1.Bone marrow hematopoietic microenvironment in patients with systemic lupus erythmatosus
Waiyi ZOU ; Huixia LAN ; Lianqiang FENG ; Hui PENG ; Peida YIN ; Shaokai LUO
Chinese Journal of Pathophysiology 2000;0(10):-
0.05).We did not find any difference of the expression of fibronectin,laminin and type IV collagen in them.Expression of ICAM and VCAM were(56.4?14.8)% and(55.6?12.2)%,respectively,obviously higher than those in control group(P
2.Observation on effect of badu shengji san and its decomposed recipes on morphological changes of injured skin tissues in rats.
Rong HE ; Bo PENG ; Yanli LU ; Jie GAO ; Lianqiang HUI ; Jianrong LI
China Journal of Chinese Materia Medica 2012;37(6):715-718
OBJECTIVETo study the effect of Badu Shengji San (BDSJS) and its decomposed recipes on morphological changes of injured skin in rats.
METHODSD rats with injured skin were treated with BDSJS and its different decomposed recipes for consecutively 14 days. Morphological changes in the injured skin were observed by H&E staining.
RESULTMercury and lead-containing ingredients significantly decreased epidermal thickness and caused vascular hemorrhage, hyperemia and inflammatory cell infiltration in reticular layer of dermis. The compatible herbs alleviated epidermal thickness and reduced dermal lesions.
CONCLUSIONBDSJS' mercury and lead-containing ingredients can accelerate the healing of skin wound and its compatible herbs can relieve the dermis injury induced by mercury and lead.
Animals ; Drugs, Chinese Herbal ; chemistry ; pharmacology ; toxicity ; Epidermis ; drug effects ; injuries ; pathology ; Hemorrhage ; chemically induced ; Hyperemia ; chemically induced ; Lead ; toxicity ; Male ; Mercury ; toxicity ; Random Allocation ; Rats ; Rats, Sprague-Dawley ; Skin ; drug effects ; injuries ; pathology ; Skin Diseases ; drug therapy ; pathology ; Wound Healing ; drug effects
3.Comparison between white light endoscopy and narrow-band imaging endoscopy in predicting histological healing of ulcerative colitis in remission
Tao HE ; Lingyu ZHU ; Peng PAN ; Lei LI ; Qiuye WANG ; Shilin QIU ; Liyan ZHANG ; Hui GAO ; Lianqiang SONG ; Shanming SUN
Chinese Journal of Digestive Endoscopy 2023;40(2):140-145
Objective:To investigate the predictive value of mucosal vascular pattern (MVP) under narrow-band imaging (NBI) enteroscopy in patients with ulcerative colitis (UC) in clinical remission for histological healing and clinical recurrence.Methods:A total of 142 patients with UC in clinical remission who visited the First Affiliated Hospital of Weifang Medical University from January 2018 to January 2021 were included in the study and underwent colonoscopy. The white light and NBI endoscopic images were collected and biopsies were obtained. The Mayo endoscopic score (MES) was calculated based on white light images, and MVP staging was evaluated based on mucosal vascular patterns under NBI. Nancy index (NI) was used to evaluate histological healing and patients were followed up for 1 year. The Spearman correlation coefficients of MES and MVP with histological healing and recurrence were calculated. The receiver operator characteristic (ROC) curve was plotted and the area under curve (AUC) was applied to evaluate the accuracy of white light and NBI endoscopy for predicting histological healing of UC in clinical remission.Results:According to the MVP criteria, 47 were defined as clear, 63 blurred, and 32 invisible. Spearman correlation analysis showed a significant correlation between MVP under NBI and histological healing ( r=0.549, P<0.001) and a moderate correlation between MES under white light and histological healing ( r=0.462, P<0.001). Spearman correlation analysis showed a moderate correlation between MVP under NBI and clinical recurrence ( r=0.451, P<0.001) and a moderate correlation between MES under white light and clinical recurrence ( r=0.352, P<0.001). AUC of NBI for diagnosing histological healing of UC in clinical remission was 0.809 (95% CI: 0.738-0.879), with a sensitivity of 84.6% (77/91) and specificity of 64.7% (33/51), superior to the white light endoscopy, of which AUC, sensitivity and specificity were 0.763 (95% CI: 0.678-0.848), 81.3% (74/91) and 66.7% (34/51). Conclusion:MVP staging under NBI could predict histological healing of UC patients in clinical remission and is superior to white light endoscopy.
4.Evaluation of vitro hepatotoxicity of monocrotaline by precision-cut liver slice technique.
Lianqiang HUI ; Shuangrong GAO ; Ting LIU ; Chunyu CAO ; Jing GUO ; Ran HAO ; Yan YI ; Chunying LI ; Yong ZHAO ; Aihua LIANG
China Journal of Chinese Materia Medica 2011;36(5):628-632
OBJECTIVETo modify the empirical method of precision-cut liver slice technique, and study the hepatotoxicity of monocrotaline by this technique.
METHODLiver slices were prepared by the domestic shaking slicer. The technique of precision-cut liver slice was established by detecting MTT reduction used as the slice viability under different culture medium, thickness of slices, pH and culture temperature. After monocrotaline and liver slices co-culture for 6, 24 h, the slice viability, enzyme activity of GPT, GOT, LDH, GGT and protein concentration were detected by MTT reduction, enzyme kinetics method and BCA protein assay method, respectively.
RESULTWhen the thickness of slices was 200 microm and pH of medium was 6.8, culture temperature was 37 degrees C, BPM culture medium, the viability of slices could maintain on a steady level. LDH leakage was significantly increased and protein content was obviously decreased after monocrotaline co-culture for 24 h with final concentration 0.02, 0.1 and 0.5 g x L(-1). No statistically significant difference between control group and monocrotaline 3 dose groups was observed in the slice viability and the content of GPT, GOT, LDH, GGT and protein after monocrotaline co-culture for 6 h.
CONCLUSIONThe slice viability could retain 24 h in modified BPM medium surroundings; monocrotaline displayed liver toxicity in some degree after co-culture for 24 hours in 0.02, 0.1 and 0.5 g x L(-1) concentration.
Animals ; Hydrogen-Ion Concentration ; L-Lactate Dehydrogenase ; metabolism ; Liver ; drug effects ; metabolism ; Male ; Monocrotaline ; toxicity ; Rats ; Rats, Sprague-Dawley ; Temperature ; Toxicity Tests
5.Development of animal model for anaphylactoid test of rodent.
Chunying LI ; Aihua LIANG ; Shuangrong GAO ; Ting LIU ; Ran HAO ; Chunyu CAO ; Jing GUO ; Yan YI ; Lianqiang HUI
China Journal of Chinese Materia Medica 2011;36(4):488-491
OBJECTIVETo establish a simple and feasible method of anaphylactoid test on awaked small animals for screening and assessing anaphylactoid reaction of traditional Chinese medicine (TCM) injection with different concentration of tween 80.
METHODTest substances containing 0.4% Evans blue were intravenously injected into mice at volume of 20 mL x kg(-1) or guinea pigs at a volume of 30 mL x kg(-1). The behaviors were observed and the vascular permeability of ears evaluated by the extent of ear blue staining and absorbance of Evans blue extraction of ears were tested at 30 min after injection.
RESULTTween 80 solution, Yuxingcao injection with tween 80, and Shuanghuanglian powder injection obviously increased vascular permeability of ears characterized as ear blue staining and increased absorbance of the Evans blue extract from ears extracted by acetone saline both in mice and in guinea pigs in a concentration-dependent (in the case of tween 80) or a dose-dependent (Shuanghuanglian) manner.
CONCLUSIONEar vascular permeability test in mice and guinea pigs can be used as animal models to screen and test anaphylactoid reaction induced by injections.
Anaphylaxis ; chemically induced ; Animals ; Capillary Permeability ; drug effects ; Guinea Pigs ; Male ; Medicine, Chinese Traditional ; adverse effects ; Mice ; Mice, Inbred ICR ; Models, Animal
6.Material and mechanisms induced pseudo allergic reactions of Yuxingcao injection.
Ting LIU ; Yong ZHAO ; Chunyu CAO ; Ran HAO ; Chunying LI ; Yan YI ; Shuangrong GAO ; Lianqiang HUI ; Aihua LIANG
China Journal of Chinese Materia Medica 2010;35(12):1603-1606
OBJECTIVETo investigate the characteristics, sensitizin and the mechanism of pseudo allergic reaction induced by Yuxingcao injection.
METHODBeagle dogs were randomly assigned to control group, 0.5% tween 80 group, Yuxingcao injection without tween 80 group, Yuxingcao injection included 0.5% tween 80 group. The animals in control group were intravenously injected with saline. The other group were intravenously injected with the corresponding test substances. Observe pseudo anaphylaxis of Beagle dogs within 30 min after administration. Blood pressure and respiration rate of Beagle dogs were measured before and after injection drugs 10 min and 30 min respectively. The pseudo allergic reactions were scored at same time points, and the sera of animals were collected to determine the HIS, CH50 and C5b-9 concentration using ELISA.
RESULTThe scores of allergic reaction in 0.5% tween 80 group and Yuxingcao injection included 0.5% tween 80 group was evidently higher than that in control group in 2-5 min after administration. Animals of above two groups showed the symptoms of red swelling on ear part, pruritus, throwing the head, nausea, lapping the tongue, dysphoria and bradykinesia. Some of them had behaved with repose, urination, defecation, cyanosis, the frequency of breathes accelerating and blood pressure decreasing. The rate of pseudo allergic reactions was 100%. Serum CH50 concentration of 0.5% tween 80 group decreased 10 min after injection, while C5b-9 concentration increased. No obvious differences were observed 30 min after injection. There was no significant difference in HIS concentration between control group and treatment groups.
CONCLUSIONThe pseudo allergic reactions appeared after intravenous 0.5% tween 80 and Yuxingcao injection when mixed with tween 80. Furthermore, Yuxingcao injection without tween 80 did not induce pseudo allergic reactions. It was suggested that the pseudo allergic reactions of Yu Xing Cao Injection was related to the cosolvent tween 80. The pseudo allergic reactions of tween 80 may relate to the activation of complement.
Animals ; Disease Models, Animal ; Dogs ; Drug Hypersensitivity ; etiology ; immunology ; Drug Interactions ; Drugs, Chinese Herbal ; administration & dosage ; adverse effects ; Humans ; Male ; Polysorbates ; administration & dosage ; adverse effects ; Random Allocation
7.Detection of bacterial endotoxin content in eight kinds of injection by cytokine revulsion.
Yan YI ; Ting LIU ; Ran HAO ; Jing GUO ; Chunying LI ; Chunyu CAO ; Yong ZHAO ; Shuangrong GAO ; Lianqiang HUI ; Aihua LIANG
China Journal of Chinese Materia Medica 2010;35(15):1965-1969
OBJECTIVEBy using RAW 264.7 macrophage cell line, we studied the dose-effect relationship of endotoxin induced RAW 264.7 cells to release TNF-alpha, and then detected the content of endotoxin in 8 kinds of injections, so that we can investigate the feasibility and the interference factors of the novel test.
METHODBy using endotoxin of different concentrations to induce RAW 264. 7 cells to release TNF-a, we drew the curve of dose-effect relationship between endotoxin and generated TNF-alpha. Then we detected the content of TNF-alpha in yuxingcao, shuanghuanglian, qingkailing, gegensu, xiangdan, qianrongmei and jiangxianmei injections and shuanghuanglian powder injection, and calculated their content of endotoxin.
RESULTThe endotoxin could induce the cells to release TNF-alpha in a good dose-dependent manner, even at a very low concentration. In the range of maximum available dilution multiple, the content of endotoxin in the rest 7 kinds of injections was less than 1.0 EU x mL(-1) except qingkailing injection of two batch.
CONCLUSIONCytokine revulsion has the advantage of wide detection range, high sensitivity, simple operation, and the detected endotoxin is of bioactivity. This method provides another technical mean for pyrogen test of injections.
Animals ; Biological Assay ; methods ; Cell Line ; Drug Contamination ; Drugs, Chinese Herbal ; analysis ; Endotoxins ; adverse effects ; analysis ; Macrophages ; drug effects ; immunology ; Mice ; Tumor Necrosis Factor-alpha ; analysis ; immunology
8.Content detection of bacterial endotoxin in two kinds of injection by gelatin technique.
Shuangrong GAO ; Ting LIU ; Jing GUO ; Yan YI ; Ran HAO ; Chunying LI ; Chunyu CAO ; Yong ZHAO ; Lianqiang HUI ; Aihua LIANG
China Journal of Chinese Materia Medica 2010;35(11):1405-1409
OBJECTIVETo detect content of bacterial endotoxin in Yuxingcao and Qingkailing injections by specific and nonspecific tachypleus amebocyte lysate technique for in order to investigate the feasibility of specific tachypleus amebocyte lysate technique for detecting bacterial endotoxin in traditional Chinese drug injections.
METHODDifferent batches of Yuxingcao and Qingkailing injections were detected by specific and nonspecific tachypleus amebocyte lysate kits.
RESULTYuxingcao injection could be detected by specific and nonspecific tachypleus amebocyte lysate technique, Whereas Qingkailing injection could be detected only by specific tachypleus amebocyte lysate.
CONCLUSIONUsing specific tachypleus amebocyte lysate as a substitute for nonspecific tachypleus amebocyte lysate is an effective method for detecting content of bacterial endotoxin in Qingkailing injection.
Animals ; Drug Contamination ; Drugs, Chinese Herbal ; analysis ; Endotoxins ; analysis ; Horseshoe Crabs ; Limulus Test ; methods
9.Development of gastric precancerous lesion animal model.
Chunying LI ; Aihua LIANG ; Shuangrong GAO ; Lianqiang HUI ; Ting LIU ; Chunyu CAO ; Yong ZHAO ; Ran HAO ; Yan YI ; Jing GUO
China Journal of Chinese Materia Medica 2012;37(1):89-93
OBJECTIVETo establish a model of gastric precancerous lesion by using Aristolochic manshuriensis which contains aristolochic acids.
METHODThe SD rats were randomly divided into four groups: control and three different doses of ethanol extractive of A. manshuriensis (EEA) (corresponding to aristolochic acid I 2.5, 5.0, 10.0 mg x kg(-1)), respectively. EEA was intragastrically given to rats every other day. At the end of the 10th, 15th, 20th week, part of the rats in each group was sacrificed and the stomachs were weighed. The gastric tumor was assessed by the weight and the relative stomach weight to the body weight. The stomachs were fixed in 4% neutral formalin, and the paraffin imbedding tissues were sliced and HE stained. Histomorphology was observed under the light microscope to determine gastric hyperplasia, mucosa precancerosis (atypical hyperplasia) and gastric cancer formation.
RESULTThe rats treated with different doses of EEA for 10 weeks induced mucosa papillary, epithelioma hyperplasia. Histological observation showed mucosa precancerosis lesions characterized as atypical hyperplasia at the dose levels corresponding to aristolochic acid I 5.0 and 10.0 mg x kg(-1) treated for 10 weeks. The incidence rate of gastric precancerosis in those two groups was 100% at the 15th week. Malignant tumors were observed in most of the animals in 10.0 mg x kg(-1) group. The animals in 5.0 mg x kg(-1) group were well tolerant compared to 10.0 mg x kg(-1) group during the course of experiment, so the dose of aristolochic acid I 5.0 mg x kg(-1) and 10-15 weeks treatment were considered to be optimum to establish the model of gastric precancerosis.
CONCLUSIONA rat model of gastric precancerosis can be induced within a short duration by giving an oral administration of the ethanol extract of A. manshuriensis which contains aristolochic acids.
Animals ; Aristolochia ; chemistry ; Aristolochic Acids ; administration & dosage ; Disease Models, Animal ; Drugs, Chinese Herbal ; administration & dosage ; Humans ; Male ; Rats ; Stomach Neoplasms ; drug therapy ; pathology