Objective:To design a system of computing and storing wound area. Methods:Visual C++6.0 platform has been used to develop the application.With image processing and by means of ADO, the wound area can be calculated and stored in the Access 2003 databse. Results:The system has achieved the expected effect which can be proved by measuring animal model and saving reaults. It can calculate the wound area and save the relative information fast. Conclusion:This system is qualified for computing and storing wound area and can search the information stored as well. Not only can it provide doctors with accurate information,but also bring patients convenience.

[Objective] To observe the effects of Oysters, Abalone shel , Concha arcae, Mother-of-pearl, Concha cypraeae, Clamshel , these six testacean TCM on the goiter rats. [Method] 72 healthy SD rats were randomly divided into nine groups:normal group, model group, positive group and six testacean TCM groups. Rats were given daily propylthiouracil (0.15 g·kg-1), continuous modeling for 10d, weighing thyroid wet weight,assaying T3, T4 and TSH contents in serum by ELISA, observing the morphological changes of thyroid under light microscope. [Result] Rats in the model group compared with normal group in-creased the relative weight of thyroid, reduced the content of T3,T4, and there was a significant difference( P<0.01), thyroid fol icular epithelial cellhyperplasia was obvious;compared with the model group, Oysters and Clamshel group made the content of T3,T4 in rat rebound, and there was a significant difference (P<0.05);Oyster and Clamshel group’s thyroid fol icular cellhyperplasia were not obvious, while the hyperplasia had different levels for the other 4 kinds of testacean TCM. [Conclusion] Oysters and Clamshel were better to improve the thyroid function of the goiter model rats.

Objective To analyze the relationship between atrial fibrillation (AF) and plasma mononuclear cells or cardiac func-tion .Methods From April 2010 to Jun 2013 ,298 patients with AF from department of cardiology in our hospital were selected as study group ,300 patients without AF were selected as control group in the same period .Both group and the subgroup were analy-sised by t test .Results Plasma monocyte NT-proBNP ,left atrial diameter in AF group were significantly higher than the control group(P<0 .05) ,LVEF was lower than the control group ,the statistical differences between the two groups (P<0 .05) .Compare HF combine AF with HF only ,the NT-proBNP and left atrial diameter were significantly higher in former and the LVEF is lower than the latter ,both with the difference statistical significantly (P< 0 .05) .Merge heart failure combined with atrial fibrillation (HF+AF) and heart failure combined sinus rhythm (HF+ SR) group ,the former in the NT-proBNP and left atrial diameterwere significantly higher than the latter ,LVEF lower than the latter ,the difference was statistically difference (P<0 .05) .Conclusion In the development of AF ,the monocytes may be involved in the occurrence and development of AF .

?Objective:To study apoptosis and apoptotic mechanisms of buccal carcinoma cells(BCCs) induced by anticancer agents.Methods:34 cases of buccal carcinoma were treated with pinyangmycin or pinyangmycin combined with other anticancer agents.The samples of the lesions were examined by in situ terminal deoxynucleotidyl transferase (TDT) mediated dUTP nick end labeling (TUNEL) before and after chemotherapy.Apoptotic cells were detected in parraffin embedded buccal cancer specimens from the patients.Apoptotic index (AI) were respectively quantified in prechemotherapy and postchemotherapy specimens of each patient.Fas expression was examined by SP immunohistochemical staining in each specimen.Results:The mean of AI in the samples of prechemotherapy and in those of postchemotherapy were 0.38 and 0.91 ( P

objective: To evaluate the effects of the pendulum appliance on arch length, arch width, overbite and overjet. Methods: Pendulum appliances were applied for distalization of maxillary molars in 37 patients whith class Ⅱ malocclusion and 1-Ⅱ degree crowded dentition in upper dental arch. Pretreatment and posttreatment model analysis was conducted. Results: 37 patients treated for 18.2?4.5 weeks with pendulum appliance were accomplished . The increase of arch arc length between the maxillary first molars was (17.36?4.38) mm, the decrease of anterior arch width (-3.04?1.59) mm, and the decrease of posterior arch width (-1.29?2.01) mm . Differences between the pretreatment and posttreatment were significant, including arch arc length between the maxillary first molars ( P

Objective To analyze the reasons of the common complications happened after the operation of congenital syndactyly and to find the method of prophylaxis and treatment,and to improve the effects of treatment.Methods To classify the reasons of the complications happened after the operation of congenital syndactyly in 34 cases.Results The reasons of the complications included that the patients were not operated by special course of study doctors in 9; operation at an inappropriate opportunity in 8;the method of web taken shape was improper in 7;the method of nail taken shape was improper in 4; the fixation and training was unsuitable in 6. Conclusions The complications happened after the operation of congenital syndactyly will be reduced much , provided that grasping the right opportunity and selecting the proper method of web or finger tip taken shape before operation , taking care of the transformations of blood vessel and nerve in operation , fixing firmly and training appropriately after operation.

[Objective]To investigate the osteogenic ability in vivo of mesenchymal stem cells(MSCs).[Method]Ten-fifteen ml bone marrow aspirates were harvested from the iliac crest of sheep and enriched for MSCs by density gradient centrifugation over a Percoll cushion(1.073 g/ml).After cultured and proliferated,the tissue-engineered bones were constructed with these cells and seeded onto porous 13-tricalcium phosphate ceramics(13-TCP).Then,the constructs were implanted into left metatarsus defect(21 mm in length)of 8 sheep as an experimental group.Porous ?-TCP composed with bone marrow was implanted into defects of same size and position in 8 sheep as a control group.Sheep were sacrificed in the 6th,12th,and 24th week postoperatively,and the implanted samples were examined by radiograph,histology,and biomechanical analysis.[Result]New bone tissue was observed either radiographically or histologically at the defect area of experimental group as early as the 6th week postoperatively;but was not observed in the control group.Because of the new bone formed in a direct manner without progression through a cartilaginous process intermediately,osteoid tissue,woven bone and lamellar bone,occurred earlier in the experimental group than in the control group,in which the bone defects were repaired in a "creep substitution" manner.At the 24th week,radiographs and biomechanical tests revealed an almost complete repair of the defect in experimental group,but only a partial repair in the control group.[Conclusion]MSCs have good reproductive activity not only in vitro,but also in vivo.The new bone formed in a direct manner without progression through a cartilaginous process intermediately when MSCs were transplanted in vivo.The results demonstrated that MSCs was an excellent seed cells for bone tissue engineering.

Bone Density ; physiology ; Bone Development ; physiology ; Bone and Bones ; diagnostic imaging ; Child ; Humans ; Nutrition Assessment ; Ultrasonography

Objective To discuss the levels of inhibitory protein Ga-2 (Gαi2) in hippocampus of brain and the effects of Gαi2 on neuronal intracellular Ca2+ level in cerebral ischemia reperfusion injury model of rats.Methods Ninety SD rats were randomly (random number) assigned to sham group (n =30),ischemic-reperfusion (IR) group (n =30),Pertussis toxin (PT) group (n =30).The blood flow of right common carotid artery of rat was blocked for 90 min to make ischemia reperfusion model.The levels of Gαi2 in hippocampus was assayed by immunohistochemistry and Western blotting after ischemia reperfusion for 6,12,24 h in each group.The average fluorescence values of intracellular Ca2+ levels in hippocampus of rats in three groups were detected by using Flow CytoMeter (FCM).Neuronal cell apoptosis was measured by TUNEL.Results After restoration of middle cerebral artery blood flow for different lengths of time,the levels of hippocampus Gαi2 and Ca2+ levels in IR group were significantly higher than those in Sham group (P ＜0.01).The hippocampus Ca2+ levels in PT group were higher than those in IR group (P ＜ 0.01).The apoptotic rates of neurons in PT group were lower than those in IR group (P ＜0.05).Conclusions The level of Gαi2 in cerebral ischemia reperfusion injury model was increased.Gαi2 might reduce the calcium ion concentration of neurons after cerebral ischemia and rcduce the neuronal cell apoptosis in this model.Gαi2 might play a role in protecting neuron from cerebral ischemia reperfusion injury.

Objective:To evaluate effects of traction arch combined with protraction on dental and skeletal changes in patients with Angel Ⅲ malocclusion in mixed dentition.Methods:Seventeen patients with Angle Ⅲ malocclusion were selected and treated with traction arch combined with protraction. Cephalometric radiographs were taken 6 months before the initiation of treatment, at the initiation of treatment and 6 months after treatment.Cephalometric analysis was used to examine the dental and skeletal changes.Results:Significant changes of maxillary anterior displacement and mandibular downward and backward rotation were observed in the seventeen patients:SNA increased 2.6?,A point moved forward 3.1 mm. The overjet correction of anterior teeth was 5.2 mm, 63% of the overjet correction was due to skeletal changes and 37% due to dental changes. Conclusion:Traction arch combined with protraction is a simple and effective apparatus in the treatment of Angle Ⅲ malocclusion.