Objective To investigate the regulation of Sertoli cells on differentiation balance of allogeneic regulatory T cell (Treg)and T helper cell(Th17).Methods Sertoli cells ,bone marrow‐derived dendritic cells(DC) and T lymphocytes were iso‐latedandculturedinvitro.SertolicellsandDCcellswereco‐culturedwithallogeneicTcells,respectively.MorphologyofSertoli cells were verified under the light microscope and electron microscope. The expression of major histocompatibility complex (MHC)‐Ⅱ and B7‐H1 in Sertoli cells was detected by Western blot.Concentration of cytokines[transforming growth factor (TGF)‐β1 and IL‐17A etc. ] in supernatant was determined by enzyme linked immunosorbent assay(ELISA). The frequency of Treg cells differentiated from na?ve T cells was estimated by flow cytometry.Results Sertoli cells were successfully isolated and cultured in vitro.Western blot results showed the expression of MHC‐Ⅱ and B7‐H1 on Sertoli cells surface was increased significantly ,when Sertoli cells were co‐cultured with allogeneic na?ve CD4+ T cells(CD4+ CD62 L+CD25)in vitro.ELISA re‐sults showed the TGF‐β1 concentration in culture supernatant was significantly increased [from (174.89 ± 23.51) pg/mL to (823.10 ± 59.07) pg/mL ,P<0.05] .Flow cytometry results showed Na?ve T cells(CD4+CD62 L+CD25-)were induced to dif‐ferentiate into CD25+ Foxp3+ T cells(from <1.0% up to 27.6% )by co‐cultured with Sertoli cells.And incubation with Sertoli/Treg supernatant(STS)significantly reduced the concentration of IL‐17A in the mixed culture supernatant of DC cells /Na?ve CD4+ T cell[from (361.58 ± 80.27) pg/mL to (137.54 ± 52.19) pg/mL ,P<0.05]. The percentage of IL‐17A+ T cells subset was also significantly decreased(27.2% to 17.9% ,P<0.05).Conclusion The anti‐inflammatory cytokines and immune sup‐
pressive cytokines derived from Sertoli cells can induce allogeneic na?ve T cells to differentiate into CD25+ Foxp3+ Treg cells in vitro.Sertoli cells can regulate the differentiation balance of allogeneic Treg/Th17 to induce the immune tolerance.

Objective To observe the clinical effects of medicine-separated moxibustion in treating renal edema.Methods Totally 134 patients with renal edema in the first affiliated hospital of Hunan University of Chinese Medicine from July 2014 to June 2015 were selected and divided into the observation group and the control group by random number table.Patients in the observation group received conventional nursing and medicine-separated moxibustion treatment,while patients in the control group received conventional treatment and nursing.The clinical effect and patient satisfaction were compared in two groups.Results The total efficiency of hematuria was 96.97％ and negative conversion rate was 54.55％ in the observation group.The total efficiency of hematuria was 80.56％ and negative conversion rate was 36.11％ in the control group.The data in the observation group were significantly better than those of the control group (x2 =4.526,P＜0.05).The total efficiency of proteinuria was 92.68％ and negative conversion rate was 58.54％ in the observation group.The total efficiency of hematuria was 79.07％ and negative conversion rate was 46.51％ in the control group.Data in the observation group were significantly better than those of the control group (x2=5.591,P＜0.05).The weights of patients were lost,urine volume in 24 hours increased and muscle circumference decreased.Besides that,the effect in the observation group was better than that of the control group (P＜0.05).The patient satisfaction of the observation group (100.00％) was significantly better than that in the control group (78.79％) (P＜0.01).Conclusions The medicine-separated moxibustion combined with western medicine can help to improve the clinical efficacy and negative conversion rates of hematuria and proteinuria;reduce the patient's weight,increase urine volume in 24 hours;and significantly reduce leg circumference in treating the patients with renal edema.in addition,it can greatly reduce the relapse of edema,and it is beneficial to improve patient satisfaction,which should be applied to the clinical treatment.