-1-/CD45-/F- 4/80 at the same time.Conclusion: Sca-1 + cells from the murine fetal liver are mostly hematopoietic stem cells,having the potential of differentiating into cardiomyocytes.