Objective:The influences of detection of the preoperative level of serum CA19-9 were analyzed on the prognosis of gastric cancer patients. Methods:A total of 513 gastric patients with preoperative CA19-9 detection were enrolled and underwent radical gastrectomy in Tianjin Medical University Cancer Institute and Hospital from January 2003 to October 2008. Clinico-pathological variables associated with the CA19-9 level were analyzed, and the prognostic value of CA19-9 was evaluated. Results:Eighty-six (16.8%) patients manifested an increased CA19-9 level, which was associated with ageing, Borrmann typesⅢandⅣ, undifferentiated type, and advanced T stage. The five-year survival rates were 45.7%and 25.6%for patients with normal (<39 U/mL) and significantly high CA 19-9 levels (≥39 U/mL), respectively. Differences in survival rates between the patient groups were statistically significant (P<0.001). Tumor-node-metastasis (TNM)-stratified analysis revealed a difference in overall survival for patients with stageⅢtumors. The significantly increased CA19-9 level was an independent prognostic factor for gastric cancer patients after radical surgery based on multivariate analysis. Conclusion:Detection of preoperative level of serum CA19-9 could provide important information for prognostic evaluation of gastric cancer patients. CA19-9 was a potential independent prognostic factor for gastric cancer patients after surgery.

Objective To investigate the inhibitory effects of hepatitis B virus (HBV) S gene-specific antisense locked nucleic acid (LNA) on HBV replication and expression in HepG_22.2.15 cells,and to screen the effective short sequence of LNA.Methods Four different lengths of short sequence of antisense locked nucleic acid which were complementary to the initiator of HBV S gene were designed,synthesized and transfected by cationic liposomes into HepG_22.2.15 cells.The HBsAg and HBV DNA of supematant was tested by enzyme linked immunoadsorbent assay(ELISA) and real-time fluorescent quantitative PCR(FQ-PCR) at 24,48 and 72 hours after treatment.LNA's cyto-toxicity on cell was evaluated by MTT method.Results Four different lengths of short sequence of LNA inhibi-ted the expression of HBsAg and the replication of HBV DNA with the inhibition rates of 46.58%,54.38%,72.43% ,69.92% and 27.09% ,28.77% ,34.71% ,32.68% respectively after 72 hours.There's no obvious tox-icity on cell.Conclusion Antisense LNA that targeting at HBV S gene has strong inhibition on HBV in vitro,and the optimal length of LNA sequence might be in the range of 15 base to 25 base.It has a therapeutic potential in the treatment of patients infected with HBV.

Objective To investigate the effects of Buyang Huanwu Decoction(BHD) and two kinds of fractions extracted from BHD on expression of caspase-1,caspase-3,and caspase-8 mRNA after cerebral ischemia-reperfusion in rats.Methods The model of cerebral ischemia-reperfusion was induced by the middle cerebral artery occlusion(MCAO) in rats,via string ligation of arteria carotis interna.The expression of caspase-1,caspase-3,and caspase-8 mRNA in cerebral ischemic tissues was assessed by reverse transcription-polymerase chain reaction(RT-PCR).Results The expression of caspase-1,caspase-3,and caspase8 mRNA in cerebral tissues was significantly increased after 22 h reperfusion following 2 h cerebral ischemia in model group(P

Objective To compare the efficiency and safety of clopidogrel of different loading-dose in acute coronary syndromes(ACS) patients with percutaneous coronary intervention(PCI) therapy, and, give an assessment of security about higher loading dose of clopidogrel. Methods 120 patients from January 2008 to January 2009 in our hospital were included.They were randomly divided into 3 groups(na=nb=nc=40)and were given 600 mg,450 mg and 300 mg clopidogrel on the 6 hour before PCI. There was no significant difference between the three groups' basic clinical information(p>0.05),such as age, sex and so on. Four points were selected to observe: the platelet aggregation induced by ADP with 5umol/L before and two hours、four hours, six hours after taking medicine; Primary endpoint events, which include shock during hospitalization, death, target revascularization, myocardial infarction, recurrent angina, stroke in 30 days,and Postoperative Bleeding events and adverse Reactions after Surgery in 30 days. Results compared to the standard dose of 300 mg clopidogrel, 600 mg doses clopidogrel can give a greater degree of inhibition on platelet activating than 450 mg doses in the pre-6 h after administration. Increase Clopidogrel loading dose can reduce the occurrence of major cardiovascular events rate. Bleeding events and the occurrence of adverse events was no significant difference among three groups. Conclusions compared to the standard dose of 300 mg clopidogrel, a higher loading dose of clopidogrel can produce much more faster, greater platelet inhibition, and similar security.

OBJECTIVE: To investigate the effects of Panax notoginseng saponins (PNS) on mRNA expressions of interleukin-1 beta (IL-1 beta), interleukin-1 receptor type I (IL-1RI), interleukin-1 receptor antagonist (IL-1ra), intercellular adhesion molecule-1 (ICAM-1), cysteinyl-aspartate specific protease-1 (caspase-1), caspase-3 and caspase-8 after cerebral ischemia-reperfusion in rats. METHODS: Focal cerebral ischemia reperfusion in rats was induced by the method of nylon monofilament via the internal carotid artery. PNS was administered intraperitoneally respectively five minutes before cerebral ischemia and twelve hours after cerebral ischemia. After cerebral ischemia for two hours followed by reperfusion for twenty two hours, the mRNA expressions of IL-1 beta, IL-1RI, IL-1ra, ICAM-1, caspase-1, caspase-3 and caspase-8 in brain tissue were determined by reverse transcription polymerase chain reaction assay. RESULTS: After cerebral ischemia for two hours followed by reperfusion for twenty two hours, the mRNA expression levels of IL-1 beta, IL-1RI, IL-1ra, ICAM-1, caspase-1, caspase-3 and caspase-8 in brain tissue in the untreated group were obviously elevated as compared to those in the sham-operation group (P<0.05 or P<0.01). The mRNA expression levels of IL-1 beta, IL-1RI, IL-1ra in brain tissue in the PNS group were lower than those in the untreated group, but higher than those in the sham-operation group, and without statistical differences as compared to those in the sham-operation group and in the untreated group (P>0.05). The mRNA expression level of caspase-3 in brain tissue in the PNS group was significantly lower than that in the untreated group (P<0.05), but PNS had no effect on the mRNA expression levels of ICAM-1, caspase-1 and caspase-8 in brain tissue. CONCLUSION: PNS can inhibit the mRNA expression of caspase-3, slightly inhibit the mRNA expressions of IL-1 beta and its correlative inflammatory factors in brain tissue. The protective effects of PNS on cerebral injury induced by ischemia-reperfusion may be related to inhibiting the mRNA expressions of caspase-3, IL-1 beta and its correlative inflammatory factors in brain tissue.

Objective To evaluate the expression of STAT3, VEGF and Survivin in human gastric carcinoma and its clinicopathological significance. Methods The expression of STAT3, VEGF, survivin was determined by immunohistochemical staining of specimens from 53 cases undergoing radical gastrectomy and 53 cases of normal gastric mucous membranae. We evaluated the relationship between expression of these proteins and various clinicopothological factors. Results The expression rate of STAT3, VEGF and survivin in 53 gastric carcinoma tissues was 58%, 62% and 74%, respectively, which was significantly higher than those in the normal group(P <0. 01). STAT3 expression correlated with VEGF(r =0. 608 ,P <0. 01) ,survivin(r = 0. 451, P = 0. 001). Positive STAT3, VEGF staining was significantly associated with tumor size, Lauren's classification,lymph node metastasis and clinical staging(P < 0. 05). Survivin staining was significantly associated with Lauren's classification, lymph node metastasis and clinical staging(P <0. 05). Multivariate analysis revealed STAT3 expression and lymph node metastasis were independently prognostic factors of poor survival. Conclusion VEGF, survivin possibly regulated by STAT3 leads to tumor angiogenesis and anti-apoptosis. The expression of STAT3 is an independent prognostic factors in gastric carcinoma.

Objective To confirm whether miR-216a suppresses cell proliferation and induces cell apoptosis by targeting PKCα, thus to reveal molecular mechanism that miR-216a functions as a tumor suppressor in gastric cancer .Methods PKCα3′untranslat-ed region(UTR)-luciferase vector was constructed and dual-luciferase reporter gene assay was employed to examine the effect of miR-216a on luciferase activity .MGC-803 cells were transfected with miR-216a mimics ,and next Western blotting was performed to detect the expression of PKCαprotein .The effects of PKCαdownregulation on cell proliferation and apoptosis were observed after PKCαsiRNA were transfected into MGC-803 cells .MGC-803 cell proliferation assays were performed when cotransfected with miR-216a mimics .Results The result demonstrated miR-216a could bind to the 3′UTR of PKCαand inhibited the luciferase activi-ty ,cut the 41% .PKCαprotein expressions were significantly down-regulated when miR-216a was overexpressed in MGC-803 .siR-NA-mediated downregulation of PKCα could suppress the potentials of cell proliferation and induce apoptosis .Conclusion miR-216a suppresses cell proliferation and induces apoptosis by targeting PKCαmRNA 3′UTR in gastric cancer .

Objective To evaluate the efficacy and safety of itraconazole injection in treatment of invasive fungal infection in the patients with multiple organ dysfunction syndrome.Methods Invasive fungal infection in 15 patients with multiple organ dysfunction syndrome was treated with 200 mg itraconazole injection,twice a day on the first two days,then once a day,for 8 to 14 days.During the treatment,the symptoms and signs,the results of chemical detection were recorded.Everyday fungus,sputum,stool smear or culture were performed.Results The recovery rate,effective rate,and fungal clearance in this trial with itraconazole injection were 80%(12/15),86.7%(13/15),and 86.7%(13/15) respectively.The side effect was not found.Conclusion Itraconazole injection is an effective and safe drug against invasive fungal infection in patients with multiple organ dysfunction syndrome.

0.05).Ticlopidine could inhibit thrombosis induced by ferric chloride,but it did not influence TXB_2 and 6-keto-PGF_1? content and activities of AT-Ⅲ and PC in the plasma.Ticlopidine could enhance activity of t-PA in the plasma.It may be related to the decrease of t-PA consumption by inhibiting thrombosis.Antithrombosis action of ticlopidine may not be concerned to inhibiting production of TXB_2 of platelet.LMWH could inhibit thrombosis also,but it did not influence TXB_2 and 6-ketoPGF_(1?) content in the plasma.LMWH could enhance activities of t-PA and t-PA/PAI-1 ratio,which may be related to the promoting release of t-PA in vascular endothelial cells.LMWH could reduce activity of AT-Ⅲ,which may be concerned with combination of LMWH and AT-Ⅲ result in AT-Ⅲ consumption.Conclusion Ferric chloride may induce occlusive thrombosis in rats.Thrombosis may be associated with activation of platelet and blood coagulation system,lower of anticoagulative protein and fibrinolytic activity.

Aim To investigate the growth-inhibition and the mechanism of apoptosis induced by sophoridine(SRI) on SW620 cells.Method MTT assay was used to detect the half-inhibition concentration(IC50),and fluorescence microscopy,electron microscopy,DNA fragmentation analysis,flow cytometry(FCM) were used to demonstrate the presence of apoptosis.Results SRI inhibited the growth of SW620 cells significantly in a dose-and time-dependent manner,and morphological characteristics of apoptosis were observed with condensation of nucleus,bubble of cytoplasm,fragment of nucleus.A DNA ladder pattern of internucleosomalfragmentation was observed.Compared with that of control group,the percentage of the G0/G1 phase and the Sphase cells increased after treated by SRI.SW620 cells were induced to undergo apoptosis and underwent G0/G1 arrest with exposure to SRI shown by FCM.Conclusions Sophoridine could induce the inhibition of cell growth by means of apoptosis in a dose-and time-dependent manner,and the blocking of G0/G1 phase of cells was involved in the mechanism of apoptosis.