AIM:To investigate the effect of Gln deprivation on growth and differentiation of primary APL cells. METHODS: The primary APL cells were collected from 18 cases of APL patients' periphery blood, the patients had not been treated. The cells were incubated in RPMI 1640 without or with different contents of glutamine and with 10% fetal calf sera, at 37℃,5% CO 2 and saturation humidity for 4 days.The initial living cell density was 5?10 8/L. After 4 days incubation,the cells were counted,collected, smeard and stained with Wright-Giemsa, DNA, POX, NAE and NaF inhibition,gulping ink and NBT etc. cytochemical technology. The stained cells were investigated under oil immersion lens. RESULTS: The living cell density became (54.28?4.28)% of the initial in glutamine deprivation group, but the living cell density in control with 4 mmol/L Gln was (108.56?12.27 )% of the initial ( P

Animals ; Anti-Inflammatory Agents ; pharmacology ; Cyclooxygenase 2 ; metabolism ; Inflammation ; metabolism ; Insulin ; pharmacology ; Nitric Oxide Synthase Type II ; metabolism ; RNA, Messenger ; genetics ; Rats

Cardiac Catheterization ; methods ; Heart Defects, Congenital ; therapy ; Humans

Arginine ; therapeutic use ; Burns ; therapy ; Humans ; Nutrition Therapy ; Wound Healing

Burns ; Periodicals as Topic ; Surgery, Plastic

Objective To get a high affinity peptide of vascular endothelial growth factor receptor 3 (VEGFR3) as a potent carrier targeted to lymphangiogenesis of ovarian cancer via the technology of phage display. Methods Solid-phase was panned with direct VEGFR3 extracellular protein coating, then the unbound phage was washed away and the eluted phage was amplified. The positive phage clones were identified by ELISA and sequenced, and the affinity and specialty were identified by competent ELISA. Results After four-round bio-panning, the enriched positive phage clones were identified by ELISA. Eight positive phage clones were sequenced and 5 were consensus (WHGSLKQNLWWY). The short peptide displayed on screened positive phage could bind specifically to VEGFR3, and the binding could be inhibited by natural antibody VEGF-D. Conclusion The phage clone (phage-WHGSLKQNLWWY) obtained via bio-panning of peptide library has a high affinity with VEGF receptor 3. The peptide could be a potent carrier targeted to VEGFR3.

Objective To assess the function of the peptide (WHGSLKQNLWWY) through measuring the a functional affinity constant between humanized VEGF receptor 3 and peptide, then comparing it to that between VEGF D and peptide. Methods After coating the peptide by BSA binding with glutaral couple, the best concentration, best time of peptide to coat the plate and the coating coefficient were determined. With cognizance of the advantages of solid phase method in time consumption and convenience, we assessed the functional affinity constant of engineered peptide and VEGF-D with non-competitive ELISA method. We plotted the D(410) standard curve of the binding reaction of peptide and VEGFR3/Fc using four grades of concentration of VEGFR3/Fc and a series of consistency of peptide. We got the consistency of peptide at the half of maximal OD value through the standard curve and calculated the affinity constant by law of Mass Action. Results The affinity constant of peptide was between 10 7-10 8 mol/L -1, which was only smaller by 10 times than that of VEGF-D. Conclusion The peptide we got from the peptide liberary can strongly bind to the target (VEGFR3), thus providing a theoretical basis for its pharmacal use.

Chemical constituents in the mature fruit peels of Sinocitrus chuana Hort were studied systematically. Five compounds were isolated and identified. They were hesperidin( Ⅰ), nobiletin(Ⅱ) tengeretin(Ⅲ), 4', 5, 7, 8-tetramethoxyflovones(Ⅳ) and synephrine(Ⅴ). Of them the fourth was isolated from this genus for the first time.

Objective To study the clinical and THR?gene mutation of two cases of pituitary thyroid hormone resistance. Methods The clinical data and peripheral blood samples of the patients and their family members were collected, and then DNA was isolated. PCR and direct sequencing techniques were performed to determine if there were mutations in their THR?gene. Results No mutation was found in exon 1-10. Conclusion Pathogenesis of sporadic pituitary resistance to thyroid hormone usually has no relation with mutation of THR?gene.

OBJECTIVE: Cerebral vasospasm (CVS) induced by subarachnoid hemorrhage (SAH) is a difficulty in neurosciences. Because the pathogenesis of CVS remains a matter of discussion, clinical prevention and treatment are currently not sufficient. With the application of advanced experimental technology in recent years, great breakthroughs have been achieved in domestic and foreign studies on potassium channels. Definite mechanism in the region plays a significant role in instruction for clinical prevention and treatment. In this paper, researches related to potassium channels in cerebral vascular vessels after SAH are reviewed.DATA SOURCES: Related articles published between January 1991 and December 2005 were searched by the computer in Medline database with the key words of "potassium channels, subarachnoid hemorrhage, cerebral vasospasm" and the language was limited to English. Meanwhile, relevant articles were also searched in CNKI and Wanfang database with the same key words in Ghinese.STUDY SELECTION: All articles were selected firstly, and literatures related to potassium channel on physiology and pathogenesis of SAH-induced CVS were selected. Researches on biochemistry and gene etc were excluded.DATA EXTRACTION: A total of 45 English articles were collected, among which, 17 met the inclusion criteria. Of 2 Chinese articles collected,one study was in accordance with the inclusion criteria. Eighteen literatures on the pathological and physiological mechanism of CVS after SAH were looked for the full text.DATA SYNTHESIS: Researches showed that remarkable changes occurred in potassium channels in CVS after SAH, while potassium channel modulators had significant interventional effect on CVS in animal experiment by promoting or blocking the ion movements.CONCLUSION: Potassium channels plays an important role in the pathogenesis of SAH-indueed CVS. Some therapeutic intervention may be provided based on the studies of potassium channels in CVS.