OBJECTIVETo compare gene expression gene profile of nasopharyngeal carcinoma (NPC) tissue with that of normal nasopharyngeal tissues by cDNA array and to discuss possible functions of DNA repair-related genes in NPC tissue.

METHODSAfter hybridization of atlas human cancer cDNA expression array 7742 - 1, atlas hybridization results were analyzed by Atlas Image 1.01 a software package. Using RT-PCR was used to confirm the results.

RESULTSOf 63 differentially expressed genes in quadrangle C including DNA damage response, repair & recombination-related genes, 6 DNA repair-related genes were up-regulated, 12 were down-regulated.

CONCLUSIONDNA repair-related genes may be involved in patho-physiological process of nasopharyngeal carcinoma.

DNA Repair ; genetics ; Gene Expression Profiling ; Gene Expression Regulation, Neoplastic ; Humans ; Nasopharyngeal Neoplasms ; genetics ; pathology ; Nasopharynx ; metabolism ; pathology ; Oligonucleotide Array Sequence Analysis ; methods ; RNA, Neoplasm ; genetics ; Reverse Transcriptase Polymerase Chain Reaction

Objective? To explore the effect of therapeutic touch(TT) combined with progressive muscle relaxation training(PMRT) on reducing perioperative stress response in patients with gastric cancer. Methods? By convenience sampling, 120 patients with gastric cancer admitted in the department of general surgery of a Class Ⅱ Grade A hospital in Jiangsu Province from May to October of 2018 were selected as research objects. According to the random number table, they were divided into the experimental group(n=60) and control group (n=60). Conventional nursing approach was adopted in the control group while TT combined with PMRT was adopted in the experimental group. The Self-rating Anxiety Scale (SAS), Numerical Rating Scale (NRS), and Nursing Satisfaction Questionnaire were used to evaluate the intervention effect. Results? The NRS score of the experimental group were lower than that of the control group at 24 and 48 hours after operation with statistical differences (P＜0.05). After intervention, SAS score of the experimental group was lower than that of the control group, and the difference was statistically significant (P＜ 0.01). At discharge, the nursing satisfaction of the experimental group was higher than that of the control group, and the difference was statistically significant (P＜0.05). Conclusions? The application of TT combined with PMRT is helpful to reduce the stress response in perioperative patients with gastric cancer.