1.The Cytotoxic Effect of ~(103)Pd Particle Radiotherapy Combinded with 41℃ Heating on Human Hepatocarcinoma Cell Line Bel-7402
Liang YE ; Ping CHEN ; Yanmin LIU
Journal of Chinese Physician 2000;0(11):-
Objective To investigate the cytotoxic effect of 103 palladium( 103 Pd) particle radiotherapy combined with 41℃ heating on human hepatocarcinoma cell line Bel-7402. Methods Human hepatocarcinoma cells (Bel-7402) were treated by water-heating and/or 103 Pd particle. The cell growth was determined with MTT assay, and the cell apoptosis was detected by the fluorescent stain of Hoechst33258. Results The cytotoxic effect of 103 Pd radiotherapy combinded with 41℃ heating on Bel-7402 cells was stronger than that of only 103 Pd radiotherapy. Conclusion 103 Pd radiotherapy and 41℃ heating had a synergistic effect on hepatocarcinoma cell Bel-7402, and 103 Pd radiotherapy had the cytotoxic effect on the surviving Bel-7402 cells after hot therapy.
2.Effects of rHu-EPO on myocyte apoptosis and cardiac function following acute myocardial infarction in rats.
Liang, YE ; Xinling, DU ; Jiahong, XIA ; Jiang, PING
Journal of Huazhong University of Science and Technology (Medical Sciences) 2005;25(1):55-8
The mechanisms of rHu-EPO attenuating the apoptosis after myocardial infarction in rats were studied. Thirty-two rats were divided into three groups: sham operation group (Sham), acute myocardial infarction group (MI) and rHu-EPO-treated group (MI+ EPO). Acute myocardial infarction model was made by ligating the anterior descending coronary artery. rHu-EPO was administered i. p. in MI+EPO group at the dose of 5 000 IU/kg body weight immediately after the ligation. Each rat in MI+EPO group received the same dose of rHu-EPO daily the next 6 days. On the 14th day all rats underwent hemodynamic measurements and then killed. The samples were examined with HE stain, immunohistochemistry technique (bcl-2, bax) and TUNEL dyeing. The results showed that hemodynamic function in MI+ EPO group was much better than in MI group. The number of the cells positive for bax and TUNEL in MI+ EPO group was less than that in MI group. The number of the cells positive for bcl-2 in MI+ EPO group was more than that in MI group. These findings suggested that rHu-EPO could treat myocardial infarction by preventing apoptosis and attenuating post-infarction deterioration in hemodynamic function.
3.Investigation of present situation and analysis of positive practice environments of doctors in public hospital in China
Ping ZHANG ; Beizhu YE ; Yufan WANG ; Yi SUN ; Yuan LIANG
Chinese Journal of Health Policy 2017;10(6):38-42
Objective: This study aims to investigate the positive practice environments evaluation of doctors in public hospital and provide suggestions for Chinese health care reform from the perspective of health service providers.Methods: Using self-designed questionnaire, a national cross-sectional survey was performed in 77 public hospitals across seven provinces/ metropolis, involving a total of 3564 doctors.Positive practice environment includes organizational management and doctor-patient two-dimension relationships.Results: The doctor''s mean score of positive practice environment evaluation was 18.02±4.86 (out of 40).Doctors had a higher evaluation (score) of organizational management than doctor-patient relationship.In the organizational management dimension, the personal advice got the lowest scores(2.24±1.04);and in doctor-patient relations, evasion of medical risk got the lowest scores(1.59±0.81).There was a difference in the positive practice scores of physicians in different regions, and the scores of doctors from the western China were the lowest (P<0.001.Doctors from western medicine hospitals had lower evaluation of positive practice environments than ones from Chinese Traditional Medicine hospitals(P=0.002.Conclusion: As per the analysis conducted in this investigation, the current medical practice environment of doctors in China is not a promising one.Doctor-patient relationship is worse than organizational management.Hospitals should adopt a participatory decision-making model in management and establish a broadband performance based compensation to motivate junior doctors.Besides, government should establish reasonable compensation incentive mechanism, with the aim of promoting doctor-patient trust.
4.Chemical constituents from tubers of Cremastra appendiculata.
Liang LIU ; Jing YE ; Ping LI ; Peng-Fei TU
China Journal of Chinese Materia Medica 2014;39(2):250-253
Compounds were isolated through various chromatographic techniques and their structures were identified by spectroscopic data analysis and comparison with those reported in the literatures. Thirteen compounds were obtained from the petroleum ether and ethyl acetate extracts. Their structures were identified as 4,4'-dimethoxy-9,9', 10,10'-tetrahydro-(1, 1'-biphenanthrene) -2, 2', 7, 7'-tetrol (1), 4, 4', 7, 7'-tetrahydroxy -2,2'-dimethoxy-1, 1'-biphenanthrene (2), 3, 5-dihydroxy-2, 4-dimethoxyphenanthrene (3), physcion (4), chrysophanol (5), emodin (6), genkwanin (7), quercetin (8), quercetin 3'-O-beta-D-glucopyranoside (9), 3-methoxy-4-hydroxy phenylethanol (10), syringic acid (11), vanillin (12), and p-hydroxybenzaldehyde (13), respectively. Compounds 1-3 and 5-12 are reported from this genus for the first time.
Drugs, Chinese Herbal
;
chemistry
;
isolation & purification
;
Orchidaceae
;
chemistry
;
Plant Tubers
;
chemistry
5.Inhibitory effect of interleukin-13 on cell proliferation and interleukin-6 production in mesangial cells
Hancheng GUO ; Liming JIANG ; Xiaowen CHEN ; Ping WU ; Feng YE ; Dong LIANG ; Jiaming ZHAO
Chinese Journal of Pathophysiology 1986;0(01):-
AIM: To investigate the effect of interleukin-13 (IL-13) on cell proliferation and interleukin-6 (IL-6) production in mesangial cells. METHODS: Cell proliferation was tested by MTT method. The protein synthesis of IL-6 in mesangial cells was measured by ELISA. The expression of IL-6 mRNA in mesangial cells was evaluated by RT-PCR. RESULTS: IL-13(1 ?g/L-100 ?g/L) inhibited the proliferation of mesangial cell in a dose-dependent manner. Both mRNA and protein of IL-6 in mesangial cells were increased significantly in the presence of LPS and this increase could be reversed by IL-13 (1?g/L-100?g/L). However, this increase could not be reversed by IL-13 if the dose was lower than 0.1?g/L and if the mesangial cells were cultured in 5% FCS RPMI1640. CONCLUSION: IL-13 could inhibit IL-6 expression induced by LPS in mesangial cells . We suggested that IL-13 may be an inhibitory cytokine in the regulation of the mesangial cell proliferaltion and inflammatory reaction in glomerulonephritis.
6.Cloning and prokaryotic expression analysis of squalene synthase 2 (SQS2) from Salvia miltiorrhiza f. alba.
Qi-xian RONG ; Dan JIANG ; Liang-ping ZHA ; Ye SHEN ; Yan ZHANG ; Lu-qi HUANG
China Journal of Chinese Materia Medica 2015;40(7):1259-1265
According to the designed specific primers of gene fragment based on the Salvia miltiorrhiza transcriptome data, a full-length cDNA sequence of SQS2 from S. miltiorrhiza f. alba was cloned by the method of reverse transcription polymerase chain reaction (RT-PCR). The SmSQS2 cDNA sequence was obtained, this sequence is named SmSQS2 and its GenBank registration number is KM244731. The full length of SmSQS2 cDNA was 1245 bp, encoding 414 amino acids including 5'UTR 115 bp and 3'UTR 237 bp. Sequence alignment and phylogenetic analysis demonstrated that SmSQS2 had relative close relationship to the SQS2 of S. miltiorrhiza. The induction of E. coli [pET28-SQS2] in different temperature, induction time, IPTG concentrations and density of inducing host bacterium (A600) were performed, Shaking the culture at 30 degrees C until the A600 is approximately 0.6 and add IPTG to final concentration of 0.2 mmol x L(-1), and then the optimal expression of SmSQS2 recombinant protein were accumulated after the induction time of 20 h. The research provided important base for the study of sterol and terpene biosynthesis of SQS2 in S. miltiorrhiza f. alba.
Cloning, Molecular
;
Farnesyl-Diphosphate Farnesyltransferase
;
chemistry
;
genetics
;
metabolism
;
Models, Molecular
;
Molecular Sequence Data
;
Phylogeny
;
Plant Proteins
;
chemistry
;
genetics
;
metabolism
;
Salvia miltiorrhiza
;
chemistry
;
classification
;
enzymology
;
genetics
;
Sequence Alignment
7.The research of acute kidney injury after coronary artery bypass surgery with or without pump
Fengbo PEI ; Zujun CHEN ; Ping LIU ; Hui WU ; Ye SHI ; Juan DU ; Liang LI
Chinese Journal of Thoracic and Cardiovascular Surgery 2014;30(4):228-230,237
Objective To study the impact of the off-pump coronary artery bypass compared to the on-pump coronary artery bypass technique on the rate of acute kidney injury.Methods A retrospective analysis of 824 patients accepted coronary artery bypass surgery in FuWai Hospital Surgical ICU from March 2011 to January 2013.Patients were divided into two groups according to the surgical approach,597 patients in on-pump group and 227 patients in off-pump group.Then compared the difference of acute kidney injury incidence in the two groups.Results 92 patients in the on-pump group occurred AKI,the incidence of AKI was 15.4%.32 patients in the off-pump group occurred AKI,the incidence of AKI was 14.1%.Two groups of patients had no statistical difference in the incidence of AKI,x2 =0.222,P =0.638.Based on the preoperative baseline creatinine clearance levels.We defined normal creatinine clearance (SCR ≥ 60 ml/min),mild renal insufficiency (45 ml/min < SCR <60 ml/min),moderate renal dysfunction group(31 ml/min < SCR <45 ml/min).Decline with the preoperative creatinine clearance rate,the AKI incidence was elevated significantly,P <0.05.Patients with moderate renal insufficiency,offpump patients had lower incidence of AKI than those in on-pump group,P < 0.05.The incidence of renal replacement in the two group patients had no statistical differences.Conclusion The incidence of AKI after coronary artery bypass surgery had no statistical differences with or without pump.In the patients with preoperative moderate renal insufficiency,off-pump can reduce the incidence of AKI,but could not change the kidney the incidence of renal replacement treatment.
8.Research on Chinese medicine pairs (II)--Their data mining.
Er-Xin SHANG ; Wen-Lin LI ; Liang YE ; Wei ZHOU ; Yu-Ping TANG ; Xin-Sheng FAN
China Journal of Chinese Materia Medica 2013;38(24):4191-4195
Data mining technology has become a powerful tool in traditional Chinese medicine (TCM) research. In this paper, based on the principle and basic requirements of data mining, the mining methods and procedures were described. And then the application of data mining technology in Chinese medicine pair research was classified and summarized, such as the compatibility characters, characteristic pairs, dosage-effect relationship and property compatibility, which provide the direction and data base for modern research of Chinese medicine pair.
Cluster Analysis
;
Data Mining
;
methods
;
Drug Interactions
;
Drug Prescriptions
;
Medicine, Chinese Traditional
;
methods
9.Application of magnetic resonance imaging in the evaluation of microwave ablation for liver cancer
Jingjing PAN ; Jie YU ; Ping LIANG ; Xiaoling YU ; Yan ZHONG ; Haiyi WANG ; Huiyi YE
Chinese Journal of Digestive Surgery 2010;9(1):38-40
Objective To investigate the value of magnetic resonance imaging(MRI)in the evaluation of microwave ablation(MA)for liver cancer.Methods The clinical data of 51 patients with liver cancer who received MA at the General Hospital of PLA from November 2005 to June 2009 were retrospectively analyzed.The MRI findings of 65 nodules before and after the treatment of MA were evaluated by serological examination and needle biopsy of liver.Results All patients received MRI within 1 month after MA.Of all nodules.63 were presented with high signal intensity on T_1-weighted images(T_1 WI)and low signal intensity on T_2-weighted images (T_2 WI)and with peripheral ring-like enhancement.They were diagnosed as complete coagulation necrosis,and the alpha-fetoprotein(AFP)level of the patients decreased from 333.83μg/L before MA to 37.68 μg/L after MA.Two nodules were presented with low signal intensity on T_1 WI and high signal intensity on T_2 WI,and they were diagnosed as local residual.All patients were followed up l month after MA,and 5 nodules showed enhancement with the same image characteristics as local residual.They were diagnosed as local recurrence of liver cancer by needle biopsy of liver and AFP level detection.New intrahepatic nodules in 23 patients and an abdominal nodule in 1 patient were detected with an increase of AFP level(mean,120.16 μg/L).Conclusion MRI Can exactly evaluate the efficacy of MA in the treatment of liver cancer.
10.Therapeutic Observation of Fire Needling plus Chinese Herbal Medicine for Alopecia Areata
Qiulei MA ; Bingjie LIANG ; Peng LI ; Ye ZHAO ; Fanzheng MENG ; Ping LI
Shanghai Journal of Acupuncture and Moxibustion 2015;(3):232-233
Objective To observe the clinical efficacy of fire needling plus Chinese herbal medicine in treating alopecia areata. Method Fifty patients with alopecia areata were randomized into a treatment group of 35 cases and a control group of 15 cases. The treatment group was intervened by fire needling plus Chinese herbal medicine, while the control group was by oral administration of Chinese herbal medicine alone. The clinical efficacies were compared after 3-month treatment.Result The total effective rate was 97.1% in the treatment group versus 73.3% in the control group, and the difference was statistically significant (P<0.05).Conclusion Fire needling plus Chinese herbal medicine is an effective method in treating alopecia areata.