1.Optimization and effectiveness of bladder irrigation management process in patients after greenlight photoselective vaporization of the prostate
Lei SHI ; Lianxia GEN ; Hui XU ; Jiaojiao LIU ; Junxiao CHEN ; Hongjun WANG ; Jianhong LI
Chinese Journal of Modern Nursing 2020;26(16):2193-2198
Objective:To explore and optimize the management process of bladder irrigation for patients after greenlight photoselective vaporization (PVP) , reduce the occurrence of postoperative complications, and improve the satisfaction of patients and nurses for bladder irrigation.Methods:Totally 315 patients undergoing PVP in the Department of Urinary Surgery, the Second Hospital of Hebei Medical University from May 2017 to April 2019 were selected using purposive sampling. A total of 153 patients admitted from May 2017 to April 2018 were divided into the control group, who received routine bladder irrigation management, while 162 patients admitted from May 2018 to April 2019 were divided into the observation group, who received bladder irrigation management by the failure mode and effect analysis. The incidence of complications such as bladder spasm and urinary tract obstruction, the duration of continuous bladder irrigation, and patient satisfaction were compared between the two groups, and the nurse satisfaction before and after the process optimization was compared.Results:After the process optimization, the incidence of bladder spasm decreased from 17.0% (26/153) to 8.0% (13/162) , the incidence of urinary tract obstruction decreased from 11.8% (18/153) to 3.7% (6/162) , and the differences were statistically significant (χ 2=5.835, 7.264; P<0.05) . The duration of bladder irrigation was shortened from (25.311±2.295) h to (22.422±1.481) h, and the difference was statistically significant ( t=13.348, P<0.05) . After optimizing the bladder irrigation management process, the nurse satisfaction score was (34.221±5.124) , and the patient satisfaction score was (37.113±6.108) , both higher than those before the optimization, and the differences were statistically significant ( t=19.817, 14.546; P<0.05) . Conclusions:Optimizing the bladder irrigation management process for patients after PVP using the failure mode and effect analysis can reduce the incidence of postoperative bladder irrigation complications, shorten the duration of bladder irrigation, and improve patient and nurse satisfaction.
2.Prevalence of Mycoplasma pneumoniae infection in children in Hengyang and comparison of three methods for genotyping
Lianxia LIU ; 421001 衡阳,南华大学附属第一医院输血科 ; Jinhong XIAO ; Wei TIAN ; Lan YU ; Xiaoxing YOU ; Yanhua ZENG ; Cuiming ZHU
Chinese Journal of Microbiology and Immunology 2017;37(11):862-868
Objective To investigate the prevalence of Mycoplasma pneumoniae ( Mp) infection in children in Hengyang from 2013 to 2016 and to analyze the p1 genotypes of the isolated Mp strains by using polymerase chain reaction-restriction fragment length polymorphism ( PCR-RFLP) , nested polymerase chain reaction (nPCR) and rapid-cycle polymerase chain reaction (Rapid-Cycle PCR).Methods Throat swab samples of children with acute respiratory tract infection were collected from four hospitals in Hengyang , Hu-nan Province from 2013 to 2016.Mp strains in these samples were identified by PCR amplification of the 16S rRNA gene.PCR-RFLP, nPCR and Rapid-Cycle PCR were performed for Mp p1 genotyping in order to fur-ther analyze the genotypes of Mp strains circulating in Hengyang .Results A total of 109 clinical strains of Mp were identified from the 984 throat swab samples .The sensitivities of PCR-RFLP and nPCR for genoty-ping MP strains were both 100%, while that of rapid-Cycle PCR was 98 .17%.All of the three methods showed 100%specificity for genotyping.Of all isolated Mp strains, 78.90% were p1 gene type Ⅰ and 21.10%were p1 gene typeⅡ(t=93.239, P=0.01).From 2013 to 2016, the annual isolation rates of p1 gene type Ⅰ and type Ⅱ strains were 93.10%, 87.5%, 76.92%, 65.79% and 6.90%, 12.5%, 23.08%, 34.21%, respectively.The rate of Mp p1 gene type Ⅰinfection decreased over year , while that of p1 gene type Ⅱinfection increased gradually .Conclusion PCR-RFLP, nPCR and rapid-Cycle PCR are reliable for genotyping of Mp p1 gene.The predominant genotype of Mp strains circulating in Hengyang is p 1 gene type Ⅰ, but the incidence of p 1 gene type Ⅱinfection gradually increases from 2013 to 2016 .