Objective To study the state of herpes simplex virus (HSV) infection and the vertical transmission between pregnant women and their fetus/newborns. Methods ELISA was applied to screen HSV specific antibodies in 455 pregnant women. HSV type 2 DNA was detected by PCR technique in the tissues and appendages of fetus, whose mothers had positive HSV IgM and/or type 2 IgG antibodies. Results The infective rate of HSV types1 and 2 during pregnancy were 85.27% and 47.69%, respectively. The intrauterine infective rate was 4.24%. The positive rate of HSV type 2 IgG in the pregnant women with history of abnormal pregnancy was significantly higher than that in the control group. HSV type 2 DNA was found in the amniotic fluid and tissues of placenta and fetus. Conclusion The HSV infection was common during pregnancy. HSV type 2 infection was closely related to the abnormal pregnancy, such as abort, premature delivery and malformation. ELISA combined with PCR technique is a valuable method for the diagnosis of HSV infection.

To further investigate the vertical transmission route of human papillomavirus (HPV) and the indication for the choice of mode of delivery, the infective status of 152 asymptomatic pregnant wemen and the maternal-fetal transmission were studied. By using general primers in polymerase chain reaction (GP-PCR) combined with restriction fragment length polymorphism analysis, HPV DNA positive rate in cervical secretions and venous blood in asymptomatic pregnant women was 36.21% and 52.78%, respectively, and the identified genotypes were mainly HPV16 and 18. The maternal-fetal transmission rate of HPV via genital tract as well as blood was 40.91% and 57. 89%, respectively. It was concluded that besides the transmission route of genital tract and amniotic fluid, there was also transplacental transmission of HPV in utero. Therefore,in our opinion, it is not an absolut indication to perform a cesarean delivery for the pregnant women with HPV asymtomatic genital infection.
DNA, Viral/analysis ; Disease Transmission, Vertical ; Female Urogenital Diseases/*virology ; *Papillomaviridae/isolation & purification ; Papillomavirus Infections/*transmission ; Polymerase Chain Reaction/methods ; Polymorphism, Restriction Fragment Length ; Pregnancy Complications, Infectious/*virology

Summary: To further investigate the vertical transmission route of human papillomavirus (HPV) and the indication for the choice of mode of delivery, the infective status of 152 asymptomatic pregnant wemen and the maternal-fetal transmission were studied. By using general primers in polymerase chain reaction (GP-PCR) combined with restriction fragment length polymorphism analysis, HPV DNA positive rate in cervical secretions and venous blood in asymptomatic pregnant women was 36.21 % and 52.78 %, respectively, and the identified genotypes were mainly HPV16 and 18. The maternal-fetal transmission rate of HPV via genital tract as well as blood was 40.91 % and 57.89 %, respectively. It was concluded that besides the transmission route of genital tract and amniotic fluid, there was also transplacental transmission of HPV in utero. Therefore,in our opinion, it is not an absolut indication to perform a cesarean delivery for the pregnant women with HPV asymtomatic genital infection.

OBJECTIVE: To study the characteristics of perioperative treatment of functional endoscopic nasal sinus surgery in elderly patients. METHOD: Data of 186 patients above 60 years old with chronic sinusitis were retrospectively analyzed. RESULT: After being received systematics and personal individuality treatment, the rates of curing were 72.5%, the rates of improving were 23.6% and the rates of efficiency were 96%. CONCLUSION The treatment of endoscopic sinus surgery is reliable and safe for elderly patients.
Aged ; Aged, 80 and over ; Chronic Disease ; Endoscopy ; Female ; Humans ; Male ; Middle Aged ; Nasal Polyps ; surgery ; Retrospective Studies ; Sinusitis ; surgery ; Treatment Outcome