OBJECTIVE:To optimize the formulation parameters of poly1,3-bis(p-carboxyphenoxy) propane-sebacic acid (P(CPP∶SA)) microsphere and evaluate its drug release performance in vitro. METHODS: The paclitaxel microspheres were prepared by single emulsion method,and the effects of the factors such as the stirring speed (A),P(CPP∶SA) concentration (B),emulsifier polyvinyl alcohol (PVA) concentration (C) on encapsulation efficiency were evaluated by orthogonal test. The surface morphology of the prepared microsphere was observed and its drug release performance in vitro was evaluated. RESULTS: The optimal formulation of the microsphere obtained was as follows: A was 4 000 r?min-1,B was 80 mg?mL-1 and C was 1%,respectively. The morphology of microspheres was round and intact. The encapsulation efficiency of paclitaxel was up to above 90%. The sustained release duration was 30 days with an accumulative release rate of over 80%. CONCLUSION: Paclitaxel microspheres prepared by optimal formula display a high encapsulation efficiency and satisfactory sustained-release pattern.

In recent years,scientific reports say that ApoE genotypes not only influence lipid metabolism but also affect bone metabolism through vitamin K. Primary osteoporosis is mainly regulated by genes and the interaction of genes and environmental factors also affects primary osteoporosis. This review focused on the mechanism of the influence of ApoE gene on bone mineral density and interaction of ApoE gene and environment factors in the determination of primary osteoporosis.

Acupuncture Therapy ; Female ; Humans ; Middle Aged ; Neuromyelitis Optica ; physiopathology ; therapy ; Vision, Ocular

Hospital Information System(HIS) contributes a lot to the management of the hospital with the personnel flow,the material flow,the fund flow and the information flow well-regulated.The construction of HIS has to be based on general scheme,and then the sub-systems are established separately,at last all sub-systems are integrated to form a system,that is HIS,to realize the modernization of the hospital management.

This study explores the association between physician-patient communication and healthcare service quality,and relevant factors.The physician-patient communication belongs to medical behaviors,and plays a key role in the improvement of physician-patient relationship and the overall healthcare service quality.The consults of physician-patient communication were affected by many factors including physicians' attitude,and usage of words and behaviors,in which the positive attitude and behaviors are key solution to the improvement physician-patient relationship.Therefore the urgent matters are to strengthen standardized management of physician-patient communication,and set up related regulations and effective measure.

Objective:To explore the influence of chaperone tapasin on HLA E expression on cell surface.Methods:The infection method was used to transfer the recombinant retrovirus expression vector which was constructed with HLA E molecules on target cells was detected with FACS technique.Results:Exogenous HLA E expression on T2 cell surface was detected with 74.13% and no HLA E expression was detected on control cells and that was transfected with empty vector.Conclusion:It is possible that the expression of HLA E molecules on cell surface involves in TAP independent mechanism.

A case of mandible plasma cell myeloma is reported.Plasma cell myeloma is a monoclonal neoplastic proliferation of plasma cells of bone-marrow derivation,usually multi-centric but jaw bones are rarely involved.The treatment requires precise pathological diagno-sis.The prognosis of solitary jaw bone lesions can be good after treatment.

Objective To investigate the CT features of ovarian cystadenofibroma(CFA)and to improve the diagnostic accuracy. Methods The clinical data and CT images of 7 patients with ovarian CFA were retrospectively analyzed.All cases underwent non-enhanced and contrast-enhanced CT scan.Results Ovarian lesions were unilateral in 5 cases,bilateral in 2 cases and totally 9 lesions were found.6 lesions were cystic,2 lesions were mainly solid and 1 lesion was mixed components.The maximum diameter of lesions ranged from 4.7-21.5 cm with an average of 8.2 cm.Lesions had smooth margins,and solid portions had different size vesicles. Cyst walls or septa showed various degree of thickening with calcification in 1 lesion and mural nodules in 5 lesions,and a small vesicle was visualized in only 1 of the mural nodules.Cystic portions appeared as fluid density without enhancement.Solid portions,thickened cyst walls and septa appeared as slight enhancement or no enhancement.No regional lymph node metastasis was found,no ascites or peritoneal implants as well.Conclusion Ovarian CFA has some characteristic of CT findings that may improve clinical diagnosis and differential diagnosis.

OBJECTIVE:To develop an HPLC method for simultaneous determination of calycosin-7-O-?-D-glycoside,ono-nin,calycosin and formononetin in Radix Astragali from eleven different habitats and to explore the internal factors of geoherbalism based on aspect of contents of active constituents. METHODS:The sample was separated on Zorbax Eclipse XDB-C18(250 mm?4.6 mm,5 ?m)column. The mobile phase consisted of acetonitrile-0.2% formic acid solution (gradient elution). The UV detection wavelength was set at 280 nm. RESULTS:The linear ranges of calycosin-7-O-?-D-glycoside,ononin,calycosin and formononetin were 0.005 1～0.510 mg?mL-1,0.005 0～0.300 mg?mL-1,0.004 9～0.294 mg?mL-1,0.004 6～0.276 mg?mL-1,respectively(r=0.999 9).Calycosin-7-O-?-D-glycoside and ononin took up a big proportion in Radix Astragali from Inner Mongolia and Shanxi; calycosin and formononetin took up a big proportion in Radix Astragali from Anguo,Chicheng of Hebei province and Dingxi of Gansu province. CONCLUSION:The method is simple,rapid and accurate. Results of study are in line with textual research on Radix Astragali.

Th17 cells have recently emerged as a third independent helper T(Th) cell subset that is different from the classical Th1 and Th2 cells.Th17 cells can specifically secrete IL-17 and play an important role in tumorigenesis and metastasis.In this article, we review the origin and differentiation regulation of Th17 cells, the relationship between IL-17 and tumor microenvironment, angiogenesis and metastasis, and try to provide a new direction for the diagnosis and treatment of tumor.