Objective To explore clinical effects of laparoscopy applied in the female patients with infertility. Methods 468 cases of female infertility patients were diagnosed and treated by laparoscopy and 168 cases received follow-up survey. Results Among those infertility patients, the patients of normal pelvic cavity and the abnormal one were 49(10.47% ) and 419( 89.53% ). Among those patients of abnormal pelvic cavity .fallopian tube disease in 276 cases(65.87% ) was at the first place. The constituent ratio of endometriosis,ovary tumor,polycystic ovary,uterine myoma,female genital tract malformation and pelvic tuberculosis was 14. 80% ,5. 97% ,4. 30% ,3. 82% , 3. 34% and 1.91% .respectively. The patients of primary infertility and the secondary ones were 208 and 260 cases. In secondary infertility patients 69.23% (180/260) of patients were caused due to fallopian tube diseases,and the constituent ratio was higher than that in primary infertility 46.15% (96/208) (x2 = 14.05 ,P <0.05),while in primary infertility,the constituent ratio of endometriosis and normal pelvic cavity were 20.19% ,15.38% ,which were higher than that in secondary infertility respectively(x2 = 11. 78,11. 16, all P < 0.05). The pregnancy rate of 168 cases followed-up was 45.24% (76/168).47.37% ,84.21% pregnancy occurred within 6,12 months with laparoscopy. The shortest and longest time of pregnancy was 2 and 30 months, respectively. The median was 8months. Conclusion Laparoscopy playd an important role in diagnosis of infertility and improvement of pregnancy rate.

Objective To disclose the relationship of the target muscle function and different time interval after nerve grafting reconstructed C5 root resection in young rats.Methods Model of C5 resection was set up in 48 18-day-old SD rats.The rats were randomly divided into C5 resection group,immediate repairing group,3 days delayed repairing group,and 6,9,12,15,18 days delayed repairing groups.Each group experienced nerve grafting bridged the C5 nerve root defection at its time interval.At 6 weeks postoperatively,electrophysiological and histochemical experiment were performed.Results There was no statistical difference among the data of CMAP amplitude and latency and weight of target muscles and number of distal myelinated fiber of immediate repairing group and those of 3,6 days delayed repair group at 6 weeks postoperatively,but compared with C5 resection group,the dates was statistically higher.There was no statistical difference between the data of C5 resection group and that of 15,18 days delayed repairing group.Conclusion Nerve reconstruction for C5 root injury in young rats within 0-6 days (equal to 0-4 months in human beings) has a satisfactory protective effect on target muscles.It suggests that the OBPP children who have the operation indication should undergo surgical management in 4 months after their birth.

Objective To investigate the difference of the glial cell line-derived neurotrophic factor and its receptor content of proximal neurons after nerve grafting was used to reconstruct C5 root in young rats.Methods Model of C5 resection was set up in 12 18-day-old SD rats.Experimental animals were divided in to two groups, one group for C5 resection, another for nerve grafting to reconstruct the C5 defection.At 4 weeks postoperatively, the immunohistochemical staining was performed and the number of GDNF and GFRa1 immunohistochemical positive neurons were calculated respectively.Results The number of GDNF positive neurons in spinal cord and dorsal root ganglion of C5 repairing group was 786.3 ± 176.84 and 2997.0 ±357.99, and that of C5 resection group was 335.0 ± 49.50 and 1632.0 ± 305.55.On the other hand, the number of GFRa1 positive neurons in spinal cord and dorsal root ganglion of C5 repairing group was 787.5 ±178.55 and 3111.0 ± 445.72, that of the other group was 397.3 ± 41.78 and 1588.3 ± 229.00.The statistical analysis result showed GDNF and GFR immunohistochemical positive neurons in spinal cord and dorsal root ganglion of C5 repairing group was statistically more than that of C5 resection group(P < 0.01 ).Conclusion The neuronal protective effect of nerve grafting after reconstructing brachial plexus nerve injury in young rats may be attributed to the increase of GDNF and its receptor GFRa1 content of proximal neuron.

Objective To disclose the relationship of neuronal protective effect and different time interval after nerve grafting reconstructed C5 root resection in young rats.Methods Model of C5 resection was set up in 18-day-old SD rats from Jauary 2009 to December 2009.Forty-eight rats with C5 resection were randomly divided into C5 resection group,immediate repairing group,three days delayed repairing group,and 6,9,12,15,18 days delayed repairing groups.Each group experienced nerve grafting bridged the C5 nerve root defection at its time interval.At 4 weeks postoperatively,the numbers of True Blue positively labeled neurons in all groups were counted respectively.Results There was no statistical difference among the number of proxinal neuron of immediate repairing group and those of 3,6 days delayed repair group (P ＞ 0.05),but compared with C5 resection group,the number of neurons was statistically higher (P ＜ 0.05).There was no statistical difference between the number of motoneurons of immediate repairing group and that of 9 days delayed repairing group(P ＞ 0.05),but there was statistical difference between sensory neurons of this two groups(P ＜ 0.05).The neuron number of inmediate repairing group was statistically higher than those of 12,15,18 days delayed repairing group(P ＜ 0.05).Conclusion Nerve reconstruction for C5 root injury in young rats within 0-9days (equal to 0-6 months in human beings) has a satisfactory protective effect on proximal neuron.It suggests that the OBPP children who have the operation indication should undergo surgical management in 6 months after their birth.

Objective To investigate the effect of nerve grafting to enzyme histochemical changes on neurons after brachinal plexus nerve in jury in young rats. Methods Model of C5 resection was set up in 24 18-day-old SD rats. Experimental animals were divided in to two groups, one group for C5 resection, another for nerve grafting in repairing the C5 defection. At 4 weeks postoperatively, cholinesterase (CHE) and acidphosphatase (ACP) histochemical stain of neurons in C5 anterior horn and dorsal root ganglia (DRG) were detected. Results Compared with C5 resection group, bio-activity of CHE of C5 repairing group was statistically higher, and that of ACP was statistically lower. Conclusion Nerve grafting has protective effect on survival of neurons after brachial plexus nerve injury in young rats.

Objective To study the clinical significance of changes of plasma nitric oxide (NO) and tumor necrosis factor (TNF) levels in patients with congestive heart failure (CHF).Methods Plasma NO and TNF in 68 CHF cases were tested.30 healthy subjects served as normal controls.Results Plasma NO and TNF levels in CHF patients were obviously higher than those in normal subjects (P

Objective To establish a LC-MS analytical method for determination of N,N-dimethylaniline which is genotoxic impurity in quetiapine fumarate.Methods The method was achieved by an Waters ACQUITY UPLC CSHTM PhenylHexyl(2.1 mm× 100 mm,1.7 μm) utilizing a mobile phase of buffer-methanol(900∶ 100) (A)-acetonitrile(B) with gradient elution at the flow rate of 0.4 mL·min-1.The temperature of column was set at 50 ℃;The DIONEX Ultimate 3000 HPLC-AB Science 4000 QTrap Tripling Four bar LC-MS to detect N,N-Dimethylaniline (ESI source,in MRM positive mode).Results Standard curve was linear in the range of 0.4-8.0 ng(r =0.999 3);The limit of detection was 0.2 ng;The limit of quantification of N,N-dimethylaniline was 0.4 ng,respectively.The average recovery of N,N-dimethylaniline was 103.3 ％;RSD was 4.3％ (n =9),respectively.Conclusion The method is convenient and sensitive for the determination of N,N-dimethylaniline in quetiapine fumarate.

Objective: To study the influence of morphine on the expression of nestin in the ependymal epithelia, central gray and hippocampal formations in mice. Methods: Twenty health mice were evenly randomized into control group and experiment group. Mice in the control group were injected with normal saline (0.1 ml daily) and those in the experimental group were injected with morphine (0.1 ml, 1 mg daily). Thirty days later, the mice brain samples were harvested and made into paraffin sections. Immumohistochemical ABC technique was used to observe the expression of nestin under light microscope. The images were analyzed with the image analytical system. Results: In the control group, the ependymal epithelia, the central gray, the periventricular gray substances and the hippocampal formations had weak expression of the nestin, with a mean gray scale of 150.98±13.31; there were 5 kinds of nestin-positive cells: (1) the basal cells of ependymal epithelium, (2) cells distributed in the periventricular gray substance and the deep lamella of central gray, (3) cells distributed in the superficial lamella of central gray, the subiculum, the parahippocampal gyrus and the cortex in II, III layers of the entorhinal area, (4) cells frequently seen in the tectum of rnidbrain and the subiculum, and (5) cells distributed in the tectum of midbrain, the hippocampus, gyrus dentatus, parahippocampal gyrus and the cortex in V layer of the entorhinal area; the density of nestin in the subiculum and entorlimal area was (7.20 ± 1.23) mm2. In the experiment group, the ependymal epithelia, the central gray, the periventricular gray substances and the hippocampal formations had positive expression of the nestin, with the mean gray scale being 133.03 ± 22.28; the density of the above-mentioned 5 kinds of cells increased; the density of nestin in the subiculum and entorhinal area was (10.50±1.43) mm2. The mean value of gray scale and nestin-positive neurons were significantly different between the 2 groups (P<0.05). The cellular proliferation was seen in the ependymal epithelia. The second kind of cells appeared in the superficial lamella of central gray, parahippocampal gyrus and the cortex in I, II and III layers of the entorhinal area. The third kind of cells increased in the hippocampal pyrarnidal layer and the fourth kind of cells increased in the tectum of midbrain and the subiculum. Conclusion: Morphine can promote nestin expression in the ependymocyte, the central gray, the periventricular gray substance and the hippocampal formation; it can also promote the proliferation, differentiation and migration of the ependymocytes and neurocytes.

Objective: To study the influence of morphine on the expression of synaptophysin (SYN) and synapse structure in mice hippocampus, so as to provide pathological evidence for studying the development and treatment of chronic morphine intoxication, addiction and abstinence symptoms of morphine. Methods: Twenty mice were evenly randomized into control group and experiment group. Mice in control group were injected with normal saline (0.1 ml daily) and those in experimental group were injected with morphine (0.1 ml, 1 mg daily). Thirty days later the mice in 2 groups were killed and their brain tissues were harvested and made into slices, stained with immunohistochemical techniques (SP) and photographed under the light microscope. The images were analyzed with the image analytical system and the data were statistically analyzed. Results: In the control group, positive staining of SYN was found in the entorhinal area, subiculum, stratum plextiforme, polymorphic layer of gyrus dentatus, stratum oriens, and stratum radiatum of hippocampus; weak positive staining of SYN was noticed in the stratum lacunosum-moleculare of hippocampus; positive staining of SYN was also found the membrane of pyramidal cells and granule cells, with the mean gray scale value of the hippocampal structure being 132.84 ± 8.67. Positively stained neurons was also found in the entorhinal area and the subiculum, with a intensity of (7.80 ± 1.03)/mm2. In the experiment group, the subiculum and polymorphic layers of gyrus dentatus were positively stained for SYN; the entorhinal area, stratum oriens, stratum radiatum and stratum lacunosum-moleculare of hippocampus were strongly positive of SYN; the membrane of pyramidal cells and granule cells were also strongly positive of SYN, with the mean gray scale value of the hippocampal structure being 116.27 ± 5.70. Strongly stained neurons were also found in the entorhinal area and the subiculum, with the intensity being (11.90 ± 1.45)/mm2. The number of SYN positive neurons and the intensity of SYN in the experimental group were higher than those in the control group (P<0.01). The hippocampal strucure looked like ground glass, with slight staining and many pyknotic nuclei and bubbles. Conclusion: Morphine can promote the SYN expression and apoptosis of neurons in the hippocampus, and therefore affect the structure and the function of the synapses in it.

Antineoplastic Agents ; administration & dosage ; adverse effects ; Asparaginase ; administration & dosage ; adverse effects ; Child ; Child, Preschool ; Combined Modality Therapy ; Dose-Response Relationship, Drug ; Drug Hypersensitivity ; prevention & control ; Female ; Humans ; Hyperglycemia ; chemically induced ; prevention & control ; Male ; Precursor Cell Lymphoblastic Leukemia-Lymphoma ; drug therapy ; Severity of Illness Index ; Time Factors ; Treatment Outcome