Objective To discuss the clinical technique and outcomes of the treatment with arthroscopically assisted percutaneous retrograde interlocking nailing for supracondylar femoral fractures. Methods From July 2001 to June 2003, there were 22 supracondylar femoral fractures which were 19 fresh fractures and 3 old fractures. There were 10 males and 12 females, and the average age of which was 39.5 years ranging from 22 to 65 years. 7 patients sustained falls, and the other 15 patients were caused by traffic accidents. According to the AO/ASIF classification system, 7 fractures were evaluated as type A1, 8 type A2 and 7 type A3. All the patients were treated by arthroscopically assisted percutaneous retrograde interlocking nailing. Some additional lesions were also treated under arthroscopy simultaneously, including 3 releases for the stiff knee, 2 removals of the loose bodies, 7 debridements and lavages for the osteoarthritis, and 3 bone grafts for the fracture union. Results The average operative time was 130 minutes ranging from 90 to 210 minutes, and the average blood loss was 100 ml. The reduction and fixation were proved to be satisfactory by the postoperative roentgenogram. 20 patients were available for follow-up, which ranged from 6 to 24 months with an average of 14 months. All the fractures were united on an average of 3.3 months postoperatively except one ranging from 2 to 6 months. The one with fracture union were reoperated with bone grafting and compressive plate fixation, and the fracture healed 3 months later. The range of motion was beyond 90? in all patients, no breakage of internal fixation instruments or wound infection occurred. The functional results of knee joint were assessed as excellent in 14, good in 5 and fair in 1, and the rate of excellent and good was 95%. Conclusion The percutaneous retrograde interlocking nailing for the supracondylar femoral fractures is proved to have many advantages, such as less trauma and blood loss, and accurate location, which are beneficial to both of the fracture union and functional recovery.

Objective To verify the ability of distal ends of internal mammary artery and vein used as recipient vessels on breast and chest wall reconstruction. Methods Four bilateral (left 2 and right 2) scent pig buttock island flaps pedicled circle deep iliac artery and vein were used by comparing the different hemodynamic parameters (pressure; flux volume; perfusion unions) between the original pedicl artery (deep iliac artery), proximal and distal ends of internal mammary artery. The free flaps were transplantated by anastomosed end-end to the distal-ends of internal mammary artery and vein after the proximal ends were ligated. Results The pressure of distal end of internal mammary artery was 61%-65% of the pressure with original pedicl artery (deep iliac artery), the immediate volume of anastomasis stoma was a little lower than that of original pedicl artery. The flap, 15 cm?30 cm?2 cm in size, completely survived more than 14 days after operation. Conclusion The slightly decreased distal arterial pressure does not compromise flap survival. Bilateral scent pig buttock island flap pedicled circle deep iliac artery and vein is an ideal animal flap model. This hymodynamic model can be widely used .

Objective To explore the effects of the bio-psycho-social-spiritual health model intervention on the bio-psycho-social-spiritual health model of the patients with cervical cancer.Methods 118 patients with cervical cancer were randomly assigned to the control group (60 cases) and the intervention group (58 cases).All patients received the routing nursing of cervical cancer.In addition the intervention group received the bio-psycho-social-spiritual health model intervention.Functional Assessment of Cancer Therapy-General (FACT-G) was used to investigate their quality of life before being intervened,being intervened for 3 and 6 months.Results The range of FACT-G total score was 44-96 (68.29±11.60).The total scores of FACT-G before the intervention,3 and 6 months after the intervention were 66.82 ±12.21,79.31±5.15 and 89.67±5.21,compared with those of the control group,which were 69.81 ±10.84,74.48±7.97,79.74±7.17,the time effect,Inter group effect and interaction effect were 137.723,10.944 and 14.639,P＜0.01.Conclusions The bio-psycho-social-spiritual health model intervention can improve the quality of life of patients with cervical cancer.

Objective To investigate the relationship between the level of mindfulness and the A-type behavior characteristics of the nursing college students.Methods 172 nursing college students were investigated by the scale of TABP and FFMQ.The results were analyzed.Results In the 172 nursing college students,there were 112 nursing college students belonging to type B behavior pattern,accounted for 65.1％,and 60 nursing college students belonging to type A behavior pattern,accounted for 34.9％.The correlation between the score of TABP and the observation items and describing items were positive statistically significant,and was negatively correlated with awareness items and non-judge items statistically.Conclusions The students with different behavior characteristics were different in their mindfulness level.In order to improve nursing college students' mindfulness level and their mental health,the educators should take intervention based on their behavior characteristics timely.

Objective To analyze data of fails among oncology inpatients from 2014 to 2015,and to explore the effects of the continuous quality improvement on management of falls among oncology inpatients.Methods According to data of falls among oncology inpatients in 2014,continuous quality improvement was carried out in prevention of falls,including health education,risk assessment process of falls and inter-department cooperation.Results One year after applying the continuous quality improvement for management of falls,the number and the rate of falls among oncology inpatients in the year of 2015 (Number=25,Rate=0.027‰) were significantly lower than those in 2014(Number=55,Rate=0.065‰,x2=15.195,P＜0.001).Conclusion Improvement of management protocol for falls can effectively reduce the rate of falls among oncology inpatients and ensure patient safety.

Objective To explore the effects of the mindfulness mode intervention on the adverse reaction of the patients with breast cancer during the period of chemotherapy.Methods 75 patients with breast cancer during the period of chemotherapy were randomly assigned to the control group(38 cases) and the intervention group(37 cases).All patients received the routing nursing of chemotherapy.In addition the intervention group received the mindfulness intervention.The adverse reaction of chemotherapy questionnaire was used to investigate the level of their adverse reaction before the intervention and two months after the intervention.Results After the intervention,the scores of the adverse reaction in the intervention group was (32.19± 7.70)points,less than that of the control group,which was (36.97±10.03)points.There was statistically significant difference between two groups.Conclusions The mindfulness model intervention could reduce the adverse reaction of the patients with breast cancer.

Objective To evaluated the application value of two kinds of mass spectrometer(MS)and Vitek MS system in the i-dentification of routinely isolated bacteria in clinic.Methods 149 strains of common bacteria(including 14 genera and 30 species)i-solated from blood,urine,cerebral spinal fluid,secretion and sputum samples in our hospital from March 2012 to January 2013 were collected and simultaneously identified by 2 kinds of matrix-assisted laser desorption ionization-time of flight mass spectrometer (MALD-TOF-MS).The identification results were compared with those identified by the conventional biochemical identification (Vitek2 compact).The strains with the inconsistent results identified by 3 kinds of method were confirmed by 16S rDNA gene se-quencing.Results Among 149 common bacteria,the correct identification rates of genus and species by the Bruker Biotyper MS were 98% and 96% respectively and which by the Vitek MS system were 97% and 95% respectively.There were no misidentified bacterial strains by these two kinds of MS.Conclusion No statistical difference in the identification results was observed between these two kinds of MS system(P >0.05).Both exhibit excellent identification level and are suitable for the routine laboratory iden-tification of clinical microorganism.

Objective To study the scanning technique of 16-detector multic-slice spiral CT (MSCT) for combined pulmonary artery and deep vein of lower limb in pulmonary thromboembolism (PE) patients.Methods Forty suspected pulmonary thromboembolism patients were performed both pulmonary artery angiography (CTA) and indirect deep vein venography (CTV) on 16-detector MSCT. The parameters of the latter as following :total contrast volume 120-150 ml, injection rate 4.0-4.5 ml/s (from antecubital vein), delay time 4.0 for CTA 20-23 s, CTV 120-180 s, collimation for CTA 1.25 mm and 0.625 mm, CTV 2.5 mm, scan range of CTV: from popliteal vein to the level of bilateral renal vein into the inferior vena cava. Postprocessing include MPR, MIP, and VR. The test was used to analyzed the images.Results Twenty five patients had both pulmonary thromboembolism(PE) and deep vein thromboembolism (DVT), 8 patients had only DVT, 2 had only PE, and 5 had neither. There was no difference between different collimation in depicting thrombus. The CT value number of enhanced pulmonary artery and lower deep vein was obviously higher than the thrombus. The value of MPR, MIP, VR for PE was 100%, 100%, and 65%, The value of MPR,MIP,VR for DVT is 100%, 60%, and 50%.Conclusion The technique of combined pulmonary CTA and deep vein CTV of 16-detector MSCT will provide a new modality for pulmonary thromboembolism patients.

In order to identify rat ovarian germ cells, we expressed and purified rat RVLG protein in Escherichia coli cells and prepared a mouse anti-rat RVLG polyclonal antibody. The rat RVLG cDNA was obtained from rat testicle tissue by RT-PCR and was cloned into the vector pMD19-T. Sequence analysis proves that the cloned RVLG cDNA fragment was 60 bp longer than that released in the GenBank (NM_001077647), resulting from an alternative splicing of the RVLG pre-mRNA. The RVLG cDNA was double digested with the restriction endonucleases BamH I and EcoR I, and then was extracted from gel and inserted into the prokaryotic expression vector pGEX-4T-1. The recombinant expression plasmid pGEX-RVLG was verified for successful construction and then was transformed into Escherichia coli BL21(DE3) for induction to express the GST-RVLG fusion protein by IPTG. The GST-RVLG fusion protein was expressed in Escherichia coli BL21 (DE3) at a high level which accounts for more than 10% of the total bacterial cellular protein. The purified RVLG protein was used as an antigen to immunize KM mouse for the production of polyclonal antibody in ascetic fluid followed by celiacly injecting the mouse with S180 cells. The mouse anti-rat RVLG antibody was analyzed by ELISA, Western blotting and immunohistochemistry for its specificity and titer. The antibody could recognize RVLG protein specifically and its titer was about 1:20 000. These results confirm that the mouse anti-rat RVLG polyclonal antibody with high affinity and specificity has been prepared successfully, and lay a foundation for our ongoing research on the specific expression of RVLG in rat ovary.
Animals ; Antibodies, Monoclonal ; biosynthesis ; Base Sequence ; Cloning, Molecular ; DEAD-box RNA Helicases ; biosynthesis ; genetics ; immunology ; DNA, Complementary ; biosynthesis ; genetics ; Escherichia coli ; genetics ; metabolism ; Female ; Mice ; Molecular Sequence Data ; Ovary ; cytology ; metabolism ; RNA, Messenger ; biosynthesis ; genetics ; Rats ; Recombinant Fusion Proteins ; biosynthesis ; genetics ; immunology

Cell mechanics is essential to cell development and function,and its dynamics evolution reflects the physiological state of cells.Here,we investigate the dynamical mechanical properties of single cells under various drug conditions,and present two mathematical approaches to quantitatively character-izing the cell physiological state.It is demonstrated that the cellular mechanical properties upon the drug action increase over time and tend to saturate,and can be mathematically characterized by a linear time-invariant dynamical model.It is shown that the transition matrices of dynamical cell systems signifi-cantly improve the classification accuracies of the cells under different drug actions.Furthermore,it is revealed that there exists a positive linear correlation between the cytoskeleton density and the cellular mechanical properties,and the physiological state of a cell in terms of its cytoskeleton density can be predicted from its mechanical properties by a linear regression model.This study builds a relationship between the cellular mechanical properties and the cellular physiological state,adding information for evaluating drug efficacy.