Objective:To explore the significance of combined fibrinogen concentration and neutrophil-lymphocyte ratio as a prognostic marker of colorectal cancer.Methods:A retrospective analysis of the hospital in June 2010-June 2011 of received and accepted the surgical treatment of colorectal cancer patients,respectively analysis the relationship NLR and FIB with the pathological characteristics of colorectal cancer,neutrophils and lymphocytes ratio (NLR) and the concentration of fibrinogen (FIB) were combined to form a index (FIB-NLR).250 patients with colorectal cancer recording the scores were divided into three groups,patients NLR acuity 2.95 and FIB≥348 mg/dl as FIB-NLR 2 groups,NLR acuity 2.95 and FIB < 348 mg/dl or NLR < 2.95 and FIB acuity 348 mg/dl for 1 group,NLR < 2.95 and FIB < 348 mg/dl of 0 group,and analyzed three groups with invasion depth,clinical staging and lymph node metastasis of colorectal cancer,nerve invasion,distant metastasis and histological grades.And the three groups respectively for survival analysis,and carried on the comparison to the survival rates of three groups.Results:Moderate and advanced and with lymph node metastasis of colorectal cancer patients was significantly higher than the earlier stage and no lymph node metastasis in patients with NLR,statistically significant difference (P<0.001);tumor infiltration depth deeper,nerve invasion and distant metastasis of the NLR value was significantly higher in patients with infiltrating depth shallow,without nerve invasion and distant metastasis in patients with NLR values,the difference was statistically significant (P=0.006,P=0.002,P=0.007).Moderate and advanced stage,lymph node metastasis and distant metastasis of colorectal cancer patients with the FIB values were significantly higher than the early and without lymph node metastasis and distant metastasis of colorectal cancer patients FIB values,the difference was statistically significant (P<0.001),the deeper the infiltration depth and colorectal cancer patients with neural infiltrates FIB values significantly higher than the infiltration and shallow depth,of FIB values for patients with nerve infiltration,the difference was statistically significant (P=0.015,P=0.012).NLR and FIB in tumor histologic stage,age,gender,tumor location,had no significant association (P>0.05). The patients with later clinical stage of colorectal cancer,the deeper the infiltration depth,lymph node metastasis,distant metastasis had a high FIB-NLR score than infiltration and shallow depth,without lymph node metastasis and distant metastasis,no nerve infiltration patients was high,the difference was statistically significant (P<0.001).Survival analysis found that the higher the score of patients the 5-year survival rate was lower,the difference had statistical significance (P=0.001).Conclusion:Neutrophil-lymphocytes ratio (NLR) and fibrinogen (FIB) joint form a index FIB-NLR may be a potential progression effective index which estimate the progression of colorectal cancer.

ObjectiveTo evaluate the effect of electroencephalography(EEG) recognition rate resulted by stimulus of visual, auditory and combined perception. MethodsIn virtual traffic environment, the stimuli were designed in traffic information based on visual, auditory and audio-visual fusion. When one of the three stimuli appeared, the subjects completed starting and braking vehicle by imaginary using of right and left hands at the same time. The EEG signals were recorded and the imaginary motion-related features from C3, C4 were extracted and classified. ResultsThe best recognition rates in visual, auditory and fusion perception were 100％, 100％and 83％, respectively, while the averages of the rates were 68.8％ 、82.2％、76.9％, respectively. Conclusion The recognition rates are affected by the audio, visual and fusion stimulus and apparent individual differences exist.

ObjectiveTo define the high intraarterial signals on conventional MRI. Methods Review the conventional MRI data in all of the acute ischemic stroke patients in the First Affiliated Hospital,School of Medicine, Zhejiang University from January 2010 to December 2010. ResultsIn year 2010,according to the diagnostic criteria of China, there were 280 cases of acute cerebral infarction, all underwent conventional MRI. Twenty six patients (9. 29％ ) showed high intraarterial signals on conventional MRI.There were 14 cases with high signals in the anterior circulation, including 11 cases in the carotid artery, 2 cases in the anterior cerebral artery, and 1 case in the middle cerebral artery combined with the anterior cerebral artery. In the 11 patients with high signals in the carotid artery, MRA confirmed 14 internal carotid arteries stenosis, with 100％ specificity and 78.6％ sensitivity. There were 12 cases with high signals in the posterior circulation, including 5 cases in the vertebral artery, 3 cases in the basilar artery, and 4 cases in the basilar artery with vertebral artery. One patient did not undergo MRA. MRA confirmed 14 arteries with stenosis, with 100％ specificity and 78.5％ sensitivity. ConclusionsIntravascular high signals showed by conventional MR sequences have high clinical specificity, and its vascular lesions are consistent with the results of MRA.

Objective To investigate the characteristic of etiology of chronic cough in Shenzhen.Methods The chronic cough etiology was analyzed in 136 cases with the guidance of cough diagnosis and treatment guidelines(2009 editions)published by Chinese Medical Association.The cough was the main or sole symptom,the duration was no less than 8 weeks and chest X-ray film was normal.Results The causes of chronic cough was confirmed in 125 patients and was not definitely diagnosed in 11 patients by inspection and treatment.Cough due to single cause was found in 104 patients(83.20%,104/125),due to compound causes was found in 21 patients(16.80%,21/125).The first 4 etiologies were cough variant asthma(CVA)with 57 patients(36.31%,57/157),upper airway cough syndrome(UACS)with 41 patients(26.11%,41/157),eosinophilic bronchitis(EB)with 17 patients(10.83%,17/157),occupational injury(including harmful,toxic substances inhalation,etc.)with 10 patients(6.37%,10/157).Conclusions The most common cause of chronic cough in Shenzhen is CVA,UACS,EB.Due to the developed industrialization,there is a lack of understanding the cough course of inhaling more harmful and toxic gases and substances in the manufacturing process.So this should be paid more attention.

Objective To evaluate the value of 18F-FDG PET/CT in assessing radiosensitivity enhancement by irisquinone (IR) on rabbit xenografted VX2 lung tumor models.Methods Twenty-four tumor-beating rabbits were randomly divided into 3 groups (8 rabbits/group):group A with radiotherapy alone,group B with combined radiotherapy and IR,and group C without radiotherapy (the control group).18F-FDG PET/CT imaging was performed before radiotherapy and 24 h and one week after radiotherapy.The tumor SUVmax on delayed imaging was calculated in all rabbits.Two rabbits in each group were sacrificed after PET/CT imaging.HE staining was used to assess the differences in cancer cells among groups.Paired t test,one-way analysis of variance and Kaplan-Meier analysis were performed to analyze the data using SPSS 13.0.Results Before radiotherapy,the tumor SUVmax of all the 24 rabbits on standard and delayed imaging were 2.200 ± 0.761 and 3.162 ± 0.833 (t =-5.582,P ＜ 0.01).At 24 h post-radiotherapy,the delayed SUVmax of groups A,B and C were 2.614 ± 0.654,2.349 ± 0.869 and 5.663 ± 1.144,respectively.The differences between pre-radiotherapy and 24 h post-radiotherapy were statistically significant in all three groups (t =2.527,3.620,11.011,all P ＜0.05).One week after radiotherapy,the delayed SUVmax of groups A,B and C were 3.625 ± 1.064,3.058 ±0.850 and 7.424 ± 1.751,respectively.The differences among groups A,B and C were statistically significant (tA∶ B =2.652,tA∶C =3.799,tB∶C =4.366,all P ＜0.05).The cancer cells of group B were fewer than those of groups A and C by pathological findings,which was consistent with 18F-FDG PET/CT results.The survival times of groups A,B and C were (62.375 ±4.534),(69.000 ±4.660) and (54.125 ±5.276) d,respectively.Kaplan-Meier survival curves revealed better survival of group B as compared to groups A and C,respectively (Log-rank,x2 =7.355,16.943,both P ＜ 0.01).Conclusion 18 F-FDG PET/CT is able to evaluate the effect of irisquinone on tumor radiosensitivity enhancement.

Adolescent ; Classical Lissencephalies and Subcortical Band Heterotopias ; diagnosis ; Female ; Humans

Through the research training of undergraduate students of pharmacy utilizing medicinal chemistry research platform, not only the undergraduate teaching can be facilitated, but also the creative ability, communications skills, ability to work independently and team spirit of students can be developed,which will be helpful to cultivating high quality pharmacy talents, so as to meet the requirement of today's pharmaceutical companies and research institutes.

Aim To investigate the protective effects of Aplysin on ethanol-induced oxidative damage in rat pri-mary hepatocytes. Methods Rat primary hepatocytes were obtained via the portal vein collagenaseⅣin situ perfusion technique followed by a Percoll density gradi-ent centrifuge. MTT test was used to determine the op-timum dose of Aplysin and ethanol, and detect the cell vitality in primary hepatocytes. Supernatants of primary hepatocytes were harvested to measure AST and LDH level, and the SOD, GSH-PX activities and MDA con-tent in primary hepatocytes were observed. Flow cy-tometry was used to detect the cell apoptosis rate. DNA damage in primary hepatocytes was detected by single-cell gel electrophoresis assay. The level of mitochon-drial membrane potential in primary hepatocytes was tested by fluorogenic probe JC-1 . The CYP2 E1 activity in primary hepatocytes was detected by colorimetry. The proteins of CYP2 E1 were detected by Western blot. Results 300 mmol·L-1 dose of ethanol and 30 mg·L-1 dose of Aplysin were the optimal dosages and were used in the subsequent experiments. Hepatocyte vitality was significantly increased in Aplysin group compared to that in ethanol group, and Aplysin inhibi-ted the release of AST and LDH(P<0. 05). For Apl-ysin treatment group, the activities of hepatocyte SOD and GSH were significantly increased, and MDA was markedly lowered as compared with those in ethanol group( P <0. 05 ) . Aplysin could alleviate hepatocyte apoptosis significantly, and hepatocyte DNA damage rates of Ⅱ ~Ⅲ level and Ⅳ level were significantly lowered in Aplysin treatment group as compared with those in ethanol group, and Aplysin had evident im-provement in alcohol induced mitochondria damage of hepatocyte. Primary hepatocyte activities and protein expression of CYP2 E1 were markedly lowered in Aply-sin treatment group as compared with those in ethanol group(P<0. 05). Conclusion Aplysin has protective effects on liver oxidative damage induced by alcohol of primary cultured rat hepatocytes by blocking CYP2 E1 activation, relieving oxidative stress, and sharpening the oxidation resistance ability.

Objective To explore the Chinese medicine treatment of QUNTAI capsule in different stages of premature ovarian failure effect.Methods Sixty SD female rats were divided into 6 groups:normal group,model group,prevention group,intervention group,the treatment group,and Western medicine group.To observe the changes of behavior in rats,enzyme-linked immunosorbent assay (ELISA) was used to detect the levels of follicle stimulating hormone FSH,LH,serum estradiol E2,and anti Mueller hormone AMH.RT-PCR was used to detect ovarian tissue vascular endothelial growth factor (VEGF) and basic effect of fibroblast growth factor (bFGF) mRNA expression.Results The levels of FSH and LH in the normal group and the administration group were significantly lower compare to model group (P＜0.05),and the contents of AMH and E2 were significantly higher compare to model group (P＜0.05).The FSH and LH content in the model group was significantly higher than that of the normal group and each treatment group,E2,AMH content was significantly lower than that of the normal group and each treatment group (P＜0.05);the expression of VEGF andbFGF mRNA in each treatment group and nornial group was significantly higher than that of model group (P＜0.05),Prevention group was higher than intervention group and treat ment group (P＜0.05),there was no Gignificont difference between prevention group and Western medicine group(P＞0.05).Conclusion QUNTAI capsule can increase serum E2 and AMH levels,lower serum FSH,LH levels,improve ovarian sensitivity to sex hormone levels,repair damaged ovarian tissue,promote follicular growth and development,Thereby inhibiting premature follicle depletion.