OBJECTIVEWe aim to evaluate the cytotoxicity and antibacterial effects of Silagum-Comfort (SLC) silicone-based soft liner varnish containing Ag/TiO2 in vitro.

METHODSSilicone-based liner circular specimens, with a diameter of 10 mm and a thickness of 2 mm, were created and divided into six groups randomly. Ag/TiO2 was incorporated into the SLC varnish based on the agent/material weight rate of 0, 0.5%, 1.0%, 1.5%, 2.0%, and 2.5%. The mixed varnish was used to polish the SLC specimens. Antibacterial specimens (ATSLC) were created. Methyl thiazolyl diphenyl-tetrazolium bromide assay method was used to evaluate the 3T3 cell cytotoxicity of ATSLC in vitro. Surface roughness (Ra) measurements of ATSLC were obtained. The antibacterial effect of ATSLC on Candida albicans was examined using the pelliclesticking method.

RESULTSThe cytotoxicity of ATSLC was graded from 0 to 2. The Ra of ATSLC increased when Ag/TiO2 agent concentration increased. However, the differences were not statistically significant compared with the 0 group. With increasing Ag/TiO2 concentration, the antibacterial agent concentration and the antibacterial rate of ATSLC also increased. When the Ag/TiO2 antibacterial agent concentration reached 2.0% and 2.5%, the antibacterial rate increased to 97.5% and 96.5, respectively.

CONCLUSIONVarnish containing Ag/TiO2 has excellent antibacterial effect and can significantly improve the antibacterial effect of silicone-based soft liner.

Anti-Bacterial Agents ; Candida albicans ; In Vitro Techniques ; Paint ; Silicones ; Titanium

OBJECTIVETo compare shear bond strength (SBS) between two types of silicone soft liner and polymethyl methacrylate (PMMA) under the condition of heat curing and room temperature curing.

METHODSA total of 48 PMMA specimens (50 mm x 10 mm x 3 mm) were made by water-bath heating method, and randomly divided into four groups. By using Ufi Gel P (UGP) as soft liner material, group A1 was prepared under heat curing, and group A2 was prepared under room temperature curing. To form the other two groups, Silagum-Comfort (SLC) as soft-liner material was used. Group B1 was prepared under heat curing, and group B2 was prepared under room temperature curing. Shear bond strength (SBS) was tested by using the electronic universal testing machine. The adhesives layer and surface of silastic and PMMA were observed by optical microscope and scanning electron microscopy (SEM).

RESULTSThe SBS of groups A1, A2, B1, B2 were (2.39 +/- 0.24), (1.74 +/- 0.27), (3.09 +/- 0.26), and (2.21 +/- 0.29) MPa, respectively. Significant differences were found between A1 and A2, B1 and B2, A1 and B1, and A2 and B2 (P < 0.05). Optical microscope showed numerous bubbles in the cured UGP, and no air bubbles in the SLC. The surface of PMMA was rough. SEM images showed that each group had continual consistent adhesive interface and a whisker hump on the adhesive layer of A2 and B2.

CONCLUSIONThe SBS ofUGP, SLC, and PMMA achieved minimum clinical standard of 0.44 MPa. The SBS of UGP and PMMA were higher than that of SLC and PMMA. The polymerization method of heat curing was higher than room temperature curing.

Dental Bonding ; Denture Liners ; Dimethylpolysiloxanes ; Materials Testing ; Polymerization ; Polymethyl Methacrylate ; Silicone Elastomers ; Silicones ; Tensile Strength

Objective:To evaluate the cytotoxicity of colorized zirconia ceramics.Methods:Zirconia ceramic 3Y-TZP blocks were colorized by NH4 VO3 ,FeCl3 ,Co(NO3 )3 ,Ni(NO3 )3 and MoCl3 respectively.The cytotoxicity of colorized zirconia to L-929 cells was examined by immersion method and MTT assay.Results:During 3 days culture,L929 cells in all groups showed similar prolifera-tion and normal morphology.The toxicity gradation of all groups was 0 -I.Conclusion:Zirconia ceramics colorized with the 5 kinds of colorants has no cytotoxicity.

94 cases with 148 abutments treated by all-ceramic restoration in premaxillary teeth region were randomly divided into 4 groups, and the impressions were prepared by one-step method,double curettage method,double polish method and double microfilm method respec-tively using 3M ESPE Vinyl polysiloxane as impression material.The cervical margin precision of the impressions was compared.Four kinds of impression method can produce qualified impression.Double polish method is easy to teach and learn.

A mandibular fracture case of twice internal fixation surgery with poor healing was treated by denture prosthodontics in Affiliated Stomatological Hospital of Nanchang University. The profile of maxillofacial region was improved, the masticatory function was enhanced, and the new bone formation was promoted.
Fracture Fixation, Internal ; Humans ; Mandibular Fractures ; Prosthodontics

Prosthodontic treatment for patient with hypohidrotic ectodermal dysplasia (HED) is difficult to manage because of the loss of occlusal vertical dimension, abnormal alveolar ridge and anodontia A 20-year-old case with HED was reported. The patient was treated with occlusal reconstruction by a maxillary fixed prosthesis and mandibular telescopic prosthesis. At one-year's follow-up, dentures were well used and occlusal function of the patient returned to normal.
Anodontia ; Ectodermal Dysplasia 1, Anhidrotic ; Humans ; Male ; Reconstructive Surgical Procedures ; Young Adult

OBJECTIVETo study the influence of three different metal alloy surfaces treated with primer methacryloxy propyl trimethoxyl silane (gamma-MPS) coupling agent on the bond strength of Filtek Z350 resin.

METHODSTotal 36 specimens of nickel-chromium(Ni-Cr) alloy, pure titanium(Ti) and aurum-palladium(Au-Pd) alloy, each of 12 pieces produced by lost wax casting method, were divided into 6 groups. After treated with gamma-MPS on the bonding surface, experimental group samples were adhered to Filtek Z350 resin, then compared with the surface without gamma-MPS treatment. The tensile and shear strength of each group tested and the fracture sections were observed with scanning electron microscope (SEM).

RESULTSComparing the Ni-Cr alloy and pure Ti experimental group with them in control group, the shearing strength of the experimental group was higher, and the differences were significant (P < 0.001). The shearing strength of the experimental group and control group of Au-Pd alloy were not significant (P > 0.05). Comparing the experimental groups, the shearing strength of pure Ti was higher than Ni-Cr alloy, both were higher than Au-Pd alloy (P < 0.01). These entire specimens were adhesive fracture. Comparing with the control group, Ni-Cr alloy and pure Ti experimental group showed more resin juts in the fracture surface, while the Au-Pd alloy in experimental group showed less resin juts.

CONCLUSIONgamma-MPS can enhance the adhesion between metal and resin, but it is also related to the metal composition. gamma-MPS can significantly increase the bond strength of resin and Ti or Ni-Cr alloy, but not the case of Au-Pd alloy and resin.

Chromium Alloys ; Composite Resins ; Dental Alloys ; Dental Bonding ; Materials Testing ; Shear Strength ; Silanes ; Surface Properties ; Titanium

OBJECTIVETo investigate the color stability of ceromer veneers/resin cements after accelerated ageing, and to provide the reference for clinical application and choice.

METHODSFifteen groups of ceromer veneers/resin cements samples, five samples in each group, were prepared as experimental groups.In the fifteen groups, ceromer veneers in three thickness (1.00, 0.75, 0.50 mm) and resin cements of five shades(A1,A3, B 0.5, WO, TR), were matched through permutation and combination. Three groups of ceromer veneers with different thickness (1.00, 0.75, 0.50 mm) were used as control groups. All samples were put into xenon lamp ageing instrument to accelerate ageing.Spectrophotometer were used to measure the lightness(L(*)), red green color(a(*)) and blue yellow color(b(*)) of samples before and after accelerated ageing process, and the change of lightness (ΔL) , red green color (Δa) , blue yellow color (Δb) and color variation(ΔE) were calculated.We investigated the effect of thickness of ceromer veneer and color of resin cement on color variation by using analysis of variance.

RESULTSThe thickness factor and color factor showed significant effect on ΔE values, and they have interaction (P < 0.05). The ΔE values of all experimental groups were lower than 3.3. After accelerated ageing, all L(*) values of experimental groups decreased, and ΔL values were lower than 2.0.

CONCLUSIONSThe thickness of ceromer veneer and the color of resin cement could both affect the color stability of ceromer veneers/resin cements. The changes of lightness and color in ceromer veneers/resin cements were considered clinically acceptable after accelerated ageing.

Ceramics ; Color ; Composite Resins ; Dental Porcelain ; Dental Veneers ; Humans ; Light ; Resin Cements

Objective : To prepare the La-doped TiO2 film on the surface of 3Y-TZP ceramics in order to observe its antibacterial properties, providing an experimental basis for the application of antibacterial zirconia ceramics in the clinic. Method: A cylindrical 3Y-TZP specimen with a diameter of 20 mm and a height of 3 mm was prepared. The sol-gel method was used to prepare 1% lanthanum (La)-doped TiO2 sol, and the La-TiO2 film-3Y-TZP ceramic was prepared by dip coating on the surface of 3Y-TZP. The TiO2 thin film-3Y-TZP ceramic was prepared by adding no lanthanum nitrate solution in the same way. The surface morphology of 3Y-TZP ceramic specimens (3Y-TZP group), TiO2 thin film-3Y-TZP ceramic specimens (TiO2 film group), and La-TiO2 thin film-3Y-TZP ceramic specimens (La-TiO2 film group) was observed by scanning electron microscopy, and photocatalysis, antibacterial and cytotoxicity experiments were carried out. For the photocatalytic experiment, four specimens were randomly selected from the La-TiO2 film group and TiO2 film group to observe the degradation rate of methylene blue at different time points under sunlight. In the antimicrobial experiment, five specimens were randomly selected from the La-TiO2 film group, TiO2 film group and 3Y-TZP group. The antimicrobial activity of each group was tested using the bacteriostatic circle method, and the bacteriostatic distance of each group was compared. In the cytotoxicity experiment, three samples were randomly selected from the La-TiO2 film group, TiO2 film group and 3Y-TZP group (negative control group), and the extracts were prepared. Here, 0.064% phenol solution were served as the positive control group. Morphological observation of L-929 cells in four groups was performed after 3 d of culture. Cell viability and relative cell proliferation were measured by MTT assay. Results: La-TiO2 films with uniform distribution can be prepared on a 3Y-TZP surface by the dipping-drawing method. Photocatalytic experiments showed that the degradation rate of methylene blue in the La-TiO2 film group was (41.2 ± 1.5)% in daylight for 2 hours, which was higher than that in TiO2 film group (36.5 ± 2.4)%. A significant difference was noted between the two groups (t=3.321, P=0.016). The antimicrobial experiment showed that the antimicrobial distance of La-TiO2 group was (0.34 ± 0.08) mm, which was larger than that of TiO2 group (0.12 ± 0.02) mm. No obvious antimicrobial circle was noted in 3Y-TZP group, and the antimicrobial distance of the La-TiO2 film group was larger than that of TiO2 film group (F=63.798, P < 0.001). Cytotoxicity test showed that the relative proliferation rate of cells in La-TiO2 film group was (89.5 ± 1.3)%, and the cytotoxicity rating was grade 1. Significant differences were noted among the four groups (F=68.250, P < 0.001). Conclusion The La-doped TiO2 film can improve its photocatalytic activity and enhance the antibacterial activity of 3Y-TZP without obvious cytotoxicity.