USB, with high speed and versatility, has been the standard port between the personal computer and the peripheral equipment. Being difficult to develop, USB has a brilliant future in the field of biomedical engineering. This paper introduces the principle and design method of the function firmware of USB.

With a high speed and high versatility, USB has been the standard port between PC and peripheral equipment. USB can be widely used in Biomedical Engineering, though it is very difficult to develop. This paper is to indicate the principles and design methods of USB application software under Windows and WDM driver for USB device.

Accompanied with the application of CT,MRI and DSA in hospital,the digital radiography is gradually instead of the general radiography.The digital radiography system is more advantage in the field of the image collection,display,procession,management and transmission compared with the general radiography.

OBJECTIVE:To prepare rufloxacin eye drops METHODS:The content of rufloxacin was determined with HPLC method The therapeutic effect of the eye drops was observed in 150 out-patients with severe ocular infections RESULTS:Determined by HPLC method,the average recovery of rufloxacin was 100 12%(RSD=1 18%) After treatment,130 patients recovered,16 improved and had no efficiency with a total effective rate of 97 33% CONCLUSION:The eye drops have definite therapeutic effect The HPLC method is simple,rapid and accurate in determination of the content and no special reagent is needed

Objective: To achieve intelligent access to normal training or individual soldier signs information during the war, establish individual soldier health intelligent interactive regulation and health system platform of IOT, realized digitalization, information management mode. Methods: Using the communication network, intelligent call, vital signs monitoring technology, to design intelligent signs monitoring system. Results:This system is mainly for the automatic sense of individual signs in the training or war, to understand the individual activity, get the sign data, which can send information through a call or intelligent system, based on the identification and location, medical personnel to care and treatment in the shortest time, construction of intelligent information. Conclusion: Through the use of intelligent detection system to Individual life signs in training or in wartime, the system can always grasp of individual soldier signs, and realizing a complete coverage of all of the intelligent health monitoring system, to provide effective guarantee for wartime workers health.

Objective: The mobile medical collaborative service platform can realize mobile nursing informatization, which will bring new changes to the nursing work, and work flow is optimized. The construction of mobile nursing system is the trend of development in informatization, in order to better service to patients, realize full nursing work informatization. Methods: Based on wireless dynamic environment, and three subsystems including nursing collaboration portal, intelligent mobile terminal application system, the message engine service system, it adopts hierarchical architecture design, based on SOA structure mode, whose modules are loosely coupled, and platform core includes: the message engine subsystem, the medical task analysis engine subsystem, WEB middleware subsystem and the client subsystem,in order to realize the service management of mobile medical collaboration. Results: According to the actual situation of nursing work in hospitals, construction of mobile nursing system by the stage of application, to meet the core work of nursing, and then realize the basic nursing work, the construction of digital implementation of nursing information, to realize the informatization construction of digital care. Conclusion: Application of the system to achieve the whole informatization of the nursing management, through access to health services, cloud services center, to share resources, to achieve full coverage of all aspects of smart care management for people's health to provide effective protection.

Mobile X-ray system is a very important equipment in hospital,but it's difficult to protect the user from the exposure of the X-ray while the equipment is in use.This paper introduces a circuit that is able to control the exposure by remote controller and it can solve the problem of the protection better.The efficiency is proved to be good by experiments.

Objective: To design device to measure D/T index which is used for evaluation of arteriosclerosis. Methods:By calculating the detected date of blood pressure values, pulse pressure, pulse rate, pulse patterns and SpO2 on the extremities, we get a set of analytical data in the system in order to analyze the arteriosclerosis situations. Results: The device use the ECG signal as it based and modulated signal, the computer process the date of blood pressure and SpO2, then output the result by the display and printing systems. Conclusion:The device provides an effective noninvasive detection means for extremity vascular.

Objective　To obtain mammalian cell expression vector of human CD154 gene. Methods　A 820 bp cDNA fragment was amplified by RT-PCR method from total RNA of human peripheral blood mononuclear cell（PBMC） activated with 10 ng／mL PMA and 1 μg／mL PHA for 8 hours. The fragment was cloned into pcDNA3.1（＋） plasmids.The cloned insert was identified by double digestion of the recombinant plasmid with restriction enzymes BamH Ⅰ and EcoR Ⅰ and sequenced by Sangers-dideory-mediated chain termination. Results　This cDNA fragment included 820 bp entire coding region and a part of the 3 non-coding region. The recombinant mammalian cell expression vector of pcDNA3.1（＋）／hCD154 was constructed， the sequence of the insert was identical to the published sequence encoding human CD154 antigen. Conclusion　The recombinant mammalian cell expression vector of pcDNA3.1（＋）／hCD154 was successfully constructed.

Objective To explore the efficacy and safety of systemic mild hypothermia in management of neonates with moderate or severe hypoxic-ischemic encephalopathy (HIE).Methods A retrospective case-control study was conducted on 75 neonates with moderate or severe HIE,who were admitted to the Neonatal Intensive Care Unit of Teaching Hospital of Fujian Medical University (Quanzhou Children's Hospital) from January 1,2011 to May 31,2015.The 75 neonates were divided into two groups,the conventional treatment group (33 cases,control group) and the mild hypothermia treatment group (42 cases,hypothermia group).Sequential management protocol for all subjects was followed,including amplitude-integrated electroencephalogram (aEEG) before treatment,aEEG and brain MRI at one week after birth,neonatal behavioral neurological assessment (NBNA) on the 14th day after birth,and determination of mental and psychomotor development index with Bayley Scales of Infant and Toddler Developmental at 18 months old.Adverse reactions,serious disability cases and deaths during the study were also recorded.Two sample-t test and Chi-square test were as statistical methods.Results There were six death cases in the control group,but on one died in the hypothermia group.In the survivals,The maximum voltage and minimum voltage in the hypothermia group were higher at 7-day old than that before treatment [maximum voltage:(31.3 ±2.4) vs (18.1± 2.2) μ V;minimum voltage:(13.5±2.1) vs (6.1 ±1.5) μ V,t=8.591 and 5.314,both P ＜ 0.05],and also higher than that of control group [(25.2±3.1) and (9.3±3.1) μV,respectively,both P ≤ 0.05].Compared with the control group,there were more babies with normal head MRI [43％(18/42) vs 18％(6/33),x2=4.814,P ≤ 0.05] in the hypothermia group at 7-day old and less cases of severe disability [21％(9/42) vs 45％(15/33),x2=4.902,P ≤ 0.05] and deaths [0％(0/42) vs 18％(6/33),x2=6.098,P ≤ 0.05].Higher NBNA score at 14 day and Bayley developmental index at 18 months were shown in the hypothermia group than in the control (39.4±2.6 vs 35.3 ±2.4,t=3.316;mental development index:96.3± 13.2 vs 84.3 ± 10.6,t=7.893;psychomotor development index:98.2 ±16.8 vs 85.4±13.2,t=8.753,all P ≤ 0.05).The adverse effects of hypothermia treatment included electrolyte imbalance (n=12),abnormal blood glucose level (n=8),hepatic and renal dysfunction (n=7),infections (n=6) and bradycardiac (n=4),and no cold injury syndrome case was reported.However,none of the above had significant difference compared with the control group (P ＞ 0.05).Conclusions Systemic mild hypothermia treatment is effective in reducing mortality rate and major disability rate in neonates with moderate or severe HIE and improves the neuromotor development when babies grow up to 18-month-old.