[Objective] Discussion about feeding Liuwei dihuang pil to different ages of mental retardation intel igence-type mice, then test their intel igence level and mechanism. [Methods]The changes of ethology expressions are determined in healthy KunMing mice of five groups divided randomly:male mice (n=5), female mice(n=20). Group A:normal control group;Group B:the group with innate kidney deficiency and lead ingested. Group C:the group of lead poisoning, and the offspring mice are administered pil s. Group D:the group with innate kidney deficiency and lead ingested, and the adult mice are adminis-tered pil s. Group E:the group with innate kidney deficiency and lead ingested, and both the adult mice and offspring mice are administered pil s. According to the proportion of male and female mice, make them mate, remove the male mice. Before delivery, do threat means and give 0.1%Lead acetate trihydrate solution to Groups B,C,D,E.Groups D,E have tonifying the kidney. Give routine food to Groups A,B,C. Female mice wil labor in 19~22 days after mating, getting the offspring mice. The offspring mice in different periods of growth are carried out ethology determination and analyzed.[Results]Newborn rat kid-ney stand test shows rat offspring is not better than the success rate of kidney emancipated group, juvenile rat kidney tests show space group behavior test per-formance superior to that of kidney group, and long-term kidney group(offspring, mother on behalf of both rat kidney) or female offspring than only on be-half of kidney groups, multiple comparisons between groups(LSD-t statistical analysis) are statistical y significant, A group compared with B group, C group, D groups respectively(t=27.50,8.10,9.30,3.00,P<0.05), B group compared with C group, D group, E group(t=19.40,18.20,27.80, P al <0.05), C group and D group have difference statistical y significant(t=8.40, P<0.05), D group and E group difference is statistical y significant(t=9.60, P<0.05), young rats tightrope test, kidney group to some extent, is stil better than not Bushen group, multiple comparisons between groups find that:A group and D group, B group and C group, B group and E group, C group and D group, D group and E group have differences statistical y significant(t=22.01, 25.41,20.90,32.11,27.60, P<0.05). [Conclusion]Liuwei Dihuang Pil can improve the intel igence level of mice to a certain extent.

Objective:To explore and evaluate the application of flipped classroom in the integrated curriculum of pathology and pathophysiology overview.Methods:This study included in 172 students from the 8-year program of clinical medicine of Batch 2016 (observation group), and 142 students of Batch 2014 (control group). The observation group adopted traditional classroom teaching combined with flipped classroom teaching, and the control group used only traditional classroom teaching. In the observation group, the teaching contents were redesigned in the form of flipped classroom. The relevant knowledge points were provided before class. Group discussions and in-class presentations were used in the teaching. And the cultivation of students' learning autonomy, interaction and innovation were emphasized. The teaching evaluation was obtained from the questionnaire survey and final exam performance of the two groups of students. SPSS 21.0 was used for t test. Results:Students in the observation group had high satisfaction with flipped classroom which was helpful to improve students' independent learning ability, oral expression ability and innovative thinking ability. However, there was no significant difference in the final exam scores between the two groups ( P=0.306). Conclusion:Flipped classroom teaching can improve students' interest in learning and stimulate their independent learning and innovation. However, the teaching effect evaluated only by the final performance can't reflect the superiority of flipped classroom, which should be assessed by more evaluation indexes.

Adult ; Aged ; Dust ; analysis ; Humans ; Middle Aged ; Niacin ; therapeutic use ; Nitric Oxide ; blood ; Nitric Oxide Synthase ; blood ; Occupational Exposure ; analysis ; prevention & control ; Silicon Dioxide ; adverse effects

Objective To study the differences of chromosomal structure among Yersinia pestis strains isolated from China,and to investigate the reasons of chromosomal rearrangement events occurred in Yersinia pestis as well as the possibility of strain identification and phylogenetic analysis based on the chromosomal rearrangement features.Methods According to the genome sequence data downloaded from web of National Center for Biotechnology Information (http://www.ncbi.nlm.nih.gov/genome),alignment of all the coding sequences (CDSs) among five strains(American strain CO92 as reference and other four completely sequenced strains from Inner Mongolia,Jianchuan of Yunnan,Yulong of Yunnan,Naqu of Tibet in China named 91001,D182038,D106004 and Z176003 as comparison strains) was performed,and then the chromosome of Yersinia pestis was divided into several large DNA segments (named chromosomal plate in the text) according to the similarity of CDSs.Plate arrangement patterns in each strain' s chromosome and gene content of breakpoint regions were determined.Finally,genetic relationships among Yersinia pestis strains were analyzed on the basis of rearrangement diversity from paired-comparison.Results Yersinia pestis chromosomes of strains CO92,D182038,D106004,91001 were composed of 44 relatively independent plates,except strain Z176003.Gene order was very stable within each plate,while it was movable between the plates.Comparing with the reference strain CO92,13 rearrangement events occurred in the chromosomes of both strain D182038 and strain D106004,and 14 rearrangement events involved in Z176003,while 37 rearrangement events occurred in 91001.Paired-comparison data showed that only 8 plates order differences were existed between D106004 and Z176003.Forty-three breakpoint regions were identified on the chromosome of strain CO92,and 39 of them contained insertion sequences,and 25 of them were IS100.Conclusions Yersinia pestis genome represents a high degree of genetic flux,and chromosomal structures of strains are significantly different from each other.Chromosomal rearrangement events is closely related to the large number of insertion sequences in the Yersinia pestis chromosome.Rearrangement diversity among Yersinia pestis strains could reflect their genetic relationships.

AIM:To investigate the effect of Epstain-Barr virus latent membrane protein 1 (EBV-LMP1) on proliferation and cell cycle of nasopharyngeal carcinoma (NPC) cells. METHODS:The expression of EBV-LMP1 was detected by immunohistochemical method (LSAB). Proliferation of NPC cells was identified by MTT method. Cell cycle percentage was detected by flow cytometry analysis. RESULTS: OD value of EBV-LMP1 expressive NPC cells L-CEN1 was much higher than that of both EBV-LMP1 negative NPC cells V-CEN1 and CNE1 ( P 0.05).CONCLUSION:The expression of EBV-LMP1 on NPC cell might cause some change of cell cycle and enhance cell proliferation.

Objective To investigate the distribution of two single nucleotide polymorphism(SNP)-717A/G and-390C/A/T-within the C reactive protein(CRP)promoter region in ethnic Han Chinese and the association with coronary heart disease(CHD).Methods Altogether positive CHD patients and negative controls were studies in this study.The two target fragments were amplied by PCR.Then the-717A/G polymorphism was screened by restriction fragment length polymorphism(RFLP)and the-390C/A/T polymorphism by Sequence Specific PCR(SSP).At last,the association was studied by proper statistical analysis.Results There was no significant difference in the distribution of-717A/G polymorphism betweenpatients and controls.However,the frequencies of allels in-390C/A/T polymorphismwere significant different between them(P

Extraction of teeth is the most basic and widely used operation of oral and maxillofacial surgery. According to characteristics of extraction of teeth, the author views medical ethics principles which in the diagnoses and treatments process from the aspect of medical ethics, they are,①Doctors must intensify their own quality and self - cultivations and establish the idea of " People First ". ②Doctors must carry on the overall and systematic inspection, make the plan of diagnoses and treatments synthetically toimplement the idea of " People First ". ③Doctors should pay attention to interact with patient, meet different patients' demands and create the harmonious medical environment.

Objective To investigate the expression and clinicopathological significance of CXCR4 and HIF-1α protein in esophageal squamous cell carcinoma tissue and explore their correlation.Methods The expression of CXCR4 and HIF-1α protein were assessed by immunohistochemistry SP method in 56 cases with esophageal squamous cell carcinoma and in 20 cases with surrounded normal tissue.Results The expressions of CXCR4 and HIF-lα in esophageal squamous cell carcinoma were significantly higher than those in normal tissues[62.50 ％ (35/56) vs 10.00 ％ (2/20); 57.14 ％ (32/56) vs 0(0/20)](x2=16.259,19.740,P ＜0.01).The expression of CXCR4 and HIF-1α were both correlated with invasion depth (x2 =4.736,7.665,P ＜0.05) and lymph node metastasis ( x2 =7.207,6.389,P ＜0.05),and had no correlation with cancer cell differentiation.The expressions of CXCR4 and HIF-lα in esophageal squamous cell carcinoma were positively correlated (r =0.298,P ＜0.05).Conclusion CXCR4 and HIF-1α are highly expressed in esophageal squamous cell carcinoma.The two have a close relation to esophageal squamous cell carcinoma growth,invasion and metastasis.

Objective: To observe the impact of vareniline tartrate on vascular endothelial function and inlfammatory factor releasing in acute coronary syndrome (ACS) patients with nicotine dependence after smoking withdrawal treatment.
Methods: We recruited the in-hospital ACS patients who were smoking ≥10 cigarettes/day for more than 10 years with at least moderate nicotine dependence, and randomly divided them into 2 groups: Varenicline group, the patients received oral medication for 2 weeks and Self withdrawal group, the patients without medication assistance.n=52 in each group. All patients received (10-30) min daily mission and consulting for quit smoking for 2 weeks. The basic information was recorded and blood levels of NO, IL-6 and ET-1 were compared before and after withdrawal treatment.
Results: Compared with they were before, after 2 weeks withdrawal treatment, in Varenicline group, blood levels of ET-1 decreased as (33.950 ± 1.439) ng/L vs (170.198 ± 12.602) ng/L and IL-6 decreased as (0.103 ± 0.020) ng/L vs (0.307 ± 0.051) ng/L; in Self withdrawal group, ET-1 decreased as (60.795 ±7 .036) ng/L vs (170.511 ± 12.374) ng/L, all P<0.05; while NO levels were similar,P>0.05. After treatment, ET-1 level in Varenicline group (33.950 ± 1.439) ng/L was lower than Self withdrawal group (60.795 ± 7.036) ng/L and IL-6 level in Varenicline group (0.103 ± 0.020) ng/L was also lower than Self withdrawal group (0.258 ± 0.042) ng/L, allP<0.05; while NO levels were similar between 2 groups,P>0.05.
Conclusion: Compared with self withdrawal, varenicline tartrate may effectively inhibit inlfammatory factor releasing in ACS patients with nicotine dependence, and therefore improve the vascular endothelial function.

Objective To explore the role of ethyl pyruvate (EP) on E-cadherin of airway epithelium and airway inflammation in a TDI-induced mouse asthma model. Methods 30 male BALB/c mice were randomly divided into control group , asthma group and EP group. On day 1 and 8 , mice in asthma group and EP group were treated with 0.3%TDI on the dorsum of both ears for sensitization. And on day 15 , 18 and 21 the mice underwent an aerosol inhalation of 3% TDI, and saline (100 mg/kg) was injected intraperitoneally 1 hour before inhalation. The control group underwent acetone and olive oil (AOO) sensitization on day 1 and 8, AOO challenge on day 15, 18 and 21. Saline (100 mg/kg) was injected intraperitoneally 1 hour before challenge. One hour before each challenge, mice were given EP (100mg/kg) or vehicle via intraperitoneal injection. On day 22, airway reactivity, IL-4 , IFN-γand IgE in the serum were detected , immunohistochemistry and WB were used to assess E-cadherin levels. Results Airway reactivity, IL-4, IFN-γin and IgE in the serum in asthma group are significantly higher than that in control group (P<0.05). Treatment with EP dramatically decreased airway hyperresponsiveness in TDI-challenged mice, as well as IL-4, IFN-γ and IgE (P < 0.05). E-cadherin in control group was distributed evenly at the connection of epithelial cells. E-cadherinin distribution was chaotic and its expression was decreased in asthma group. EP intervention can ameliorate the damage of E-cadherinin. Conclusions EP can ameliorate the destruction of E-cadherin in airway epithilum by TDI.