Objective To investigate the dynamic changes of mean platelet volume (MPV) and platelet distribution width (PDW),and to explore the role of MPV and PDW in the prognosis of patients with acute myocardial infarction (AMI).Methods This retrospective cohort study included 312 patients with AMI during 2012 to 2014 in The First Affiliated Hospital of Soochow University.Patients were divided into ST-elevation myocardial infarction (STEMI) group,non ST-elevation myocardial infarction group and low PDW group,high PDW group.Their clinical data and outcomes were analyzed.MPV and PDW were measured successively from admission to day-7 after AMI.The relationship between PDW,MPV and GRACE risk score was further investigated.Results In the STEMI group,the patients were younger (P =0.005),and with higher rates of hyperlipidemia and smoking (P ＜ 0.01).Patients in STEMI group had higher risk of death during hospitalization,compared to NSTEMI (P =0.014).In the high PDW group,the rates of congestion heart failure,cardiogenic shock and Killip ⅣV were higher (P ＜ 0.01;P =0.026;P ＜ 0.01).PDW was significantly associated with mortality of in-hospital,one-year mortality and the risk of re-infarction in one year (r =0.69,P ＜ 0.01;r =0.68,P ＜0.01;r =0.70,P ＜ 0.01).MPV was associated with one-year mortality (r =0.30,P =0.02).Conclusions PDW related to the severity of AMI could predict the risk of in-hospital mortality,one-year mortality and re-infarction.It was helpful to screen out the high-risk patients,so as to make more suitable treatment to improve the prognosis of patients.

OBJECTIVE To provide reference for the optimization of development policy of traditional Chinese medicine. METHODS ROSTCM text mining analysis software was adopted to screen the literature preliminarily ，the PMC policy index model was built ，and the results by PMC index and visual PMC surface were analyzed. RESULTS & CONCLUSIONS A total of 33 policies related to traditional Chinese medicine were included ，and 10 primary variables and 46 secondary variables were set. The average PMC index of 33 policies was 6.15，of which 2 were excellent policies and the rest were good policies. Among the primary variables ，the scores of policy openness ，policy evaluation and policy field were relatively high ，while the scores of release time ，policy level and policy type were low. The analysis of sink index and secondary variables showed that the macro planning of national policies was not specific enough in terms of policy objectives and contents ，with low score ，while the local policies were usually planned in detail. Generally speaking ，there is still much room for improvement in the policies related to the development of traditional Chinese medicine in terms of policy system construction ，government investment ，talent construction ， financing supporting policies ，market access rules ，responsible subjects and legal guarantee.

One major strategy to generate genetically modified mouse models is gene targeting in mouse embryonic stem (ES) cells, which is used to produce gene-targeted mice for wide applications in biomedicine. However, a major bottleneck in this approach is that the robustness of germline transmission of gene-targeted ES cells can be significantly reduced by their genetic and epigenetic instability after long-term culturing, which impairs the efficiency and robustness of mouse model generation. Recently, we have established a new type of pluripotent cells termed extended pluripotent stem (EPS) cells, which have superior developmental potency and robust germline competence compared to conventional mouse ES cells. In this study, we demonstrate that mouse EPS cells well maintain developmental potency and genetic stability after long-term passage. Based on gene targeting in mouse EPS cells, we established a new approach to directly and rapidly generate gene-targeted mouse models through tetraploid complementation, which could be accomplished in approximately 2 months. Importantly, using this approach, we successfully constructed mouse models in which the human interleukin 3 (IL3) or interleukin 6 (IL6) gene was knocked into its corresponding locus in the mouse genome. Our study demonstrates the feasibility of using mouse EPS cells to rapidly generate mouse models by gene targeting, which have great application potential in biomedical research.