1.Establishment of a human large cell lung cancer cell line L9981-nm23-H1.
Liangan CHE ; Qinghua ZHOU ; Yanping WANG ; Jun CHEN ; Lunxu LIU ; Yang QIN ; Zhilin SUN ; Xiaohe CHEN ; Wen ZHU
Chinese Journal of Lung Cancer 2004;7(3):187-190
BACKGROUNDTo establish a human large cell lung cancer cell line L9981-nm23-H1 transfected with wild type nm23-H1 gene.
METHODSpLXSN-nm23-H1-EGFP was constructed by gene clone technique, and L9981-nm23-H1 was established by infected virus of nm23-H1 gene. The DNA and protein expression of nm23-H1 were detected in the transgene large cell lung cancer cell line by PCR and Western blot.
RESULTSpLXSN-nm23-H1-EGFP was constructed successfully, and the nm23-H1 cDNA was inducted to L9981 cell. The protein of nm23-H1 could be detected in L9981-nm23-H1 cell.
CONCLUSIONSProtein of nm23-H1 is stably, continuously and high efficiently expressed in L9981-nm23-H1 cell.
Result Analysis
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