Objective To Investigate the effects of antipsoriatic drugs on 5 lipoxygenase(5－LO) and set up a relevant pharmacodynamic method. Methods 5－ LO products, leukotriene B4 (LTB4) and 5 hydroxyeicosatetraenoic(5－HETE), we re determined by RP－HPLC to represent 5－LO activity. Results Cyclosporin A( CyA) and triptolide(T0) inhibited the production of LTB4 and 5－HETE in a dose dependent manner, while erythromycin did without dose dependence. The 50％ inhibitory concentration values(IC50) of CyA inhibiting LTB4 and 5－HETE were 3 8.0? g/mL and 0.96? g/mL, respectively. The IC50 of T0 inhibiting LTB4 and 5－ HETE were 2.3? 10－6? g/mL and 1.14? 10－6? g/mL, respectively. Conclusio ns The anti inflammatory effect of Tripterygium wilfordii Hook.f. may be partl y explained by its inhibition of 5－LO activity. The anti inflammatory effect of CyA has no clinical significance since the inhibitory concentration of CyA h as exceeded its pharmacological limitation. Erythromycin has no effect on 5－LO activity.

Objective To investigate the effects of some compounds,bestatin,tripdiolide,etc,on leukotriene A4hydrolase activity.Methods LTB4,product of leukotriene A4hydrolase,was determined by reverse phase high performance liquid chromatography(PR-HPLC).Bestatin,an inhibitor of LTA4hydrolase was used as a positive control.Results Tripdiolide could inhibit LTA4hydrolase activity in a dose-depen-dent pattern.The50%inhibitory concentration values(IC 50 )was2.58?10 -5 ?g/mL.The other compounds,an-thralin,cyclosporin A,tretinoin,calcipotriene,clobetasol propionate,methotrexate,and erythromycin,had no effects.Conclusions Tripdiolide possibly has anti-inflammatory effect through down-regulation of leukotriene A4hydrolase in psoriasis.

Polydimethylsiloxane (PDMS) and hydroxyapatite (HA) were combined in our laboratory to fabricate an elastic porous cell scaffold with pore-forming agent, and then the scaffold was used as culture media for rat bone marrow derived mesenchymal stem cells (rBMSCs). Different porous materials (square and circular in shape) were prepared by different pore-forming agents (NaCl or paraffin spheres) with adjustable porosity (62%-76%). The HA crystals grew on the wall of hole when the material was exposed to SBF solutions, showing its biocompatibility and ability to support the cells to attach on the materials.
Animals ; Biocompatible Materials ; chemistry ; Dimethylpolysiloxanes ; chemistry ; Durapatite ; chemistry ; Mesenchymal Stromal Cells ; cytology ; Porosity ; Rats ; Tissue Scaffolds

Objective To apply 3C3R model in problem design of PBL for pediatrics teaching.Methods The 3C3R model comprises two classes of components:core components and processing components.Core components of the model are 3C,which are content,context and connection while processing components are 3R,which are researching,reasoning and reflecting.3C3R model was used in the problem design for the PBL case of ‘ Why the mouth of Baobao became purple when he was crying?' Totally 76 eight-year program medical students and 7 tutors were enrolled as teaching object.The anonymous questionnaires from the students were collected for assessment of PBL teaching.Results The percentage of students with scores ≥4 for content in PLB problem design was 90.8％,for context was 80.3％,for connection was 64.5％,for researching was 81.6％,for reasoning was 69.7％ and for reflecting was 40.8％.The percentage of tutors with scores ≥4 for content in PLB problem design was 100％,for context was 71.4％,for connection was 28.6％,for researching was 71.4％,for reasoning was100％,for reflecting was57.1％.Both students and tutors held a positive attitude towards the component of content,context,researching and reasoning in problem design model.But the components of connection and reflection needed to be improved.Conclusion The 3C3R model is helpful for problem design of PBL in pediatric teaching.

Cell mechanics is a new interdiscipline, which focuses on the relationship between cell mechanical properties and cell behaviors, as well as physiological processes in tissues, organs and the body. Through the perception of and response to mechanical signals, cells participate in physiological activities of skin, bones, eyes and other organs, and the occurrence and development of diseases, including the regeneration, reconstruction and adaptive changes of skin tissues and the pathogenesis of various skin diseases. Cell mechanics has become a hot spot in the research of skin diseases in recent years. This review summarizes recent progress in cell mechanics-associated basic research, as well as in its research and application in scars, bedsores, vitiligo, skin tumors, hair diseases, etc., aiming to provide possible methods and strategies for the treatment of skin diseases.

Background/Aims: The aim of this study was to analyze the chronological changes in postoperative complications in surgical ulcerative colitis patients over the past decade in China and to investigate the potential parameters that contributed to the changes. Methods: Ulcerative colitis patients who underwent surgery during 2008–2017 were retrospectively enrolled from 13 hospitals in China. Postoperative complications were compared among different operation years. Risk factors for complications were identified by logistic regression analysis. Results: A total of 446 surgical ulcerative colitis patients were analyzed. Fewer short-term complications (24.8% vs. 41.0%, P=0.001) and more laparoscopic surgeries (66.4% vs. 25.0%, P<0.001) were found among patients who received surgery during 2014–2017 than 2008–2013. Logistic regression suggested that independent protective factors against short-term complications were a higher preoperative body mass index (odds ratio [OR], 0.870; 95% confidence interval [CI], 0.785–0.964; P=0.008), laparoscopic surgery (OR, 0.391; 95% CI, 0.217–0.705; P=0.002) and elective surgery (OR, 0.213; 95% CI, 0.067–0.675; P=0.009). The chronological decrease in short-term complications was associated with an increase in laparoscopic surgery. Conclusions Our data revealed a downward trend of short-term postoperative complications among surgical ulcerative colitis patients in China during the past decade, which may be due to the promotion of minimally invasive techniques among Chinese surgeons.

Advanced glycation end-products (AGEs) are the products of non-enzymatic reactions between free amino groups of macromolecules and reducing sugars. AGEs accumulation in bone tissues changes the activity and function of bone cells by binding to their surface receptors, causing abnormalities in the process of bone remodeling. AGEs accumulation can also change the original structure and mineral deposition of bone collagen, affect the micro-mechanical properties of bone tissues, and further reduce bone strength and toughness, increase the bone fracture risk, which will lead to bone diseases and do great harm to human health. This article summarized the causes of AGEs and their detection methods, and reviewed previous studies about the effects of AGEs accumulation on bone biomechanics at micro and macro levels, so as to provide references for the early diagnosis and treatment of bone diseases in clinic.

On the earth,the majority of cellular functions are regulated by mechanical loads.The effects of cell response to mechanical loads under microgravity on the adaptive changes of physiological functions in space cannot be ignored.The cytoskeleton is widely considered as one of the key structures through which cells sense gravity variation and respond to mechanical loads.In this review,the research progress on the changes and mechanisms of the'cytoskeleton-LINC complex-nuclear lamina'pathway in cellular mechanoperception and mechanotransmission under microgravity is summarized,and the future research is prospected.

Objective To study the effect of simulated microgravity on activity of the store-operated calcium (SOC) channels in osteocytes and its possible mechanism, so as to elucidate the potential mechanism of weightlessness bone loss. Methods Osteocytes (MLO-Y4) as the experimental subjects were divided into simulated microgravity (SM) group and normal gravity group (CON). After rotating for 24 h and 48 h, confocal microscope was used to detect the intracellular calcium ion concentration level to reflect activity of the SOC channels after thapsigargin (TG)-induced endoplasmic reticulum (ER) depletion. Immunofluorescence staining was used to observe the distribution of ER membrane protein IP3R and spectrin membrane skeleton, in order to preliminarily explore the possible mechanism of functional changes of SOC channels. Results During the period of calcium release from ER, ［Ca2+］i had no significant difference between SM group and CON group for 24 h and 48 h; while during the period of extracellular calcium influx by SOC channels, ［Ca2+］i of SM group had significant differences in the first 4 minutes for 24 h, as well as in the whole time for 48 h. Compared with CON group, the spectrin membrane skeleton of SM group was gathered at the rim of membrane, while ER membrane protein IP3R of SM group was gathered at the nuclear envelope of ER. These two tendencies were more obvious for 48 h. Conclusions The stimulated microgravity could inhibit activity of SOC channels in osteocytes. Changes in the distribution of the spectrin membrane skeleton and ER membrane protein IP3R under the simulated microgravity might reduce the activity of SOC channels by affecting the conformation coupling process between the membrane and ER.