Objective To investigate the role of sex chaperone on self-acceptance,anxiety,depression and coping strategy in stable phase extensive burn patients'.Methods According to the different role of accompanies,the extensive burn patients in stable phase were divided into sex chaperone accompany groups(treatment group)and non-sex chaperone accompany group(control group).They were assessed by self-acceptance questionnaire(SAQ),self-rating anxiety scale(SAS),self-rating depression scale(SDS)and medical coping modes questionnaire(MCMQ).The results were statistically analyzed.Results The treatment group patients'results are as follows:SAQ total score was(41.37±6.98),self-acceptance factor score was(20.56±4.87),self-evaluation factor score was(22.35±5.82),MCMQ facing factor score was(21.80±4.07),MCMQ avoiding factor score was(22.35±5.82).The results of treatment group patients were significantly higher than those in control group,it had significant differences(P<0.05 or P<0.01);while the treatment group patients'scores of SAS,SDS,MCMQ yield factor were significantly lower that those in the contorl group.The self-acceptance factor score appeared to be negative correlation with the SAS score,SDS score and MCMQ yield factor score,while to be positive correlation with MCMQ facing/avoiding factor score.Conclusion Sex chaperone'accompany can eliminate the extensive burn patients'tension and the effect of adverse psychological factors,and meet family love needs of patients,then improve the level of patient self-acceptance,reverse the negative emotion of patients,and guide patients in a positive way to face the disease.

The pharmacokinetics of ketamine were studied in 8 preschool children,aged 3.5～6 years. The ketamine was administrated by I. V. infusion at a dose of 3mg?kg~(-1) in one hour constantly. The plasma concentrations of ketamine were measured by high performance liquid chromatography. The disposition of intravenous infusion with ketamine could be described by two-compartment open model, and the variables of pharmacokinetics of ketamine were as follows: T_(1/2?)=0.15?0.04 hours,T_(1/2?)=2.84?0.44 hours,Cl=0.34?0.06L?kg~(-1)?h~(-1), AUC=9.21?1.00mg/L?h, and V_d=0.66?0.29 L?kg~(-1). This suggests that intravenous infusion of ketamine at 3mg?kg~(-1)?h~(-1) is safe for the maintenance of general anesthesia in preschool children, attention must he paid to the children anesthetized with long period of ketamine infusion because recovery may be delayed due to the prolonged elimination half-life.

Objective: To determine the influence of ?-hydroxybutyric sodium (7-OH)on the pharmacokinetics of ketamine infusion in preschool children. Method: Sixteen patients were randomly assigned to group Ⅱ (Ketamine) and group Ⅱ (Ketamine plus ?-OH). After trachea intubation,0.05% ketamine was infused in both groups at 3mg?kg~(-1)?h~(-1) and ?-OH 125mg?kg~(-1) only in group Ⅱ. The plasma concentration of ketamine was measured using high performance liquid chromatography. Pharmacokinetic parameters were calculated using 3P_(87) software composed by Chinese Pharmacologic Society. Result: In both groups,pharmaeokinetics could be descrided as two-compartment open model. The T_(1/2)? and the Cl differ between group Ⅰ and group Ⅱ (P

Objective Objective To study the effect of Mycobacterium tuberculosis (Mtb) on IFN-? signaling pathway in macrophages. Methods Humans and mice macrophages were isolated and infected by Mtb, condition medium were collected after a few days culture; Macrophage surface expression of Fc?RI (human) or MHC II (mouse) was measured by flow cytometry to explore the effect of condition medium on macrophage response to IFN-?. Results Surface expression of Fc?RI in human macrophages cultured in condition medium was significantly decreased compared to those without condition medium (p

A highly sensitive and selective method for specific DNA sequence detection is developed using a non-labeled molecular beacon (MB) and a nucleic acid dye Hoechst 33258. It is demonstrated by a specific DNA sequence of wild-type HBV as a model system. In this strategy, the stem of MB is completely designed as C/G base pairs. In the absence of target DNA, the interaction between Hoechst 33258 and the MBs is very weak,and the fluorescence signals of Hoechst 33258 is very low. In the presence of target DNA, the MBs hybridize with the target DNA and form double-stranded structure. Hoechst 33258 binds to dsDNA, and the fluorescence intensity is significantly enhanced. Under the optimum conditions, the fluorescence intensity of Hoechst 33258 exhibits good linear dependence on target DNA concentration in the range of 2 × 10-10-2 × 10-8 mol/L. The fitted regression equation is △I=3. 3439C(10-10 mol/L) ﹢18. 6949(R2=0. 9982) with a correlation coefficient of 0. 9982 (R2), and the detection limit is 9 × 10-11 mol/L (3σ). The proposed method has good precision, simple operation, fast detection speed, low detection limit, high accuracy and high sensitivity.

AIM: To establish HPLC fingerprint of Viticis Fructus. METHODS: Hypersi ODS2 column was used, with mixtures of methanol and water as mobile phase in a gradient mode. The detection wavelength was set at 270 nm . The flow rate was 1.0 mL ?min -1 . RESULTS: The RSD% of precision and reproducibility was less than 5%. CONCLUSION: The method can be used for quality control of Viticis Fructus

Objective To explore the expression of cyclooxygenase-2 (COX-2) in different epidermal tumors and its significance. Method The expression of COX-2 was detected by immunohistochemical staining of skin specimens from 10 cases of basal cell carcinoma (BCC), 8 squamous cell carcinoma (SCC), 8 Bowen′s disease (BD), 12 seborrheic keratosis (SK) and 10 normal volunteers. Results Compared with normal epidermal cells, COX-2 expression was significantly increased in BCC, SCC and BD cells. Especially, COX-2 expression in SCC was the most intensive. COX-2 expression in SK cells was similar to that of normal epidermal cells. Conclusion The up-regulation of COX-2 expression may play a potential role in the pathogenesis of epidermal tumors.

0.05). The incidence of restenosis and major adverse cardiovascular events were significantly less in the hyperbaric oxygen group than that in the control group (6.67% vs 22.58% for restenosis, and 8.82% vs 38.24% for major adverse cardiovascular events; P0.05). No severe adverse effect was found during hyperbaric oxygen therapy in the hyperbaric oxygen group. Conclusion Hyperbaric oxygen therapy is effective and safe in preventing restenosis after intracoronary stenting.

Objective To establish the fingerprint analysis method of Schisandrae Chinensis Fructus by HPLC; To analyze the similarity on the fingerprints of Schisandrae Chinensis Fructus from different producing areas. Methods The chromatographic separation was performed by HPLC on a Diamonsil C18 column (250 mm×4.6 mm, 5 μm). Acetonitrile-water was used as gradient mobile phase. The flow rate was 1.0 mL/min. The column temperature was maintained at 30℃. The detection wavelength was set at 254 nm.Results The HPLC fingerprint analysis method of Schisandrae Chinensis Fructus was established. Twenty-nine common fingerprint peaks were identified. The similarities of the fingerprints of ten samples from different producing areas were above 0.95.Conclusion The method is simple and reliable, which can provide a scientific basis for quality evaluation of Schisandrae Chinensis Fructus.

A plasmids of continuous expressing shRNAs targeting the NDV NA-1 Phosphoprotein (P) gene was designed.Virus titration,Real Time RT-PCR,CPE indicated that P-specific siRNA could inhibit virus replication at 36 h post-virus infection.In future studies,a combination of siRNAs targeting the NP and L gene may be used as a tool to study NDV replication and antiviral therapy.