Objective To study the psychological conditions of HIV/AIDS patients' families.Methods The non-structural interview was done with 4 HIV/AIDS patients' families.The data were analyzed for summarizing the themes.Results Six themes were summarized including suspicion and denial,humiliation and discrimination,helplessness and guilty,fear and sadness, desperate,threat and revenge.Conclusion HIV/AIDS patients’families are under tremendous psychological pressure and economic pressure,and their quality of life is very low.Therefore,it is necessary to lower the negative emotions in HIV/AIDS patients' families so that their quality of life is to be increased.

Objective To explore the effect of continued self-efficacy health education after discharge on patients with benign prostatic hyperplasia. Methods Toally 100 patients with benign prostatic hyperplasia during January to December 2015 were equally assigned to control group and observation group by random digit table:the former was treated with discharge instructions and the latter with discharge instruction plus self-efficacy health education. The two groups were compared before intervention and 6 weeks after intervention in terms of health behavior and self-management ability. Results Before intervention, there were no significant differences between the groups in health behavior and total score of self-management ability as well as the scores on its dimensions (P>0.05). After intervention, however, the total scores on health behavior and self-efficacy ability as well as the scores on the dimensions in the observation group were significantly higher than those of the control group (all P<0.01). Conclusion Post-discharge continued self-efficacy health education can be effective in improving their health behavior and self-management ability, which can promote their recovery.

Objective To study the effect of fish skin collagen peptide on skin collagen metabolism.Methods Twenty-two rots were divided into treatment and control groups.In rats of the treatment group,fish skin collagen peptide at a dose that is 20 times of the recommended dose for human was added to the food for 40 days. Skin HYP was isolated by hydrolysis with hydrochloric acid. The concentration of skin HYP was determined by visible spectrophotometer and converted it to collagen concentration and compared between the 2 groups.Results The skin collagen concentration was significantly higher in the treatment group than that in the control group (69.79%±4.36%;64.36%±4.36%,P＜0.05).At 40 days,the average body weight of rats in the treatment group was (32.1±3.16)g;while it was(36.5± 2.19)g in the control group (P＜0.05).Conclusion Fish skin collagen peptide may help to increase collagen concentration of skin and control body weight.

Objective To develop a HPLC method for psoralenand angelicin content assay in Zhuanggu granules .Meth-ods Agilent Zorbax C18 column(4 .6 mm × 250 mm ,5 μm) was used with methanol and water (45∶55) as mobile phase .The detection wavelength was set at 245 nm .The flow rate was 1 .0 ml/min and column temperature at 25 ℃ .The injection volume was 10 μl .Psoralenand angelicin were extracted with 70% ethanol under water bath .Results The linearity was obtained over 3 .75~40 μg/mlfor psoralenand angelicin .The RSDs were less than 2% .The average recovery was between 94% and 105% . Conclusion This simple and accurate HPLC method was suitable for the quality control of Zhuanggu granules .

Carbapenem-resistant Klebsiella pneumoniae (CRKP) leads to high mortality of infected patients. How to deal with CRKP is an urgent problem in clinical practice, and it is imperative to carry out researchon carbapenem resistance mechanism of CRKP. The two-component systems (TCSs) areassociated with the development of drug resistance in a variety of bacteria, and TCSs were expected to be important therapeutic targets for CRKP. Therefore, this article reviewed the mechanisms of TCSs in the regulation of CRKP from the following several aspects: common mechanisms of carbapenem resistance of CRKP, research progress in drug resistance of TCSs, relationships between Klebsiella pneumoniae and TCSs, and so on. It may provide some research ideas for future research and the references for clinical diagnosis and treatment.

ObjectiveTo explore the mechanism of Si Junzitang in regulating the expression of NKG2A to affect the anti-colon cancer function of natural killer （NK） cells. MethodNK cells isolated from healthy honors were cultured and used to construct the three incubation models of NK cells， human colon cancer HCT116 cells， and NK cells + HCT116 cells （co-incubation）. real-time fluorescence quantitative polymerase chain reaction （Real-time PCR） was conducted to determine the mRNA levels of natural killer group 2 member A （NKG2A） and interleukin （IL）-15 in NK cells， as well as the mRNA level of histocompatibility leucocyte antigen E （HLA-E） in HCT116 cells. The secretion of IL-15 was detected by enzyme-linked immunosorbent assay （ELISA）. Methyl thiazolyl tetrazolium （MTT） assay was employed to determine the applicable concentration of IL-15 and test the effects of Si Junzitang and IL-15 on the activities of NK cells and the HCT116 cells in the co-incubation model. The effects of Si Junzitang and IL-15 on the mRNA levels of NKG2A in NK cells and HLA-E in HCT116 cells were detected by Real-time PCR. Monalizumab （M， anti-NKG2A mab） was used to block the NKG2A-HLA-E pathway in co-incubation model， and then the proliferation of HCT116 cells was detected by MTT assay. ResultThe interaction of NK cells and HCT116 cells up-regulated the mRNA levels of NKG2A in NK cells and HLA-E in HCT116 cells （P<0.05）， as well as the expression level and secretion of IL-15 （P<0.05）. Compared with the blank group， Si Junzitang and Si Junzitang + IL-15 promoted the proliferation and improved the anti-colon cancer function of NK cells （P<0.01）. Furthermore， they down-regulated the mRNA levels of NKG2A in NK cells and HLA-E in the HCT116 cells co-incubated with NK cells （P<0.01）. M and IL-15 + M inhibited the proliferation of HCT116 cells compared with the groups without M （P<0.01）. ConclusionThe interaction of NK cells and HCT116 cells can induce activation of NKG2A-HLA-E pathway to impair NK cell function. Si Junzitang can inhibit the activation of NKG2A-HLA-E pathway to restore the anti-colon cancer function of NK cells.