Objective: To analyze the effect between minimally invasive percutaneous plate fixation and open reduction internal fixation on patients with Tibial shaft fracture. Methods:Chosen patients with tibial shaft fracture as research subjects, randomly divided observed groups receiving minimally invasive percutaneous plate fixation and control group receiving open reduction internal fixation, detecing surgery-related indicators, AKSS knee score and adverse conditions incidence. Results:1)After surgery, surgery time and total hospital stay of observation group patients were significantly shorter than control group patients, blood loss was significantly less than control group patients (P<0.05);2)After surgery, patients AKSS knee function scores were significantly higher than control group patients(P<0.05);3)After surgery, observation group patients’ adverse events incidence was significantly lower than control group patients (P<0.05). Conclusion:Minimally invasive percutaneous plate osteosynthesis can effectively optimize the surgical procedure to improve postoperative joint function and reduce adverse events healing is ideal tibia fracture mode of operation.

AIM:To generate monoclonal antibodies against human augmenter of liver regeneration (rhALR). METHODS:After BALB/C mice were immunized by the purified rhALR, the cells of spleen were fused with the cells of SP2/0; The titer and speciality were respectively fathomed from ascites or foster fluid by ELISA and Western-blot test. RESULTS:2 hybridoma cell lines were successfully obtained. The McAbs titer from ascites and foster fluid are respectively about 10-3-10-5 and 10-2-10-3. It is evident that the two McAbs were directed at different epitopes. CONCLUSIONS:The McAbs have higher speciality. It is significantly useful of the value that how hALR distribute in tissue organs, how the hALR signals the metabolism in the body and the control distribution of the hALR on cell growth on the translational level and so on is researched.