Objective To investigate the treatment effects of different fixation for fracture and dislocation of ankle joint and to sum up the experience for clinical treatment.Methods One hundred and fifty patients with fracture and dislocation of ankle joint using 5 different internal fixation surgeries were involved with this research:simple cross Kirschner wire fixation,intramedullary fixation ordinary screws,cancellous lag screw fixation,4 holes common plate fixation,tension band fixation.Patients were followed up and evaluated by the American Orthopedic Foot and Ankle Society Score for scoring,and investigated the postoperative recovery and the occurrence of postoperative infections and complications in patients.Results After followed up from 12 to 24 months with an average follow-up time of 19 months,of 150 patients,there were 29 cases with excellent result,65 cases with good result,44 cases with general and 12 cases with poor result,the excellent and good rate was 62.6％ (94/150).One case with nonunion,6 cases with malunion,7 cases with traumatic arthritis,10 cases with wound pain,the incidence of postoperative complications was 16.0％ (24/150).There were 10 cases with shallow infection,5 cases with deep infection,and 11 cases with severe skin irritation; The incidence of postoperative infection was 17.3％ (26/150).Conclusion Surgical treatment is the main treatment of fracture and dislocation of ankle joint,5 different methods of internal fixation of ankle fractures had good therapeutic effect.

Objective To investigate the protective effect and mechanism of Daucosterol on H2O2-induced oxidative stress in hu-man renal duct epithelial cells(HK-2 cells).Methods HK-2 cells were cultured in vitro,and treated with different concentrations of H2O2(1 μmol/L,5μmol/L,10μmol/L,50μmol/L and 100pmol/L)for 24h in H2O2 treatment group;In Daucosterol intervention group,HK-2 cells were pretreated with 50μmol/L Daucosterol for 2h and then treated with different concentrations of H2O2.CCK-8 assay was used to detect cell viability in each group.Hochest33342/PI fluorescence staining and caspase-3 immunofluorescence assay were used to analyze cell apoptosis.The levels of reactive oxygen species(ROS)were analyzed using DCFH-DA fluorescent probe stai-ning.Western blot assay was used to analyze the expression levels of Bax,Bcl-2 and NOX4.Results The inhibition rates in 50μmol/L and 100μmol/L H2O2 treatment groups were 20.74％±3.32％and 53.90％±2.37％;the inhibition rates in Daucosterol intervention group were 10.29％±1.69％(t=4.859,P=0.008)and 37.38％±3.95％(t=6.212,P=0.003),respectively,and the differ-ences were statistically significant.The results of Hochest33342/PI fluorescence staining showed significantly more Hochest33342 fluores-cence signal aggregation and PI fluorescence positive cells in H2O2 treatment group than in Daucosterol intervention group.Immunofluores-cence assay showed that the expression level of caspase-3 in H2O2 treatment group was significantly higher than that in Daucosterol inter-vention group.ROS level in Daucosterol intervention group was significantly attenuated compared with H2O2 treatment group.The results of Western blot showed that the expression level of NOX4 protein in H2O2 treatment group was higher than that in the control group,but significantly decreased in Daucosterol intervention group;The expression level of Bax protein also decreased in Daucosterol intervention group,compared with H2O2 treatment group.Conclusion Daucosterol intervention can alleviate H2O2-induced oxidative stress in HK-2 cells and reduce apoptosis to exert a protective effect.