Acupuncture Therapy ; Female ; Humans ; Middle Aged ; Neuromyelitis Optica ; physiopathology ; therapy ; Vision, Ocular

In recent years,scientific reports say that ApoE genotypes not only influence lipid metabolism but also affect bone metabolism through vitamin K. Primary osteoporosis is mainly regulated by genes and the interaction of genes and environmental factors also affects primary osteoporosis. This review focused on the mechanism of the influence of ApoE gene on bone mineral density and interaction of ApoE gene and environment factors in the determination of primary osteoporosis.

OBJECTIVE:To optimize the formulation parameters of poly1,3-bis(p-carboxyphenoxy) propane-sebacic acid (P(CPP∶SA)) microsphere and evaluate its drug release performance in vitro. METHODS: The paclitaxel microspheres were prepared by single emulsion method,and the effects of the factors such as the stirring speed (A),P(CPP∶SA) concentration (B),emulsifier polyvinyl alcohol (PVA) concentration (C) on encapsulation efficiency were evaluated by orthogonal test. The surface morphology of the prepared microsphere was observed and its drug release performance in vitro was evaluated. RESULTS: The optimal formulation of the microsphere obtained was as follows: A was 4 000 r?min-1,B was 80 mg?mL-1 and C was 1%,respectively. The morphology of microspheres was round and intact. The encapsulation efficiency of paclitaxel was up to above 90%. The sustained release duration was 30 days with an accumulative release rate of over 80%. CONCLUSION: Paclitaxel microspheres prepared by optimal formula display a high encapsulation efficiency and satisfactory sustained-release pattern.

Hospital Information System(HIS) contributes a lot to the management of the hospital with the personnel flow,the material flow,the fund flow and the information flow well-regulated.The construction of HIS has to be based on general scheme,and then the sub-systems are established separately,at last all sub-systems are integrated to form a system,that is HIS,to realize the modernization of the hospital management.

This study explores the association between physician-patient communication and healthcare service quality,and relevant factors.The physician-patient communication belongs to medical behaviors,and plays a key role in the improvement of physician-patient relationship and the overall healthcare service quality.The consults of physician-patient communication were affected by many factors including physicians' attitude,and usage of words and behaviors,in which the positive attitude and behaviors are key solution to the improvement physician-patient relationship.Therefore the urgent matters are to strengthen standardized management of physician-patient communication,and set up related regulations and effective measure.

Objective To detect the methylation status of CDH1 3 gene promoter in colon cancer by using methylation-specific polymerase chain reaction(MSP)technique.Method The tissue specimens from 32 cases of colon cancer(observation group),and 12 cases of normal colon tissues(control group)were examined,the methylation of CDH13 gene promoter was detected by MSP.Result The methylation of CDH13 gene promoter was detected in 19 cases(59.4％,19/32)in observation group,1 case(8.3 ％,1 / 12)in control group,there was statistical significance between two groups(P =0.002).Conclusion Frequency of the methylation of CDH13 gene promoter is apparently higher in colon cancer tissues than that in normal colon tissues,it reveals that CDH13 gene promoter may contribute significantly to the development of colon cancer.

Objective To investigate the CT features of ovarian cystadenofibroma(CFA)and to improve the diagnostic accuracy. Methods The clinical data and CT images of 7 patients with ovarian CFA were retrospectively analyzed.All cases underwent non-enhanced and contrast-enhanced CT scan.Results Ovarian lesions were unilateral in 5 cases,bilateral in 2 cases and totally 9 lesions were found.6 lesions were cystic,2 lesions were mainly solid and 1 lesion was mixed components.The maximum diameter of lesions ranged from 4.7-21.5 cm with an average of 8.2 cm.Lesions had smooth margins,and solid portions had different size vesicles. Cyst walls or septa showed various degree of thickening with calcification in 1 lesion and mural nodules in 5 lesions,and a small vesicle was visualized in only 1 of the mural nodules.Cystic portions appeared as fluid density without enhancement.Solid portions,thickened cyst walls and septa appeared as slight enhancement or no enhancement.No regional lymph node metastasis was found,no ascites or peritoneal implants as well.Conclusion Ovarian CFA has some characteristic of CT findings that may improve clinical diagnosis and differential diagnosis.

Objective:To explore the influence of chaperone tapasin on HLA E expression on cell surface.Methods:The infection method was used to transfer the recombinant retrovirus expression vector which was constructed with HLA E molecules on target cells was detected with FACS technique.Results:Exogenous HLA E expression on T2 cell surface was detected with 74.13% and no HLA E expression was detected on control cells and that was transfected with empty vector.Conclusion:It is possible that the expression of HLA E molecules on cell surface involves in TAP independent mechanism.

Objective: To observe the influence of circumferential pulmonary vein isolation (CPVI) on P-wave polarity and amplitude and to investigate whether the changes of P-wave are associated with patient outcomes after initial CPVI. Methods Fifty patients with paroxysmal atrial fibrillation (AF) underwent CPVI. For each patient, electrocardiograph (ECG) recordings were taken before and 7 days after ablation. The P-wave polarities and amplitudes were analyzed. Successful CPVI was defined as freedom of symptomatic and asymptomatic AF at the end of two consecutive follow-ups. Results: The amplitude of positive P-wave was significantly decreased in the leads of I, II, III, aVF, V5, and V6 after ablation (P<0.05). The amplitude of negative P-wave was significantly decreased in the leads of aVR and aVF after ablation (P<0.05). The total amplitude of P-wave was significantly decreased in the leads of I, II, aVR, V5 and V6 after ablation (P<0.05). The negative P-wave in the lead of III and the positive P-wave in the lead of aVL were increased after ablation. The amplitudes of negative, positive and total P-waves before and after ablation were not significantly different between successful CPVI (n=39) and failure CPVI (n=11) groups. Conclusion: CPVI can result in noticeable reduction of P-wave amplitude in several leads of ECG, indicating the reduction of left atrial electric capacity. The changes of P-wave polarity indicate a P-wave vector shift. The reduction degree of P-wave amplitude has no predictive value for the success or failure of CPVI.

Objective: To characterize the anatomic morphology of pulmonary vein ostium by 2-D and 3-D computed tomography angiography. Methods: Thirty patients with paroxysmal/persistent atrial fibrillation (AF) undergoing computed tomography angiography before catheter ablation of AF were analyzed for the anatomic morphology of pulmonary vein ostium in 2-D and 3-D manner. Results: The diameter in axial, coronal and sagittal views of diameters of the pulmonary veins ostium (PVs) were (16.90±4.79) mm, (21.37±4.23) mm, (22.41±3.96) mm in the left superior pulmonary vein(LSPV), (13.50±3.99) mm, (15.84±3.22) mm, (16.82±3.63) mm in the left inferior pulmonary vein(LIPV),(17.77±4.69) mm, (19.11±4.10) mm, (19.71±4.33) mm in the right superior pulmonary vein(RSPV),and (15.33±3.88) mm, (16.20±4.00) mm, (17.10± 4.24) mm in the right inferior pulmonary vein(RIPV). The maximal and minimal diameters (dmax and dmin) of PVs in 3-D view were (24.30±4.54) mm, (17.76±4.24) mm in LSPV, (19.10±4.45) mm, (12.27±3.52) mm in LIPV, (22.99±5.04) mm, (16.19±4.87) mm in RSPV, and (18.63±4.60) mm, (14.46±3.48) mm in RIPV. There is difference of diameters between SPVs and IPVs (P<0.01) in two-dimensional and three-dimensional view except for RIPV. Conclusion: CT imaging can present precise 3-D reconstruction of the pulmonary vein ostium, allowing for understanding the details before catheter ablation. There is great variance in the dimension of the pulmonary vein ostium among individuals, which should be taken into consideration before operation.