AIM　The purpose is to investigate the Anti-inflammatory mechanism of Biota orientalis. METHODS　Using rat leukocytes and rabbit platelets as experimental material the biosynthesis of leukotriene, 12-hydroxyheptadecatrienoic acid(12-HHT) and 12-hydroxyeicosatetraenoic acid(12-HETE) in the cells was determined with HPLC. RESULTS　Biota orientalis was shown to inhibit the biosynthesis of leukotriene B4(LTB4) and 5-hydroxyeicosatetraenoic acid(5-HETE) with IC50 of 0.40 and 0.41　mg/ml crude herb, respectively. It was also displayed to inhibit biosynthesis of 12-HHT in the platelets. CONCLUSION　Biota orientalis leaves contain anti-inflammatory components and the anti-inflammatory mechanism was related with inhibiting metabolism of arachidonic acid.

The experiment was carried out to inquire about the possibility of strain selection and improvement in Lentinus edodes through protoplast formation and mutagenesis by UV. Mycelial growth rate, yield, and period of fructification of 105 selected regenerants from UV-treated protoplasts have been measured and compared with their parents. Several selected regenerants achieved some better qualities such as higher yield and earlier fruiting. Several repeated cultivations of these regenerants reconfirmed their new capacities. The results indicated that protoplast formation and mutagenesis are the potential methods for strain selection and improvement in edible fungi.

Objective To quantify the mRNA of 5-lipoxygenase (5-LO) in the rat peritoneal macrophages cultured in vitro by capillary electrophoresis (CE). Methods The rat peritoneal macrophages were isolated and cultured for indicated time. The mRNA of 5-LO was detected by RT-PCR, and the products of RT-PCR were quantified by CE. Results The DNA fragments in the 100 bp DNA marker and the products of the RT-PCR were separated successfully by CE with the sieving buffer containing 1.8% hydroxypropylmethylcellulose (HPMC). It proved that there were no significant changes on the expressions of 5-LO mRNA when the cells were cultured for 72 h quantified by CE. The mRNA of 5-LO significantly decreased by almost 80% by CE with the cells cultured for 120 h in vitro.Conclusions The products of RT-PCR could be separated and quantified by CE directly.The 5-LO mRNA could express normally in the rat peritoneal macrophages for 72 h in vitro.

Objectives:In order to observe the effect of colonic carcinoma growth on amino acid metabolism. Methods:Levels of tumor tissue 20 free amino acids were measured from 18 patients with colonic carcinoma, and the normal colonic mucosa was used as controls.Reversed Phase High Performance Liquid Chromatography was used for the determination.The correlations between tumor volume and tumor tissue 20 free amino acids were analysed. Results:The contents of colonic carcinoma tissue free glutamine,methionine,arginine,threonine,valine,leucine,phenylalanine,aspargine and serine were significantly increased and the contents of tumor tissue free alanine and tryptophan were significantly decreased when compared with those of normal colon mucosa( P

Objective To study the significance of ?-catenin expression in human gastric carcinomas, colorectal carcinomas and human hepatic carcinomas.Methods S-P immunohistochemical method was used to detect ?-catenin expression in 80 cases of human carcinomas of alimentary system and corresponding tumor-adjacent normal tissues by ?-catenin polyclonal antibody. The results were analyzed using densitometrically semiquantitated .Results ?-catenin were normally expressed in all tumor-adjacent normal tissues of digestive system. ?-catenin expression was reduced or negative in human carcinomas of alimentary system (P

Objective To explore the efficacy of retroperitoneal laparoscopy in the treatment of parapelvic cyst.Methods From August 2005 to December 2008,16 cases of parapelvic cyst were treated by retroperitoneal laparoscopic deroofing and decompressing in our hospital.Under general anaesthesia,an operational space was established via retroperitoneal route.And then,along the urethra,the parapelvic cyst was explored.Afterwards,the cyst was unroofed 3 to 5 mm from the normal renal tissues.Results The operation was completed in 15 of the patients with a mean operation time of(75?20) minutes and mean blood loss of(28?8) ml.No injuries to the kidney pedicle or pelvis occurred.Another patient was converted to open surgery because of difficulty in exposure of the cyst.This series were followed up for a mean of 13 months(range,6 to 20 months).No one had recurrence during the period.Conclusions Retroperitoneal laparoscopic deroofing is minimally invasive,safe and effective or parapelvic cyst.It should be the first choice for the disease.

Objective To investigate the anti-inflammatory effect of proanthocyanidins and its mechanisms.Methods Inflammation models such as dimethylbenzene-indueed ear swelling and carrageenan-induced hind paw edema in mice and rats were prepared.The contents of PGE2 in exudate from edema paws of rats were measured by ultraviolet spectrophotography and the protein expression of COX-2 in edema paws of rats by Western-blot and immunohistoehemistry(IHC)assay.Results Pro-anthocyanidins remarkably inhibited the ear edema induced by dimethylbenzene in mice at the dose of10 mg/kg and 20 mg/kg;paw edema of rats induced with carrageenan was significantly inhibited byproanthocyanidins 5,20 mg/kg ip from 2 to 5 h;proanthoeyanidins 5,20 mg/kg ip reduced the contents of PGE2 in exudate from edema paws of rats induced by carrageenan;proanthocyanidins 5,20 mg/kg ip inhibited the protein expression of COX-2.Conclusion Proanthocyanidins has an anti-inflammatory effect in vivo which may be related to inhibition of protein expression of COX-2 and downregutation of PGE2 biosynthesis.

[Objective]To explore the dependability between heredity and hallux valgus.[Method]A total(1 491) cases of family heredity of hallux valgus patients were investigated,the ages of these patients,ages of onset,ages of aggravation,hallux valgus angles were analyzed statistically.[Result]Conclusive family medical history exists in 69.48% of the(1 491) hallux valgus patients.There was distinguished difference between the patients with family history and those without heredity background on visiting ages,ages of onset,ages of aggravation;but no any difference between these two groups of patients on hallux valgus angles.[Conclusion]Heredity factor is the main cause of hallux valgus.It can influence only the onset of hallux valgus,but not the severe degree of latter.The patients with family history are earlier than those without heredity background about visiting ages,ages of onset,ages of aggravation.

Objective To observe the effect of nursing intervention on type 2 diabetes in communities. Methods During Jul. 2007 to Jul. 2008, 256 cases with type 2 diabetes were chosen and intervened with non-medication treatment, direction for rational application of medication and management of the follow-up by community nurses. Results The awareness rates about blood presure, blood glucose, blood lipids and diabetes prevention & treatment among the patients were 47% , 43.8% , 60.7% and 91.5% . The compliance rates for medication, the treatment of non-medication and the attainment rate of self monitoring were 89%, 77.6% and 68%, respectively, and all of these results were significantly increased. Conclusion Nursing intervention on patients with Type 2 Diabetes in communities promotes the comprehensive treatment of diabetes.

BACKGROUND:The skin lesions, pathological and immunological characteristics of Nc/Nga mice are consistent with human atopic dermatitis, and as an atopic dermatitis animal model, it has great research value. But there are no reports on the physiological and biochemical parameters of Nc/Nga mice in China. OBJECTIVE:To observe the growth and reproduction and blood physiological and biochemical parameters of atopic dermatitis model of NC/Nga mice METHODS:The data of reproductive performance of NC/Nga mice from the first to the third generation was analyzed, including mean litter size, weaning rate, pregnancy rate and generation interval. The body mass of 60 Nc/Nga mice with 1-56 days old was measured, 30 mice of female and male, then the growth curve was draw. The blood samples from the infraorbital vessels were col ected to detect the blood physiological and biochemical parameters in the mice. RESULTS AND CONCLUSION:The mean delivery interval of Nc/Nga mice was (25.8±3.1) days, mean litter size was (7.5±2.5) mice, mean weaning rate was (97.2±1.2)%, the mean pregnancy rate was (97.0±1.4)%, and there was no significant difference among the three generations of these mice (P>0.05). The body mass of Nc/Nga mice was increased with the time increasing of days, the body mass of the mice was maximal within 1-2 weeks post-weaning, and there was no significant difference in body mass between males and females at 6 weeks post-weaning (P>0.05). Comparison of the blood physiological and biochemical parameters of mice at the same age between males and females showed that the levels of red blood cells and hemoglobin in female mice were significantly higher than those in the male mice;the platelet count in the male mice was significantly higher than that in the female mice (P<0.05);the triglycerides and albumin levels in the female mice were higher than those in the male mice (P<0.05). The results indicate that the gender and age may influence the blood physiological and biochemical parameters of Nc/Nga mice.