OBJECTIVETo study the effect of seedling quality on growth, yield and quality of Rehmannia glutinosa at harvest and build a basis for its GAP.

METHODThe seedling quality of R. glutinosa in main producing regions was surveyed to understand the current status of seedling quality. Field experiments with different varieties and seedling quality were conducted to measure dry matter accumulation with different growth of R. glutinosa and oligosaccharide content, and economic yield at harvest.

RESULTThe seedling was randomly selected by farmers in R. glutinosa producing regions. Seedling quality could significantly improve on seedling emergence rate, and promote seedling growth, especially with early stage R. glutinosa, finally increase yield at harvest. At harvest, 63% and 50% of yield with A and B seedling could be improved for variety of 85-5, and 50% and 47% of yield could be increased for variety of Beijing No. 1, compared to the C seeding.

CONCLUSIONIn cultivation, the seedlings with the diameter > 1.5 cm should be transplanted firstly.

Oligosaccharides ; analysis ; Rehmannia ; chemistry ; growth & development ; Seedlings ; chemistry ; physiology

Background: Nonsteroidal antiinflammatory drugs (NSAIDs) are widely used in clinical practice. As the progress of endoscopic techniques, NSAIDs-induced small intestinal injury is more frequently to be detected, but there is still lack of effective preventive and therapeutic measures. Aims: To explore the role of regulatory T cells (Treg cells)/Th17 cells imbalance in NSAIDs-induced small intestinal injury and the protective effect of angiotensin 1-7[Ang(1-7)]. Methods: Thirty male Sprague-Dawley rats were randomly divided into control group, model group, and Ang(1-7) treatment group; in the latter two groups, diclofenac sodium was used to induce small intestinal injury. On day 5, the rats were sacrificed to obtain small intestinal mucosa. The macro- and microscopic changes of the intestinal mucosa were evaluated; the levels of Ang(1-7), and pro- and antiinflammatory cytokines were detected by ELISA and/or immunohistochemistry; flow cytometry was used to determine the proportions of Treg and Th17 cells in CD4