Objective Optimizing the preparation technology of Red buttock ointment,by exploring the best extraction conditions of the alkannin,radix arnebiae seu lithospermi polysaccharides and so on.Methods Selecting radix arnebiae seu lithospermi 1kg and using orthogonal experiment to explore the best extraction conditions.Naphtho-quinone pigment chemicals were extracted by reflux extraction method;ethanol and paraffin wax were chosen as the composite solvent.Radix arnebiae seu lithospermi polysaccharide was extracted by water decocting,which to observe the rationality of process.Results The best extraction condition is A3 B2 C1 D1 , including section A:ethanol was 20 times the quality of medicinal materials (select radix arnebiae seu lithospermi 1kg and ethanol 20kg),section B:the amount of paraffin was 5% of ethanol(select radix arnebiae seu lithospermi 1kg and paraffin 1 kg),section C:Boiling water was 20 times the quality of medicinal materials,section D:the boiling time was 2h.Conclusion The more practical production process conditions:Ethanol was 10 times the quality of medicinal materials,and the amount of paraffin was 5%of ethanol.Boiling water was 10 times the quality of medicinal materials,and the boiling time was 1 h.This method is economic, simple, reasonable, stable, reproducible and easy to operation.Red buttock ointment obtained by this method possesses of excellent quality,good performance and superior effect.

Objective To assess the value of vascular endothelial growth factor (VEGF) expression in the prognosis of glioma patients.Methods PubMcd,EMbase,Cochrane Library,CNKI and Wanfang data were searched for collecting retrospective cohort studies on the correlation between VEGF expression and prognosis of glioma patients,and relevant articles were also retrieved from inception to June,2016.Two reviewers independently screened the literature,extracted the data,and evaluated the quality.Then Meta analysis was performed with RevMan5.3 software,and the publication bias of literature was evaluated by means of Begg's funnel plot and Egger's method.Results A total of 19 studies with 1657 cases were included.No significant publication bias was found in all groups.The analysis demonstrated positive associations between VEGF expression and poor prognosis (hazard ratio [HR]=1.39,95％CI:1.12-1.73),and the difference was statistically significant (P=0.003).Subgroup analysis showed that the high expression of VEGF in the European and American patients was more valuable than that in the Asian patients in the prognosis of gliomas;large samples of clinical studies of high VEGF expression were more valuable in the prognosis of gliomas than small samples.The detection of VEGF expression by different methods suggested that the patients with high expression of VEGF had poor prognosis;HR provided by the literature suggested that VEGF over-expression was associated with poor prognosis.Conclusion The expression of VEGF has predictive value for the survival time of glioma patients and VEGF can be recommended as a useful survival biomarker in clinical practice.

Objective To study the attention dysfunction of postoperative glioma patients.Methods Attention network test (ANT),digital span (DS),color trail test Ⅱ (CTT Ⅱ) and stroop test (ST) were used to detect the characteristics of attention function of 45 postoperative glioma patients,admitted to our hospital from January 2010 to January 2012 and 18 healthy controls.The relations of attention dysfunction with tumor localization,malignancy and postoperative recovery time were analyzed.Results As compared with that in the control group,orientation network dysfunction was detected in parietal lobe tumor group,with significant difference (P＜0.05); as compared with patients with low grade rumors (grade Ⅰ-Ⅱ),the patients with high grade tumors (grade Ⅲ-Ⅳ) had significantly lower scores in backward recite test,longer time in digital color connection test and higher word color naming interference value,enjoying significant differences (P＜0.05).No significant relation was observed between postoperative recovery time and attention impairment (P＞0.05); Conclusion The frontal and parietal lobe glioma patients suffer attention dysfunction and the high-grade glioma patients have more seriously impairment in attention,attention switching effect and selective attention ability than the low-grade ones.