Objective To explore the protective effect of Radix Astragali (Milkvetch Root,Huangqi)on the retinal ganglion cells (RGC)of high intraocular pressure(HIP)rats,and the influence of HIP on the protective effect.Methods All rats were randomly divided into sham operation group,model group, Huangqi group,eye drops group,and Huangqi + eye drops group.HIP rat model was induced by cauterization of episcleral veins of both eyes.Four weeks after modeling,rats of sham operation group and model group were administered i.g.normal saline,those of Huangqi group were i.g.Huangqi water decoction(20 g/kg),rats of eye drops group took eye drops of Bimatoprost,and Huangqi +eye drops group took i.g.Huangqi water decoction combined with Bimatoprost.After four weeks’intervention,the RGC number and RGC apoptosis rate were analyzed quantitatively using laser confocal microscope. Results The number of RGC in sham operation group was significantly higher than that of other groups (P <0.01),and RGC apoptosis rate was significantly lower (P <0.01 ).While the results of model group was opposite.Although RGC of eye drops group were more than those of Huangqi group,and apop-tosis rate was lower,there were not significant difference(P >0.05).The number of RGC of Huangqi +eye drops group was significantly higher than that of Huangqi group and eye drops group,and the apopto-sis rate was significantly lower than that of the two groups (P <0.05).Conclusion Huangqi water de-coction can protect visual nerves of HIP rats by inhibiting RGC apoptosis,and the protective effects can improve by combination with intraocular pressure lowering medication.

[Objective]To summarize the clinical experience and academic views of Professor YIN Lianrong in the treatment of early retinal degeneration in high myopia.[Methods]Through clinical diagnosis and medical records collection,starting from the pathogenesis and theoretical basis of early retinal degeneration of high myopia,the clinical thought and experience of Professor YIN in the treatment of this disease were summarized,and the experimental case was attached for support.[Results]Retinal degeneration in high myopia progresses slowly,and myopic macular disease seriously impairs vision and is irreversible.Currently,there is no effective treatment.Therefore,Professor YIN combines the thought of"prevention before treatment of disease"with the theory of"eye is able to see with blood"in the clinic,and proposes to move the treatment node forward,that is,to intervene before the occurrence of irreversible macular lesions.Professor YIN believes that the key pathogenesis of the early stage of the disease is Qi and blood deficiency,vein stasis,and the treatment should be closely followed by the method of supplementing Qi and nourishing blood and promoting blood circulation,and flexible use of classical prescriptions Buyang Huanwu Decoction to improve choroidal and retinal blood flow,thereby delaying the progression of fundus lesions,the clinical effect is positive.In the medical case cited,Professor YIN's comprehensive pulse and fundus examination results identified it as the syndrome of Qi and blood deficiency and vein stasis;after treated with supplementation and reduction of Buyang Huanwu Decoction,excellent curative effect was achieved.[Conclusion]Professor YIN combines the idea of"prevention before treatment of disease"with the theory of"eye is able to see with blood",and gives intervention in the early stage of retinal degeneration in high myopia.Through the method of supplementing Qi and activating blood to promote Qi and blood flow and removing blood stasis,the blood flow of choroid and retina is improved,and the disease progression is delayed,the experience is worth clinical learning and reference.