Objective To investigate the genetic patterns of iris pigmentat mutation in the white hair black eye ( WHBE) rabbits.Methods To construct six two-generation families between WHBE rabbit and Japanese white rabbit, and the quantity of individuals had different eye colours in F1 and F2 generations were recorded and analyzed.Results χ2 test showed there was no significant difference between observed values and expected values in the mode of autosomal domi-nate inheritance ( P>0.05) , while there were significant differences between observed values and expected values in the autosomal recessive inheritance and sex-linked genetic pattern(P<0.05).Conclusions Iris pigmentat mutation in WHBE rabbit has a monogenic character due to autosomal dominant mutation.

China ; Genetic Predisposition to Disease ; Humans ; Hypertension ; genetics ; Retrospective Studies

Female ; Humans ; Maternal Health Services ; Maternal Mortality ; Pregnancy

China ; Humans ; Occupational Diseases ; prevention & control

Aldosterone ; blood ; Humans ; Hyperaldosteronism ; classification ; diagnosis ; Hypertension ; complications ; Mass Screening ; Renin ; blood ; Renin-Angiotensin System

G protein-coupled receptors(GPCRs)mediate cell signaling transduction of most hormones and neurological transmitters and behave as the key targets for drug research and development.Recently,the evidence of allosteric modulation of GPCRs has been revealed.Allosteric modulators have the ability to selectively tune responses only in tissues in which the endogenous agonist exerts its physiological effects,and have the potential for greater receptor subtype selectivity.The GPCRs allosteric modulators have been found and some of them have been in clinical use.Under the strategy of allosterism on structure activity relationship and target directed screening,more and more GPCRs allosteric modulators will be developed in the future.

Objective To study effect of traditional Chinese medicine chrysanthemum decoction on RSV infection after A549 cells chemotactic factor.Methods Subjects from The Second Hospital Affilated To Suzhou University in recent days, 64 cases of patients diagnosed with RSV effected pneumonia by clinical examination, RSV infection A549 cell model was established and the infected cells were cultured, chrysanthemums with TCM decoction and conventional antiviral observed its effect on RSV infection cell survival, compared two groups of cell SAP was determined by ELISA kit and the expression of RANTEs and chemokines MCP-1.Results Chrysanthemum, according to the results of in vitro cell culture studies traditional Chinese medicine decoction had the effect of directly inhibit the growth of virus replication, and there were some positive effects RSV infected A549 cells, MCP-1 and RANTEs expression or release significantly increased ( P<0.05 ) .Chrysanthemum Chinese medicine decoction and antiviral drugs after the intervention, the expression of MCP-1 and RANTES reduced significantly (P<0.05), but overall volume was still significantly higher than the normal. Conclusion Chrysanthemum Chinese medicine decoction can inhibit RSV infected A549 cells release chemokine RANTEs and MCP-1 to some extent, and then reduce the airway inflammation reaction of children.

Mesenchymal stem cell (MSC) resides in a relatively hypoxia niche (volume fraction 1％～7％)compared to normoxia (20％).MSC cultured in hypoxia condition shows different biological features from those in normoxia condition.Hypoxia inducible factor-1 α (HIF-1 α),as one of main transcription factors in hypoxia,participates in the regulation of cell proliferation,differentiation,apoptosis,glycolysis and angiogenesis.Recent researches show that HIF-lα also plays an important role in regulating the survival,differentiation and migration of MSC.It turns out that hypoxia can improve survival and migration of MSC,enhance chondrogenesis but inhibit adipogenesis through HIF-lα and its downstream genes.However,whether hypoxia promotes or impedes osteogenesis remains controversial.Such differences can be caused by variances of tissue origination,oxygen concentration and culture condition.The comparison of different researches should be based on the same experiment condition.This review focuses on the influence of hypoxia on proliferation,apoptosis,differentiation and migration of MSC.

The energy metabolism of septic patients is serious disorder.The major manifestation is protein degradation,the enhancement of glyconeogenesis and the adipose lipid mobilization.This may be link to abnormal ways of inflammatory media,the hormone secretion and ubiquitin-proteasome caused by sepsis.The discussion on high metabolic mechanism of sepsis,has great significance for guiding clinical practice,promoting targeted interventions and treatment measures and improving the prognosis of patients.This article summarized the progress on hyper metabolism in sepsis.

ObjectiveTo develop a method of location of prenatal female mouse reproductive tract based on paraffin block serial sections of female fetuses, and get high quality paraffin sections of fetuses containing female reproductive tract (FRT).And to investigate the role of Wnt5a during the development of FRT of prenatal mice. Methods The sex of fetuses at gravidity 15.5 days( G15.5d), G17.5d, G19.5d) and G21.5d was identified by polymerase chain reaction(PCR),and the female fetuses were embedded in paraffin block, the specimen was sectioned serially . One of every four sections was stained by hematoxylin-eosin(HE), and the next one being used later.The images of specimen were taken with digital camera and the serial sections were obtained. The paramesonephric duct /uterus were located and recognized. Immunohistochemistry was used to determine the location and intensity of Wnt5a staining in the middle of the paramesonephric duct / uterus. Results The morphology of the paramesonephric duct /uterus was recognized first and the sections of fetuses with paramesonephric ducts were obtained. The intensity of Wnt5a immunohistochemical staining was increased from G15.5d to G21.5d in mesenchymal cells(P<0.01).Conclusion Wnt5a plays a marked role in early period of female mouse reproductive tract, and is possible to be a key factor to induce uterus differentiation and development.