1.Inhibition of Newcastle disease virus (NDV) replication by RNA interference targeting phosphoprotein (P) gene in CEF
Lianzhi MU ; Zhuang DING ; Yanlong CONG ; Renfu YIN ; Mei LIU ; Changqing WANG ; Shaoli LI ; Mimi QIU
Chinese Journal of Veterinary Science 2009;29(7):841-844
A plasmids of continuous expressing shRNAs targeting the NDV NA-1 Phosphoprotein (P) gene was designed.Virus titration,Real Time RT-PCR,CPE indicated that P-specific siRNA could inhibit virus replication at 36 h post-virus infection.In future studies,a combination of siRNAs targeting the NP and L gene may be used as a tool to study NDV replication and antiviral therapy.
2.Isolation,identification and sequence analysis of the strain JL/07/SW with deletion mutations of PRRSV
Zhijie LI ; Zhuang DING ; Keyin MENG ; Hua XUAN ; Guiping WANG ; Enpeng GAO ; Yanlong CONG ; Lianzhi MU
Chinese Journal of Veterinary Science 2009;29(7):830-835
One strain of PRRSV was isolated from tissue of piglets died of obvious respiratory syndrome and high fever.It was identified as a strain of American type PRRSV by serological test and gene sequencing.According to American type PRRSV VR-2332,we designed 3 pairs of primers to aim directly at N gene,GP5 gene and variation sequence of NSP2.The results of sequence analysis indicated a discontinuous deletion of 30 amino acids in nonstructural protein 2 (NSP2).N and GP5 gene is conservative relatively.
Result Analysis
Print
Save
E-mail