Orbscan-II anterior system was used for early diagnosis of keratoconus. 48 Eyes of 24 patients with suspicious keratoconus were examined by Orbscan-II anterior system from Dec. 1999 to Dec. 2000 and followed up. The values of Diff and anterior chamber depth (ACD) were recorded. Results indicated that values of Diff and ACD were increased in 4 eyes of 2 patients with keratoconus trend during follow-up. Taking advantage of Orbscan-II anterior system to observe the values of Diff and ACD can early diagnose the sub-clinical keratoconus. The values of Diff and ACD can sensitively report the progression of keratoconus.
Anterior Chamber/pathology ; Cornea/pathology ; Corneal Topography/*instrumentation ; Corneal Topography/methods ; Evaluation Studies ; Keratoconus/*diagnosis ; Keratoconus/ultrasonography

In order to investigate the effect of nerve growth factor (NGF) on the proliferation of rabbit corneal endothelial cells and epithelial cells, the in vitro cultured rabbit corneal endothelial cells and epithelial cells were treated with different concentrations of NGF. MTT assay was used to examine the clonal growth and proliferation of the cells by determining the absorbency values at 570 nm. The results showed that NGF with three concentrations ranging from 5 U/mL to 500 U/mL enhanced the proliferation of rabbit corneal endothelial cells in a concentration-dependent manner. 50 U/mL and 500 U/mL NGF got more increase of proliferation than that of 5 U/mL NGF did. Meanwhile, 50 U/mL and 500 U/mL NGF could promote the proliferation of the rabbit corneal epithelial cells significantly in a concentration-dependent manner. However, 5 U/mL NGF did not enhance the proliferation of epithelial cells. It was suggested that exogenous NGF can stimulate the proliferation of both rabbit corneal endothelial and epithelial cells, but the extent of modulation is different.
Cell Proliferation/*drug effects ; Cells, Cultured ; Dose-Response Relationship, Drug ; Endothelium, Corneal/*cytology ; Epithelium, Corneal/*cytology ; Nerve Growth Factor/*pharmacology

In order to investigate the effect of nerve growth factor (NGF) on the proliferation of rabbit corneal endothelial cells and epithelial cells, the in vitro cultured rabbit corneal endothelial cells and epithelial cells were treated with different concentrations of NGF.MTT assay was used to examine the clonal growth and proliferation of the cells by determining the absorbency values at 570nm. The results showed that NGF with three concentrations ranging from 5 U/mL to 500 U/mL enhanced the proliferation of rabbit corneal endothelial cells in a concentration-dependent manner.50 U/mL and 500 U/mL NGF got more increase of proliferation than that of 5 U/mL NGF did.Meanwhile, 50 U/mL and 500 U/mL NGF could promote the proliferation of the rabbit corneal epithelial cells significantly in a concentration-dependent manner. However, 5 U/mL NGF did not enhance the proliferation of epithelial cells. It was suggested that exogenous NGF can stimulate the proliferation of both rabbit corneal endothelial and epithelial cells, but the extent of modulation is different.

Orbscan-II anterior system was used for early diagnosis of keratoconus. 48 Eyes of 24 patients with suspicious keratoconus were examined by Orbscan-II anterior system from Dec. 1999 to Dec. 2000 and followed up. The values of Diff and anterior chamber depth (ACD) were recorded. Results indicated that values of Diff and ACD were increased in 4 eyes of 2 patients with keratoconus trend during follow-up. Taking advantage of Orbscan-II anterior system to observe the values of Diff and ACD can early diagnose the sub-clinical keratoconus. The values of Diff and ACD can sensitively report the progression of keratoconus.
Adolescent ; Adult ; Anterior Chamber ; pathology ; Cornea ; pathology ; Corneal Topography ; instrumentation ; methods ; Evaluation Studies as Topic ; Female ; Humans ; Keratoconus ; diagnosis ; diagnostic imaging ; Male ; Ultrasonography

Objective To investigate the status and the influencing factors of knowledge, attitude and practice (KAP) towards vision care among parents of preschool children, and provide scientific references for vision care intervention.Methods Totally 821 parents of preschool children aged 5 to 6 from 7 kindergartens in Harbin were selected as the research object by convenience sample method, and they were investigated by self-designed KAP questionnaire towards vision care. Results The total score of parents′ KPA towards vision care was (113.18±9.07) points;the score of knowledge dimension was (12.07±2.03) points; the score of attitude dimension was (47.21±4.24) points; the score of practice dimension was (53.90±6.12) points. The single factor analysis showed that, educational level and family monthly income were influencing factors of vision care knowledge (t=14.366,11.349;P<0.05); family monthly income was the influencing factor of vision care attitude ( t=6.586,P<0.05);educational level, family monthly income and visual impairment of children were influencing factors of vision care practice (t=5.903,8.705,8.264;P<0.05).Conclusions The KAP level towards vision care among parents of preschool children are uneven, and the vision care practice is not ideal. Nursing staff in communities should take targeted measures in vision care education according to different people in different groups, to improve the level of KAP towards vision care.

It introduced diagnostic criteria and epidemic situation of low vision, and analyzed the influencing factors including genetic factors, birth history, eye habit, sleep, outdoor activity, diet and lighting. And it put forward intervention strategies for primary prevention of low vision of preschool children, and called for relevant departments and professionals in the future research and health work to strengthen the concern on visual health of preschool children.