Abstract: Objective　The tuberculosis data of students registered and reported in Xuzhou from 2016 to 2020 were analyzed to provide a basis for Xuzhou to formulate tuberculosis prevention and control measures in schools. Method　The epidemiological characteristics of tuberculosis among students in Xuzhou from 2016 to 2020 were analyzed based on the data from Tuberculosis Information Management System and Xuzhou Statistical Yearbook 2021. Results　From 2016 to 2020, a total of 574 student tuberculosis patients were registered in Xuzhou, with an annual average registration rate of 5.64/100 000. The reported incidence of tuberculosis among students in Xuzhou showed an increasing trend year by year (χ2trend=22.521, P<0.001). There were 365 males and 209 females, respectively, with a sex ratio of 1.75:1. The tuberculosis patients were mainly in the 15-19 years old group and 20-24 years old group. According to the statistical analysis by different levels of education, students from ordinary higher institutions accounted for 48.61% of the total number of confirmed cases, followed by senior high schools and secondary vocational schools, accounting for 20.91% and 14.46%, respectively. Preschool education had the lowest number of confirmed cases, accounting for 1.05%. There was significant difference in the reported incidence of tuberculosis among students at different educational levels (χ2=1 522.39, P<0.001). The highest incidence was reported in ordinary higher institutions, followed by senior high schools and secondary vocational schools, and the lowest incidence was reported in preschool education. Conclusion　Xuzhou city school tuberculosis epidemic integral is on the rise, we should further strengthen tuberculosis control work of school tuberculosis prevention and control measures of implementation in the future, at the same time to strengthen school tuberculosis prevention knowledge propaganda and standardize the schools for disposal of tuberculosis epidemic situation. We should also further strengthen the newborn matriculation examination and students absent from class because of illness monitoring work.

Objective To investigate the effect of curcumin (Cur)on acute lung injury (ALI) induced by one-lung ventilation (OLV)in mice.Methods Sixty C57BL/6J mice were randomly alloca-ted into six groups (n =10):two-lung ventilation group (TLV group),OLV group,OLV+Cur pre-treated with 50 mg/kg group (Cur50 group),100 mg/kg group (Cur100 group),1 50 mg/kggroup (Cur1 50 group)and200 mg/kg group (Cur200 group).Peak and averaged airway pressure (Ppeak and Paw)of mice in each group were recorded.Mice were euthanized and the end of experiment,and left lung tissue was excised.Wet lung weight to dry lung weight (W/D)and total lung water content (TLW)were tested.Pathological changes of lung tissue were observed under light microscope,and changes of ultrastructure of lung tissue were observed by transmission electron microscope.Index of quantitative evaluation for alveolar damage (IQA)and lung injury scores were tested under light mi-croscope.The expression of c-Jun N-terminal kinase (JNK)mRNA and phosphorylated JNK (p-JNK)protein were detected respectively by reverse transcription-polymerase chain reaction (RT-PCR)and Western Blot.Apoptosis index (AI)of lung tissue was determined by terminal dexynucle-otidyl transferase(TdT)-mediated dUTP nick end labeling (TUNEL)method.Results There was no statistical significance at Ppeak and Paw of rats in each group.Compared to TLV group,the expres-sion of JNK mRNA and p-JNK protein were all significantly increased (P <0.05)in OLV group,and W/D,TLW,IQA,lung injury scores and AI were all notably higher (P <0.05);morphological and ultrastructural injuries in lung tissue were notably occured in OLV group.Compared to OLV group,the expression levels of JNK mRNA and p-JNK protein were decreasingly lower (P <0.05 )in the groups of Cur100,Cur1 50 and Cur200,W/D,TLW,IQA,lung injury scores and AI were also de-creased (P <0.05);morphological and ultrastructural injuries in lung tissue were gradually alleviated in the groups of Cur100,Cur1 50 and Cur200.However,there was no statistical significance in the in-dexes mentioned above between Cur50 group and OLV group.Conclusion Cur at doses of 100 to 200 mg/kg has protective effects on lung against OLV injury in mice,which may be related to inhibition of pneumocyte apoptosis induced by JNK.

OBJECTIVE:To observe the clinical efficacy and safety of Enalapril folic acid tablet combined with Shenkang injec-tion in the treatment of hypertensive nephropathy. METHODS:90 patients with hypertensive nephropathy were randomly divided in-to control group and observation group. All patients were given anti-infection,regulating electrolyte balance,supplying amino acids and other conventional treatment. Based on it,control group was orally given Maleic acid enalaprilat folic acid tablet 1 tablet in the morning,once a day;observation group was additionally given 100 ml Shenkang injection adding into 300 ml 10% Glucose injec-tion by intravenous infusion,20-30 drops/min,once a day. The treatment course for both groups was 4 weeks. The clinical effica-cy,and clearance rate of creatinine(Ccr),serum creatinine (Scr),morning urine test urine protein (Up)/urine creatinine (Ucr), serum malondialdehyde(MDA),superoxide dismutase(SOD),total antioxidant capacity(T-AOC)before and after treatment and incidence of adverse reactions in 2 groups were observed. RESULTS:The total effective rate in observation group was significantly higher than control group,the difference was statistically significant(P<0.05). After treatment,the Ccr,SOD and T-AOC in 2 groups were significantly higher than before,and observation group was higher than control group,Scr and MDA were significant-ly lower than before,and observation group was lower than control group,the differences were statistically significant(P<0.05). Morning urine test Up/Ucr,systolic blood pressure and diastolic blood pressure were significantly lower than before,and the differ-ences were statistically significant(P<0.05),however,there was no significant difference between 2 groups(P>0.05). And there was no significant difference in the incidence of adverse reactions(P>0.05). CONCLUSIONS:Based on the conventional treat-ment,Enalapril folic acid tablet combined with Shenkang injection has better efficacy than only Enalapril folic acid tablet in the treatment of hypertensive nephropathy,with similar safety.

[Summary] The pathogenesis of diabetic peripheral neuropathy (DPN ) is the result of complex multiple concurrent pathogenic factors ,among which oxidative stress and inflammation play important roles. Multiple data have indicated that haem oxygenase‐1 (HO‐1) and its degradation products products possess the functions of anti‐oxidation ,anti‐inflammation ,anti‐apoptosis and pro‐angiogenesis ,which now becomes a new target for the treatment of DPN. We will briefly review the progression on the research of HO‐1 and DPN.

Objective] To explore the professor Xie Jingri fromthe theory of liver and spleen in the treatment of chronic atrophic gastritis treatment and medication,in order to further understand the relationship among the liver,spleen and stomach. To broaden the thinking,to improve the clinical curative effect of chronic atrophic gastritis. [Method] With factor liver main spleen transportation as the theoretical basis,from the perspective of the relationship between five zang-organs and six fu-organs,with clinical basis for the case of professor Xie Jingri about the theory of liver and spleen in the treatment of chronic atrophic gastritis is discussed for collection. [Results] Professor Xie Jingri putting forward the theory of liver and spleen in the treatment of chronic atrophic gastritis has profound historical origin,there are documentaries, rules on the use of drugs to people,both focus on the overall,with the evidence-based medicine,and attention to detail,judge of modern,pathogenesis. For chronic atrophic gastritis has opened up a more effective treatment. [Conclusion] Through the elaboration of treatment of chronic atrophic gastritis treatment,medication and typical cases,make the theory of liver and spleen for stomach more clear train of thought,on the theory of liver and spleen theory,improve the efficiency of clinical treatment of chronic atrophic gastritis.

Objective:To investigate the content of interaction and construct the virtuous interaction among the hierarchical medical institutions in rural areas based on the theory of integration and system thinking.Methods:Literature research were applied to analyze the interaction with the use of the CNKI.WanFang and Vip.Results:Interaction was closely related to integration,and the interaction among the hierarchical medical institutions in rural areas mainly included resource interaction,management interaction and service interaction,whose subject was the rural three-level medical institutions.Conclusion:The ultimate goal of virtuous interaction was the quality and safety of the health service.Specific measures of the virtuous interaction included strengthening the construction of health personnel and capital flows,combing the interests of rural three-level medical institutions and integrated management,regionalization of two-way referral and upper-and-lower linkage.

Recent clinical trials suggested that epidermal growth factor receptor (EGFR) targeted agents could benefit many patients with colorectal cancer. This article reviews the clinical efficacy of cetuximab, which is an antibody directed at the EGFR, in the treatment of metastatic colorectal carcinoma. Several articles about EGFR-targeted therapies in metastatic colorectal carcinoma and other solid tumors were reviewed. Cetuximab showed the increase in progression-free survival (3.98 months vs 2.56 months, P

Aim To study the mechanism and inhibitory effect of aspirin on U251 cells in vitro.Methods The effects of aspirin on proliferation of U251 cells were assessed using the MTT assay.Cell cycle analysis was done by flow cytometry.AnnexinⅤ-FITC Apoptosis Detection Kit was used to detect apoptosis of cells.Western blot was employed to analyze expression of apoptosis-related protein Bcl-2 and Caspase-3.Results The growth inhibiton of U251 cells by aspirin was in a time-and-dose-dependent manner.After treatment with 8 mmol?L-1,cell cycle was arrested at G2/M phase.Aspirin also significantly enhanced apoptosis of U251 cells with down-regulated anti-apoptotic protein Bcl-2,and activation of Caspase-3.Conclusions Aspirin can significantly inhibit the growth of U251 cells through inducing cell apoptosis in vitro.

Human promyelocytic leukemia HL - 60 cells labelled with 3H - inositol were treated with vincristine (VCR), it was found that the radioactivity of 3H-phosphatidyl inositol - 4-phosphate (PIP) and 3H - Phosphatidyli-nositol - 4,5 - bisphosphate(PIP2) had no obvious change, but that of 3H-phosphatidyl inositol (PI) decreased rapidly, only accounted for 38. 55%~61.01% of the control during 1~ 120 min of the treatment. It showed that VCRdid not affect the activity of PI kinase and PIP kinase, but enhanced the hydrolysis of PI of HL - 60 cells. The inhibitorty effect of VCR upon cell proliferation and DNA synthesis was observed after above biochemical changes,these results suggest that VCR may inhibit HL-60 cells proliferation by interfering the metabolism of phosphoininositides.

AIM: To observe the effect of heat shock protein 70(HSP70) expression induced by glutamine on Escherichia coli lipopolysaccharides(LPS)-induced vascular hyporeactivity in rats.METHODS: Twenty four healthy male Sprague-Dawley rats were randomly divided into: the control group (n=8); LPS shock group (n=8); glutamine(Gln) treated group (Gln 0.75 g?kg-1 iv, n=8). 6 h after LPS shock, phenylephrine (PE, 0.5-2.5 ?g?kg-1 ) was applied intravenously to all groups and the percentage increase in mean arterial pressure(MAP) was detected, respectively. The concentration-response curves of aorta rings were obtained by cumulative addition of phenylephrine (PE), and PE Emax, EC50 were calculated. The blood concentration of malondialdehyde (MDA), TNF-? and IL-6 were assayed in all groups 30 min and 360 min after LPS shock, respectively. The expressions of HSP70 from heart and aorta were also assayed after 6 h LPS shock.RESULTS: The MAP level induced by PE significantly decreased by 51.4% in LPS shock group compared with the control (P