Intrauterine growth retardation( IUGR) is a common complication during pregnancy. Epide-miological and clinical studies have shown that IUGR has a long-term impact on postnatal lipid metabolism. The effect of IUGR on postnatal lipid metabolism is accomplished by regulating signal pathways of fatty acid and cholesterol metabolism,which may be mediated by epigenetic modifications. Further study of these epigenetic mechanisms could lead to a novel way for prevention and treatment of adulthood diseases, including dyslipi-demia,non-alcoholic fatty liver disease and metabolic syndrome.

Adult ; Aged ; Aged, 80 and over ; Antineoplastic Agents ; adverse effects ; therapeutic use ; Brain Neoplasms ; secondary ; Carcinoma, Non-Small-Cell Lung ; drug therapy ; secondary ; Exanthema ; chemically induced ; Female ; Humans ; Liver Neoplasms ; secondary ; Lung Neoplasms ; drug therapy ; pathology ; Male ; Middle Aged ; Neoplasm Staging ; Protein Kinase Inhibitors ; adverse effects ; therapeutic use ; Quinazolines ; adverse effects ; therapeutic use ; Remission Induction ; Survival Rate ; Vomiting ; chemically induced

Objective The diagnosis of low frequency sensorineural hearing loss is examined for clinical applications.Methods Pure tone threshold(PTT),acoustic impedance,auditory brainstem response(ABR),distortion product otoacoustic emissions(DPOAE),electrocochleogram(ECochG)and imaging examinations of CT or MRI were performed on 56 cases with low frequency sensorineural hearing loss.Results Among 56 cases of low frequency sensorineural hearing loss,38 cases with sudden low frequency sensorineural hearing loss,9 with Meniere's disease, 6 as auditory neuropathy,1 case of acoustic neuroma,1 case of multiple sclerosis and 1 case with arachnoid cyst of cerebellar hemisphere surface.Conclusion Low frequency sensorineural hearing losses occurred in varied diseases.A comprehensive approach should be taken to diagnose this hearing loss and if necessary,CT or MRI can be utilized to confirm the standard audiologic evaluations.

Objective The diagnosis of low frequency sensorineural hearing loss is examined for clinical applications.Methods Pure tone threshold (PTT),acoustic impedance,auditory brainstem response(ABR),distortion product otoacoustic emissions(DPOAE),electrocochleogram(ECochG) and imaging examinations of CT or MRI were performed on 56 cases with low frequency sensorineural hearing loss.Results Among 56 cases of low frequency sensorineural hearing loss,38 cases with sudden low frequency sensorineural hearing loss,9 with Meniere's disease,6 as auditory neuropathy,1 case of acoustic neuroma,1 case of multiple sclerosis and 1 case with arachnoid cyst of cerebellar hemisphere surface.Conclusion Low frequency sensorineural hearing losses occurred in varied diseases.A comprehensive approach should be taken to diagnose this hearing loss and if necessary,CT or MRI can be utilized to confirm the standard audiologic evaluations.

The nature and functions of astrocytes (AS)， the pathological changes and roles of AS after spinal cord injury， the experimental methods of inhibiting AS proliferation and glial scar formation， and so on, were reviewed. AS beneficially affects repairing injured spinal cord at different periods of differentiation. However， adult AS secreting the factors formed the chemical glial barrier which severely affects nerve regeneration and hinders axon extension. Because of the static， activated and proliferative AS tending to coexist after spinal cord injury， and complex factors of barrier formation， the current approach taken by a single method is difficult to effectively control the AS proliferation and glial scar formation.

Objective To explore the methods of culture and identification of human placental microvascular endothelial cells(HPMVEC) in vitro,aiming to construct the polar model of human placental barrier.Methods By proteolytic enzymes,the tissular and cellular suspensions were collected from the chorionic villi of pregnant and centrifugated over a preformed discontinuous Percoll gradient.The highly purified human placental microvascular endothelial cells were obtained and cultured in HG-DMEM with fetal calf serum and identified by immunocytochemistry(SP methods) and transmission electron microscope.Results The highly purified human microvascular endothelial cells were cultured in vitro and passaged for three to five generations.Factor Ⅷ-related antigen and CD34 were both positive by immunocytochemistry.Weibel-Palade body(W-P body) was observed by transmission electron microscope.Conclusion Human placental microvascular endothelial cells can be isolated and cultured successfully in vitro by our method.

Objective To investigate the efficiency,decision of intra-operative puncture route,treatment of perioperative complications and discuss the other relative problems of the treament for lumbar disc herniation with percutaneous transforaminal endoscopic discectomy.Methods To excise the nucleus pulpesus under percutaneous transforaminal endoscopic discectomy,use the Macnab standard,visual analogue scale and infrared thermal imaging to estimate the efficiency.Results Among followed-up of 208 patients,182 patients were excellent and good outcome,23 patients favorable,2 patients fair,0 patient poor.The leg and back VAS was a significant improvement 1 week post-operation compared with pre-operation (P ＜ 0.05),but no statistical difference among 3 months,6 months,12 months and 1 week post-operation (P ＞ 0.05).The infrared thermal imaging point out that the legs skin temperature of D-value was a significant improvement post-operation compared with pre-operation (P ＜ 0.05).Conclusions The method excised the nucleus pulpesus,and provided the spine maximum protection about the stability and flexibility.Intra-operative puncture route of individuation design can reduce the complications of intervertebral foramen perioperative,and the key to improve the effectiveness.

Background and purpose:Pancreatic cancer is one of the most deadly human malignant neoplasms. Resistance to chemotherapeutic drugs is a major reason responsible for poor prognosis in the treatment of pancreatic cancer patients. MicroRNA (miRNA, miR) is a family of small non-coding RNA molecules, dysregulated miRNA is associated with various tumor biological function. miR-33a has been widely reported as a metabolism-related miRNA, while its relationship with drug resistance has little understand. This study was focused on the effect of miR-33a on gemcitabine chemoresistance in pancreatic cancer to bring the novel theoretical basis to chemotherapy for pancreatic cancer.Methods:In situ hybridization and Real-time PCR were used to analyze the miR-33a expressions in pancreatic cancer tissue sample and cell lines, respectively. Cell counting kit 8 (CCK-8) assay was used to calculate the IC50 value of different pancreatic cancer cells.Results:miR-33a was down-regulated in pancreatic cancer tissue and cell lines compared with para-cancerous tissues and normal HEK293T cells. Moreover, miR-33a over expression not only could enhance the chemosensitivity to gemcitabine in pancreatic cancer cells, but also rescue the gemcitabine resistance in pancreatic cancer cells.Conclusion:Down regulation of miR-33a in pancreatic cancer decreases the chemosensitivity to gemcitabine, resulting in development of acquired gemcitabine chemoresistance. It provides the theoretical basis to develop a new molecular targeted drug to combine with chemotherapy for pancreatic cancer.

Objective To study core needle biopsy in diagnosis of palpable breast cancer.Methods Retrospectively analyze core needle biopsy in 200 cases of palpable breast cancer.We recorded the clinical and pathology data and used crosstable test to analyze the effect of maximal and minimal tumor size.and TNM stage on the results. Results The ratio of success was 97%. No significant difference was found among the groups of different maximal tumor size, minimal tumor size and TNM stage (P ＞ 0.05 ). Conclusion ( 1 )For the diagnosis of the suspicious malignant palpable mass, core needle biopsy is effective, safe, microtraumatic and suitable to be used in different-class hospitals ; (2) The success of core needle biopsy for palpable breast cancer is correlated with dexterity of operator; (3)Biopsy channel should be in the range of operation and avoid of affecting sentinel lymphatic channels.

Objective To investigate the action of ulinastatin in protection of hepatic-renal function in patients with acute myocardial infarction (AMI) receiving emergency percutaneous coronary intervention (PCI). Methods 104 patients with acute myocardial infarction were randomly selected and divided into a study group (n = 55) and a control group (n = 49). The study group received intravenous ulinastatin of 300,000 units one hour before PCI, and 300,000 units daily for 3 days after the procedure; while the control group received the same amount of normal saline instead of ulinastatin before and after PCI. Levels of AST, ALT, and CRE were compared between the two groups and CCR was counted before and 72 hours after the procedure. Result Serum AST level was increased and CCR was decreased after PCI; and the change in the study group was lower than that in the control group (P < 0.05). ALT level was declined in the study group but was elevated in the control group after the procedure, with a significant difference between the two groups (P < 0.05). Conclusions Ulinastatin can protect hepatic-renal function in patients undergoing emergency PCI, lowering contrast-induced nephropathy and damage of liver and kidneys.