Epidermal growth factor receptor(EGFR) family plays an important role in the development of gastric cancer. EGFR-targeted therapeutic agents include extracellular monoclonal antibodies and intracellular tyrosine kinase inhibitors. Many new agents such as trastuzumab, cetuximab, panitumumab and lapatinib, have been tested in randomized phase Ⅲ clinical trials. Results from ToGA trial may shed some light on the treatment of advanced gastric cancer.

Objective To study of vascular false as the clinical effect and safety of acromastitis were treated by com -pound anisodine treatment , to provide reference for the treatment of vascular pseudo optic .Methods From June 2013 to June 2015, 132 cases of patients with vascular pseudo optic papilla in our hospital were selected as the observation objects , and were divided into observation group and control group each 66 cases according to the treatment method .The control group was treated with routine drug therapy , and the observation group in the control group on the basis of daily injections of Com -pound Anisodine Hydrobromide Injection .Two groups before and after the treatment of vision changes , and the treatment of safety and efficacy were compared .Results The curative effect:The two groups of patients after treatment were improved , but the observation group was significantly higher than the control group after treatment ;The cure rate and total effective rate of the observation group were 39.51%and 88.89%respectively, which was significantly higher than the control group of 24. 36%and 73.08%, and had better curative effect in observation group .Security aspect:The total incidence of adverse reac-tions in the observation group and the treatment group was 10.61%, significantly less than 24.24% of the control group, higher safety .Conclusion In the treatment of vascular pseudoaneurysms as papillitis drugs , the curative effect of compound anisodine is much better with better security , which can significantly improve the visual acuity of the patients .

Objective:To study the effect of exogenous hyaluronidase on proliferation of human breast cancer cells in vitro.Methods:The proliferation of human breast cancer cell line ZR-75-30 were detected by MTT-assay and flow cytometry.Results:The absorbency value(A) of ZR-75-30 in study group treated with Hyase (0.674?0.221) was significantly higher than that in control group(0.563?0.046).The percentage of phase S cells in study group(18.36?0.65) was higher than that in control group(2.19?0.10);the percentage of phase G0/G1 cells in study group(44.49?4.33) was lower than that in control group(62.14?26.97).The absorbency value(A) of MDA-MB-435 in study group (0.674?0.221) was significantly higher than that in control group(0.563?0.046).The absorbency valve(A) of MDA-MB-231 in study group (0.674?0.221) was significantly higher than that in control group(0.563?0.046).Conclusion:Exogenous hyaluronidase may promote the proliferation of breast cancer cell lines in vitro.

Tumor progression is often associated with immune suppression or the ability of the tumors to avoid immune surveillance.Immunotherapy improves the ability of the immune system to recognize and clear tumor cells with a little influence on the normal tissues.Immunotherapy is a hot spot in the research of advanced esophageal cancer.Innnunotherapy of esophageal cancer includes immune checkpoint inhibitors,adoptive cellular immunotherapy,tumor vaccines and antibody therapy.At present,a large number of clinical trials are underway to evaluate the role of immunotherapy in esophageal cancer.Checkpoint inhibitors represented by Pembrolizumab and Nivolumab,has achieved initial success in the treatment of advanced esophageal cancer to improve the prognosis and life quality of esophageal cancer patients.In the future,further studies are needed to have a research on the effects of tumor heterogeneity,prediction of therapeutic targets,and immune tolerance.

Animals ; Anti-Inflammatory Agents ; pharmacology ; Cyclooxygenase 2 ; metabolism ; Inflammation ; metabolism ; Insulin ; pharmacology ; Nitric Oxide Synthase Type II ; metabolism ; RNA, Messenger ; genetics ; Rats

Silk fiber,as a kind of natural polymer,has been used as surgical sutures in clinic for years.Silk fiber is composed of a filament core protein,termed fibroin and a glue-like coating called sericin proteins.Due to its unique mechanical properties,good biocompatibility and slow degradability,silk fiber has been put in variety of applications as a new kind of biomedical scaffolds in recent years.With the development of the processing technique,silk fiber can be processed through the new versatile processing method to form various forms of scaffolds and its surface could be modified for different purposes.Further more,it could be tailored through genetic recombination to form the silk fibroin-like polymers which has a promising potential in medical applications.

Objective To detect the level of plasma superoxide dismutase(SOD) in patients with lung cancer and effect of chemotherapy(CGM + CBP) on it,and to investigate the role of SOD in the development .of lung cancer. Methods Xanthine oxidation enzyme was availabe to detect the level of plasma SOD in 30 cases of normal control subjects and 29 cases of patients with lung cancer before and after first, second, third cycle of chemotherapy. Resuits The levels of plasma SOD in lung cancer after chemotherapy were lower than those in the control group(P＜0.01). The level of plasma SOD in lung cancer after first and second chemo-cycle was higher than that before, but lower than that of control group(P＜0.01), the differences between after third chemo-cycle and control group were not significant(P＞0.05 ). The differences in squamoun cell carcinoma and adenocarcinoma before chemothrapy and after were not significant (P＞0.05 ). The differences in squamoun cell carcinoma and adenocarcinoma and adenosquamoun carcinoma before chemotherapy and after were highly significant (P＜0.01 ). The differences in each chemo-cycle were highly significant (P＜0.01). Conclusion SOD played an important role in growth, regulation and treatment of lung cancer,and it might be a clinical index in lung cancer.

Objective To assess overall diagnostic value of SELDI-TOF-MS in early breast cancer. Methods To search and identify the relevent articals in English and Chinese from Jan. 1999 to Dec. 2007 on Medline,WeiPu full-text data base and Wan-Fang clara base, and the literatures have been integrated to review the quality rating, and the heterology examination and the meta analysis were conducted. While the summary receiver operating characteristic curve (SROC), pooled sensitivity, specificity and odds rate were calculated by SPSS13.0 software. Results 22 pertinent literatures can be indexed as a whole, of which 8 articles in-cluding 739 samples entered this Meta analysis. The pooled sensitivity, specificity and odds rate were 91.4% ,99.5% and 30.88 re-spectively. The area under the SROC curve was 0. 822 5. Conclusion SELDI-TOF-MS is valuable in diagnosis of ovarian cancer.

Hemopoietic stem cells are mostly obtained from bone marrow,umbilical cord blood and peripheral blood.Physical status of final-stage liver disease patients is poor,and these patients hardly endures large wound.Thus,it is a potential method to harvest hepatocytes following transdifferentiation using peripheral blood stem cell transplantation.It is necessary to mobilize peripheral blood when enough stem cells are needed during peripheral blood stem call transplantation.Stem cell mobilization agent contains 3 types at present:chemotherapeutic drugs,polyanion preparation such as dextran sulphate,various recombinant human hematopoietic cell stimulating factors.For patients with severe liver disease,it is safe to use various recombinant human hematopoietic cell stimulating factors.Density gradient centrifugation is widely used to isolate hematopoietic stem cells.This method can obtain leukocytic cream that is rich of mononuclear cells,and primarily enrich CD34~+ cells.With the discovery of CD34~+ different antigenic determinant monoclonal antibody,immunological technique of exactly,abundantly,rapidly,positively sorting CD34~+ cells is developed quickly,such as solid phase sorting method and flow cytometry sorting.Utilizing the high reproductive activity and multi-directional differentiation,hematopoietic stem cells are amplified in vitro and induced to differentiate into abundantly hemopoietic progenitor cells in a short period,and into directional amplification of various cells such as hepatocytas for transplantation.Presently,bone marrow hematopeietic stem cells can successfully differentiate into hepatocytas.To judge whether differentiated cells were from infused stem cells in transplanted organs,the infused cells were commonly labeled before transplantation.Labeling method includes fluorescent labeling,specific staining,genetic mark and chromosome identification.Subsequently,this was identified using immunological,molecular biological,and fluorescence chemical techniques.Presently,hematopoietic stem cell transplantation in treatment of liver disease is mostly basic experiment,but has not been applied in clinic.

Objective To investigate the roles of Thl7 cells in the immunological pathogenesis of vitiligo. Methods Forty patients with progressive or stable vitiligo and 20 normal human controls were included in this study. Enzyme-linked immunosorbent assay (ELISA) was performed to measure the level of serum IL-17, and SYBR Green I real-time RT-PCR to detect the mRNA expression of RORγt in peripheral blood mononuclear cells (PBMCs) from these patients. The correlation of the above parameters with disease activity was assessed. Results The levels of serum IL-17 and ROR-yt mRNA in PBMCs were significantly higher in patients with progressive and stable vitiligo than in normal human controls (all P< 0.01). Conclusion Thl7 cells may be closely associated with the pathogenesis of vitiligo.