Abstract: Objective　The tuberculosis data of students registered and reported in Xuzhou from 2016 to 2020 were analyzed to provide a basis for Xuzhou to formulate tuberculosis prevention and control measures in schools. Method　The epidemiological characteristics of tuberculosis among students in Xuzhou from 2016 to 2020 were analyzed based on the data from Tuberculosis Information Management System and Xuzhou Statistical Yearbook 2021. Results　From 2016 to 2020, a total of 574 student tuberculosis patients were registered in Xuzhou, with an annual average registration rate of 5.64/100 000. The reported incidence of tuberculosis among students in Xuzhou showed an increasing trend year by year (χ2trend=22.521, P<0.001). There were 365 males and 209 females, respectively, with a sex ratio of 1.75:1. The tuberculosis patients were mainly in the 15-19 years old group and 20-24 years old group. According to the statistical analysis by different levels of education, students from ordinary higher institutions accounted for 48.61% of the total number of confirmed cases, followed by senior high schools and secondary vocational schools, accounting for 20.91% and 14.46%, respectively. Preschool education had the lowest number of confirmed cases, accounting for 1.05%. There was significant difference in the reported incidence of tuberculosis among students at different educational levels (χ2=1 522.39, P<0.001). The highest incidence was reported in ordinary higher institutions, followed by senior high schools and secondary vocational schools, and the lowest incidence was reported in preschool education. Conclusion　Xuzhou city school tuberculosis epidemic integral is on the rise, we should further strengthen tuberculosis control work of school tuberculosis prevention and control measures of implementation in the future, at the same time to strengthen school tuberculosis prevention knowledge propaganda and standardize the schools for disposal of tuberculosis epidemic situation. We should also further strengthen the newborn matriculation examination and students absent from class because of illness monitoring work.

Stasis is the critical causative factor and pathological production considered in clinic treatment. Promoting the circulation in blood vessel and removing stasis are equally important in treatment. Stasis is interactive with qi and blood. Removing stasis aims to regulating the circulation of qi and blood. Scraping therapy is one of the major approaches to stasis removal in TCM. In clinical practice, the stasis can be removed with scraping, bleeding and cupping, etc. Additionally, removing stasis with scraping should be combined with regulating qi and blood to enhance the therapeutic effect.
Blood Circulation ; Humans ; Medicine in Literature ; Medicine, Chinese Traditional ; methods ; Qi

Baeyer-Villiger monooxygenases (BVMOs) are important biocatalysts to synthesize a series of valuable esters and lactones. Based on protein sequence alignment and crystal structure analysis, a nonconserved hinge which linked NADPH domain and FAD domain was speculated to play an important role in substrate recognition and catalytic oxidation process. Cyclohexanone monooxygenase (CHMO) was selected as a model. Mutants obtained by homologous replacement of the whole hinge almost completely lost its original catalytic activity, demonstrating that the overall hinge structure was of great importance. Some significant sites were identified to greatly affect the catalytic activity and stereoselectivity by alanine scanning mutagenesis, accompanied by enzyme activity assessments and chiral kinetic resolutions. Altering K153 decreased the activity of the enzyme but enhanced the stereoselectivity. Changing L143 site reduced stereoselectivity but had little effect on enzyme activity. Mutation at L144 site dramatically weakened both activity and stereoselectivity. Subsequently, these corresponding sites in phenylacetone monooxygenase were also illustrated to follow a similar rule, revealing a universal importance of these sites in the BVMO family. These results expanded our understanding of the structure-activity relationship of these enzymes and provided more proofs for future directed evolution of BVMOs.
Amino Acid Sequence ; Catalysis ; Mixed Function Oxygenases ; chemistry ; Models, Molecular ; NADP ; Oxidation-Reduction ; Oxygenases ; chemistry ; Protein Conformation ; Sequence Alignment ; Structure-Activity Relationship ; Substrate Specificity

OBJECTIVE:To investigate the utilization of antibiotics in urinary surgery patients in our hospital.METHODS:The problems lie in the perioperative use of antibiotics in a total of 600 patients with ureter stones,kidney stones,bladder stones and(or)benign prostatic hyperplasia(BPH)undergoing urinary surgery from Jan.to June in 2006 and from Jan.to June in 2007 were analyzed in accordance with the Guiding Principles of Clinical Application of Antibacterials and the related regulations of antibiotics in our hospital.RESULTS:The rate of rational use of antibiotics rose from 12.5% before intervention to 88.2% after intervention.The problems of improper selection of antibiotics,irrational timing and too long duration of drug use were improved to a great extent.CONCLUSION:The intervention on the rational perioperative use of antibiotics is effective and it greatly enhanced the rationality in the perioperative use of antibiotics in urinary surgery patients.

There are some problems in the definitions of 'interaction between the heart and the kidney' and 'non-interaction between the heart and the kidney' made in the teaching material of traditional Chinese medicine used by western medicine institutes,colleges and universities.In the teaching material,it explains 'interaction between the heart and the kidney' as the relation between heart fire and kidney water,and 'non-interaction between the beart and the kidney' as the relation between heart-yin and kidney-yin.If we cannot correctly understand the pathogenesis of 'non-interaction between the heart and the kidney',we will fail to determine the concrete treatment method for ' coordinating the heart and the kidney'.Through the analysis and discussion on the theory,clinical syndrome,therapeutic principles and prescriptions for the syndrome of disharmony between the heart and the kidney,the authors draw the conclusion that the therapy for this disease should be determined according to the excess and deficiency of the heat fire and kidney water.For instance,if the deficient kidney water cant ascend to coordinate with the heart and results in exuberance of heart fire,Huanglian Ejiao decoction should be used; in the other situation,if the exuberant heart-fire can't descend to coordinate with the kidney and results in deficiency of kidney yang,Jiaotai Pill should be used.The theoretical results could standardize textbook and give better service to the clinical education.

Facing the current situation of cultivation of humanistic quality of medical students,the affiliated hospital has the necessity and possibility to deeply involve the cultivation of medical students' humanistic quality.Its profound historical and cultural accumulation,a large number of advanced figures and humanistic care system are conducive to the cultivation of humanistic quality of medical students.This paper put forward the establishment of the working group on the cultivation of humanistic quality,the multi-level humanistic curriculum teaching,the motivation of teachers in hospital,the evaluation and assessment of medical students' humanistic quality,and the formation of the combination mode of hospital and medical college in the cultivation of medical student's humanistic quality,are the effective way of strengthening the deep involvement of affiliated hospital in the cultivation of medical students' humanistic quality.

Objective To observe the effect of therapies of activating blood and removing stasis, activating yang and dissipating phlegm, and warming and dredging meridians with aromatic herbs on relieving acute myocardial ischemia (AMI). Methods Rats were randomized into normal group,model group, Xuefu Zhuyu Decoction (XZD) groups (treated with XZD 20.5 and 6.8 gkg-1d-1 ), Gualou Xiebai Banxia Decoction (GXBD) groups (treated with GXBD 18.0 and 6.0gkg-1d-1 ), and Guanxin Suhexiang Capsules (GSC) groups (treated with GSC 0.19 and 0.06 gkg-1d-1 ). The AMI rat models were established by intravenous injection of pituitrin (Pit, 1.5U/kg) through the caudal vein. The effect of the three Chinese medical therapies on AMI rat electrocardiogram (ECG) and plasma endothelin (ET) level was evaluated. Results Compared with the model group, statistical significance was presented in the rise of ST segment of medication groups (P

BACKGROUND:It has become increasingly concerned by the industry to detailed understand the morphology of people’s knee joint, thus achieving personalized and gender differentiated total knee arthroplasty, in order to design the knee joint prosthesis that match the characteristics of Chinese people. OBJECTIVE:To measure and characterize the morphological parameters of distal femur of normal adult Chinese people by CT tomography and three-dimensional reconstruction, and to discuss the differences between male and female in morphology of distal femur. METHODS:Seventy normal Chinese adults (35 males and 35 females, aging from 21 to 60 years) received bilateral knee joint CT scan. The scanning images of distal femur which we needed were col ected and handled with three-dimensional reconstruction or Picasa 3.0 software for col ages and overlapping exposure. The posterior condylar angle, anterior condylar angle and trochlear angle were measured on the images that treated by the software, as wel as the transverse diameter of distal femur, lateral condylar height, and the ratio between them. RESULTS AND CONCLUSION:The mean posterior condylar angle of Chinese women was larger than that of Chinese men (P<0.05);the mean anterior condylar angle of Chinese men was larger than that of Chinese women (P<0.05);The transverse diameter of distal femur, lateral condylar height and the femoral surface ratio (diameter/epicondyle height) of Chinese men were larger than those of Chinese women (P<0.05). The results showed that differences exist in morphology of distal femur between Chinese and western people. Also, the differences exist between male and female in Chinese people. These differences should be concerned in the design of femoral prosthesis for Chinese people as wel as in selecting and placing of femoral prosthesis before or during the total knee arthroplasty.

Human promyelocytic leukemia HL - 60 cells labelled with 3H - inositol were treated with vincristine (VCR), it was found that the radioactivity of 3H-phosphatidyl inositol - 4-phosphate (PIP) and 3H - Phosphatidyli-nositol - 4,5 - bisphosphate(PIP2) had no obvious change, but that of 3H-phosphatidyl inositol (PI) decreased rapidly, only accounted for 38. 55%~61.01% of the control during 1~ 120 min of the treatment. It showed that VCRdid not affect the activity of PI kinase and PIP kinase, but enhanced the hydrolysis of PI of HL - 60 cells. The inhibitorty effect of VCR upon cell proliferation and DNA synthesis was observed after above biochemical changes,these results suggest that VCR may inhibit HL-60 cells proliferation by interfering the metabolism of phosphoininositides.

AIM: To observe the effect of heat shock protein 70(HSP70) expression induced by glutamine on Escherichia coli lipopolysaccharides(LPS)-induced vascular hyporeactivity in rats.METHODS: Twenty four healthy male Sprague-Dawley rats were randomly divided into: the control group (n=8); LPS shock group (n=8); glutamine(Gln) treated group (Gln 0.75 g?kg-1 iv, n=8). 6 h after LPS shock, phenylephrine (PE, 0.5-2.5 ?g?kg-1 ) was applied intravenously to all groups and the percentage increase in mean arterial pressure(MAP) was detected, respectively. The concentration-response curves of aorta rings were obtained by cumulative addition of phenylephrine (PE), and PE Emax, EC50 were calculated. The blood concentration of malondialdehyde (MDA), TNF-? and IL-6 were assayed in all groups 30 min and 360 min after LPS shock, respectively. The expressions of HSP70 from heart and aorta were also assayed after 6 h LPS shock.RESULTS: The MAP level induced by PE significantly decreased by 51.4% in LPS shock group compared with the control (P