The nature and functions of astrocytes (AS)， the pathological changes and roles of AS after spinal cord injury， the experimental methods of inhibiting AS proliferation and glial scar formation， and so on, were reviewed. AS beneficially affects repairing injured spinal cord at different periods of differentiation. However， adult AS secreting the factors formed the chemical glial barrier which severely affects nerve regeneration and hinders axon extension. Because of the static， activated and proliferative AS tending to coexist after spinal cord injury， and complex factors of barrier formation， the current approach taken by a single method is difficult to effectively control the AS proliferation and glial scar formation.

Objective To compare the effect of hemodiafiltration(HDF) on the incidence rate of intradialytie hypotension. Methods We followed up eighteen patients with symptomatic hypotension history and divided them into group A and group B.Group A was treated with conventional hemodialysis(CHD) and HDF, Group B adopted HDF with low temperture hemodialysis. The number of hypotensive episodes, blood temperature during cardiopulmonary bypass, energy transfer rates were assessed. Results The incidence of symptomatic hypotension of patients was found lower in CHD (40%) than in HDF (6%),P<0.01. Energy transfer rates were founded higher in CHD than in HDF and low temperture hemodialysis, P<0.01. Conclusions HDF significantly reduced hemodialysis-related side effects and increased the tolerance ability of patients.

Currently ,EphB receptor has been demonstrated as an important factor on the biological be -havior of normal cells .Some studies found that it plays an important role in the regulation of normal cell morpholo -gy,cell adhesion ,migration and angiogenesis .There is a certain relationship between abnormal expression of EphB receptor with a variety of tumor development .Visiblely,EphB receptor is important research direction in cancer studies,understanding the relationship between EphB receptor and tumor can better realize the progress of the tumor,and further to provide more scientific basis for cancer treatment and prevention .

Objective To study the clinical efficacy of colorectal mucosa anastomosis in resection of mid-lower rectal cancer to preserve the anus. Methods A retrospective analysis was carried out with 208 cases of mid-lower rectal cancer operated with the said surgical procedure, including 131 males and 77 females, aged 21-99 years with mean age of 53.7 years. The low margins of the tumors were located 6-8 cm from the anal verge. Pathologically, adenocarcinoma accounted for 201 cases, in whom 86 well-differentiated, 100 moderately differentiated, and 8 poorly differentiated, 7 mucinous adenocarcinoma, and 7 adenoma with malignant degeneration. According to Dukes classification, 76 belonged to Dukes′ A, 122 Dukes′ B, and 10 Dukes′ C. Results 182 cases (87.5%) were followed up, the median follow-up time was 5.9 years. Postoperative complications included 8 cases (3.4%) of stoma leak, and 3 cases (1.7%) of stoma stenosis. The defecation function recovered after 12-24 weeks postoperatively, with frequency of 1-3 times each day. Local recurrence was 4.9% (9/182). Hepatic metastasis was 14.2% (26/182). The five year survival rate was 72.5%. Conclusion Colorectal mucosa anastomosis is safe and efficacious for rectal cancer. With the procedure, the anal function can be preserved well, the incidence of stoma leak is decreased, and the 5 year survival rate is the same as that of Miles operation.

BACKGROUND:Demineralized bone can be used as the scaffolds of osteoblasts,and body tissue developed immunologic rejection to demineralized bone.OBJECTIVE:To investigate the histological changes of cyclosporin A(CSA) effect on ossification of demineralized bone,in addition,to explore the feasibility of demineralized bone served as ideal available bone substitute material.DESIGN,TIME AND SETTING:The randomized control experiment of animals was performed at the Experimental Center of Second Hospital of Dalian Medical University between March 2007 and April 2008.MATERIALS:Sixteen New Zealand rabbits were divided into the control and experimental group of homogenic decalcified bone,control and experimental group of heterogenous decalcified bone.CSA was produced by Beijing Novartis Pharmaceutical Co.,Ltd.METHODS:Decalcified bone prepared from rabbits was served as homogenic decalcified bone,and decalcified bone prepared from dogs were served as heterogenous decalcified bone.Sixteen rabbits were randomly divided into 4 groups:the control and experimental group of homogenic decalcified bone,control and experimental group of heterogenous decalcified bone,with 4 rabbits in each group.Each rabbit was implanted with homogenic or heterogenous decalcified bone,respectively,2 samples per side.2 mg/kg CSA or 2 mg/kg placebo was intramuscular injected in the experimental or control groups for 4 weeks.Samples were examined by hematoxylin-eosin staining and light microscope.MAIN OUTCOME MEASURES:The histological observation of decalcified bone in each group.RESULTS:Homogenic allogeneic bone induced formation in all implants.CSA did not induce morphological changes of homogenic allogeneic bone.In the heterogenous decalcified bone treated group,at 4 weeks,there was no bone formation or chondrocytes production in the control group,but there was cartilage and bone formation in the experimental group.CONCLUSION:CSA did not alter the morphology of bone induction by homogenic allogeneic bone.Immunologic reactions may inhibit bone induction by heterogenous decalcified bone,which can be counteracted by treatment with CSA.CSA can increase the rate of nonunion or bone defect using heterogenous decalcified bone.

Objective To investigate the methylation status of E-cadherin(E-cad)and its protein expression in human small bowel adenocarcinomas(SBAC),and the association of these findings with clinico-pathological characteristics.Methods E-cad gene hypermethylation and its protein expression in 36 patients with primary SBAC and the paired adjacent tissues were tested by methylation-specific PCR(MSP)assay and immunohistochemistry(IHC)respectively.Data associated with clinico-pathological characteristics was analyzed.Results The hypermethylation frequency of E-cad was 38.9% in cancerous tissues and 8.3% in adjacent tissues(P＜0.01).The positive rate of E-cad expression was 41.7% in cancerous tissues and 97.2% in adjacent tissues(P＜0.01).Twelve out of 21 cases without protein expression revealed methylated(P＜0.01).Methylation status was closely related to E-cad protein expression in SBAC.The hypermethylation of E-cad gene and the expression Of E-cad protein were significantly correlated with the depth of invasion,the differentiation and lymph node metastasis(P＜0.05),but were not correlated with other clinico-pathologic parameters such as age,sex,tumor size or location(P＞0.05).Conclusions The hypermethylation of E-cad gene can down regulate the protein expression,which closely related to the depth of invasion,the differentiation and lymph node metastasis.

Objective To evaluate the clinical effects in treating periprosthetic femoral fractures classified Vancouver B2 with a cementless modular revision femoral prosthesis.Methods Twenty-six patients (7 males and 19 females with mean age of 66.5 years,range 60-69 years) who suffered from periprosthetic femoral fractures classified Vancouver B2 were treated with a cementless modular revision femoral prosthesis in hip revision and open reduction and internal fixation with cables or plates from March 2007 to February 2014.The previous femoral prostheses included 21 cementless and 5 cemented hip stems.The period from former procedures to fractures was 16 months,range from 7 to 32 months.Harris hip scores and numerical rating scales were analyzed statistically.Time of leaving bed,discharging,fracture union postoperative complications and radiographic imaging were also recorded.Results The operation duration were 143 (range 105-165) minutes.Blood loss volume was 450 (range 270-780) ml.Blood transfusion volume were 330 (range 0-800) ml.All patients were followed up at least two years,averagely 3.5 years after surgeries.All fractures healed at 6.5 (range 3-12) months averagely after surgeries.Harris hip scores improved significantly from 11.8±2.5 before surgeries to 80.3±5.3 one month and to 91.4±7.3 six months after surgeries separately (F=0.715,P=0.001).Numerical rating scales were 9.6±0.3,9.4±0.4 and 9.1±0.7 points averagely in aspect of information acquisition,postoperative pain control and function recovery respectively one month after surgeries.Average time of leaving bed and discharging from surgeries was 68.3 (range 51-75) hours and 6.5 (range 5-9) days respectively.No postoperative complications was found except dislocation in 1 hip,lower limb discrepancy in 1 hip,DVT in 1 hip,and heterotopic ossification in 3 hips.Conclusion Due to achieving prosthetic stability and fracture union,periprosthetic femoral fractures classified Vancouver B2 treated with a cementless modular revision femoral prosthesis combined with open reduction and internal fixation with cables or plates can reach good clinical outcomes.

Objective To investigate the effects of methylprednisolone (MP) on the recovery of postoperative neurological functions due to cervical spondylotic myelopathy.Methods 114 patients with cervical spondylotic myelopathy were divided into the MP group and the control group with 57 cases in each group. The cases of the MP group were treated with MP (20 mg/kg, iv) 30 min prior to the decompression. Those of the control group underwent anterior decompression only. The complications of patients in two groups were compared. The neurological functions of all patients were graded according the ASIA score system preoperatively and at 1st day, 2nd week and 6th month postoperatively.Results Compared with the control group, the neurological functions of the cases in the MP group improved significantly after operation ( P＜0.05).Conclusion For patients with cervical spondylotic myelopathy, MP used during operation can improve postoperative neurological function recovery.

Targeting the key questions in the scale development process of TCM syndrome, such as the definition of the concept, the construction of the theoretical framework, the quantitative classification of the items, the rational use of the statistical methods and so on, this article put forward that on the basis of carefully distinguishing the three concepts of syndrome diagnosis, syndrome evaluation and disease diagnosis, and based on TCM dialectical thinking and mathematical validation to build theoretical framework. A scientific and reasonable quantitative classification method was established based on the reliability and validity as indexes. Non-linear intelligent mathematical statistics and symptomatic index groups were used to analyze the ideas and methods of data mining, with a purpose to improve and perfect the methodology of the development of syndromes scale and to improve the establishment and application of the syndrome scale.

?AlM: To explore the effects of the prescription of reinforcing kidney, nourishing blood, improving eyesight on the oxidative stress model of ARPE-19 cells induced by acrolein.
?METHODS:SD rats serum containing the prescription of reinforcing kidney, nourishing blood, improving eyesight and the content of distilled water in serum were prepared. The effects of the prescription and distilled water in serum at different concentration ( 2. 5%, 5%, 10%, 20% and 40%) on cell vitality was observed by cell counting kit ( CCK-8 ) assay. the logarithmic phase of ARPE-19 cells were pretreated by different concentrations (1. 25%, 2. 5%and 5%) of the prescription serum and distilled water in serum for 24h. Then it was treated with 75μmol/L acrolein for 24h. Cell vitality was observed by CCK-8 assay. The change of cell nucleus was detected by DAPl staining .
?RESULTS: 2. 5% and 5% serum had no effect on cell viability (P>0. 05), while 10%, 20%, 40% serum could inhibit cell viability (P<0. 05). CCK-8 results showed that 2. 5% and 5% the prescription of reinforcing kidney, nourishing blood, improving eyesight serum was better than distilled water in serum (P<0. 05).
?CONCLUSlON: The prescription of reinforcing kidney, nourishing blood, improving eyesight has the protective effect on ARPE-19 cell damage induced by acrolein.