OBJECTIVE:To establish a method for the content determination of chloramphenicol and salicylic acid in Acne tinc-ture. METHODS:HPLC method was adopted with the column of Shim-pack CLC-ODS with the mobile phase of methanol-water (50∶50,V/V)at the flow rate of 0.8 ml/min. The detection wavelength was 278 nm,column temperature was 35 ℃,and the vol-ume was 10 μl. RESULTS:The linear range was 2.0-10.0 μg/ml(r=0.999 2)for chloramphenicol and 5.0-25.0 μg/ml(r=0.999 1) for salicylic acid;the RSDs of precision,stability and repeatability tests were lower than 1%;the average recoveries chlorampheni-col and salicylic acid were respectively 99.77%(RSD=0.35%,n=9) and 99.29%(RSD=0.48%,n=9). CONCLUSIONS:The method is simple,rapid,accurate,specific,precise,and can be used for quality control of Acne tincture.

OBJECTIVE:To provide baseline data of safe and rational drug use in pediatrics. METHODS:The pediatrics outpatient prescriptions were randomly selected from pediatric department of The Second Affiliated Hospital of Xinjiang Medi-cal University during Mar. 2014-Feb. 2015. The behavior of off-label drug use was determined according to the content of drug instruction. The type of off-label drug use,the relationship of different age groups with various drugs were analyzed. RE-SULTS:A total of 2400 outpatient prescriptions were collected from pediatric department,5115 medication records were ana-lyzed,involving 171 kinds of drugs. By the number of prescriptions,medication records and drug types,the incidence of off-la-bel drug use were 59.08%,40.87% and 80.70%,respectively. The main categories of drug off-label use were off-label dosing frequency(43.03%),off-label dosage(29.55%)and off-label indication(18.83%). The incidence of off-label drug use in in-fants and adolescents ranked the top 2 places,being 67.58%,63.01%. The top-four drugs in the list of the incidence of off-la-bel drug use were drugs for external use(55.56%),drugs for nerve system(54.12%),drugs for respiratory system(48.95%) and nutritional agents(48.09%). CONCLUSIONS:The phenomenon of drug off-label use is widespread in pediatric outpatient prescriptions.

OBJECTIVE:To reduce the risk of single dispensing,and to improve the quality of pharmaceutical care in emergen-cy pharmacy. METHODS:Single dispensing environmental factors,human factors and social factors of emergency pharmacy in our hospital were analyzed;the suggestions and measures were put forward,risk intervention mechanism was established and manage-ment effects were evaluated. RESULTS:Through improving the pharmacy hardware and software,carrying out the relevant sys-tem,enhancing training,improving the process of dispensing and window order,promoting the drug management and carrying out near error management. Compared with before management(Oct.-Dec. 2014),the times of near error and disputes significantly re-duced after management(Feb.-Apr. 2015)(P<0.05),and medication error reduced to zero. CONCLUSIONS:As single dispens-ing,risk management and the improvement of prescription audit ability can improve pharmaceutical care in emergency pharmacy.

Objective To analyze the effect of modified Tongyou decoction combined with FOLFOX4 chemotherapy on pre-thrombus state and curative effect of patients with advanced prostatic cancer.Methods 142 patients with advanced prostatic cancer were collected,all patients were randomly divided into study group and control group,71 cases in each group.The control group was given FOLFOX4 chemotherapy and the study group was given modified Tongyou decoction on the baseis of the control group.After treatment,the serum levels of homocysteine(Hcy),D-two dimer(D-D),fibrinogen(FIB),the level of hemorrheology,clinical effect,the incidence of venous thrombosis in one years,the six month survival rate,the one year survival rate were detected in all patients.Resutls After treatment,compared with control group,the serum levels of Hcy,D-D,FIB,the whole blood high,middle and low-shear viscosities,plasma viscosity and the incidence of venous thrombosis in one years were higher in the study group and the difference was statistically significant(P<0.05);the erythrocyte deformability index,effective rate and one year survival rate were higher were higher in the study group and the difference was statistically significant(P<0.05).Conclusion The modified Tongyou decoction combined with FOLFOX4 chemotherapy in treatment of patients with advanced prostatic cancer can improve the prethrombotic state,improve the therapeutic effect,reduce the incidence of venous thrombosis and increase the one year survival rate,and have a guiding significance for clinic.

Objective: To evaluated the granules stabilities in four conditions by taking the change of Baricalin content and granules surface color as stability test markers. Methods: The content of baicalin was determined by dual wave length iso absortion method and the granules surface color was determined by the diffuse reflectrance spectroscopy. ① The granules were tested in a simulated summer environment (30 ?C , RH92.5%, 1month) and in a high intensity light condition (3000LX, 10dyas). ② The influence of humidity on the stability of the granules was investigated. ③ The expiry date of the granules was predicted by the mutivariate linear regression analysis and compared with the result of the classical constant temperature method. ④ The influences of temperature and humidity on the granules stability were etudied by orthogonal test. Results: ① There was hardly any change in baicalin content of samples in a simulated summer environment and in a high intensity light condition. ② The results showed that the decrease of baicalin content and charge of granules color were obvious when the relative humidity of environment was high and the granules color was more easily influenced by the humidity. ③ The expiry date was about 2.87 years. ④ The results indicated that both temperature and humidity had effects on the change of baicalin content. Conclusion: We tried to estimate the stability of the granules by two indexes. The results showed that Qingrejedu granules was stable in different conditions. It satisfied the stability requirements of solid dosage form.

Animals ; Antigens, Nuclear ; metabolism ; Basic Helix-Loop-Helix Transcription Factors ; metabolism ; Biomarkers, Tumor ; metabolism ; Central Nervous System Neoplasms ; metabolism ; pathology ; Diagnosis, Differential ; Ependymoma ; metabolism ; pathology ; Humans ; Immunohistochemistry ; methods ; Intermediate Filament Proteins ; metabolism ; Medulloblastoma ; metabolism ; pathology ; Mucin-1 ; metabolism ; Nerve Tissue Proteins ; metabolism ; Nestin ; Neurocytoma ; metabolism ; pathology ; Oligodendrocyte Transcription Factor 2 ; Oligodendroglioma ; metabolism ; pathology

Antigens, Neoplasm ; metabolism ; Antigens, Surface ; metabolism ; Autoantibodies ; metabolism ; Biomarkers, Tumor ; metabolism ; Carrier Proteins ; metabolism ; Glutamate Carboxypeptidase II ; metabolism ; Humans ; Kallikreins ; metabolism ; Male ; MicroRNAs ; metabolism ; Oncogene Proteins, Fusion ; metabolism ; PTEN Phosphohydrolase ; metabolism ; Prostate-Specific Antigen ; metabolism ; Prostatic Neoplasms ; metabolism ; Receptor, Epidermal Growth Factor ; metabolism ; Trypsin Inhibitor, Kazal Pancreatic ; Urokinase-Type Plasminogen Activator ; metabolism

Aneuploidy ; Gene Rearrangement ; Glutathione S-Transferase pi ; metabolism ; Humans ; Male ; Methylation ; Neoplasm Grading ; Neoplasm Staging ; Neoplasms, Multiple Primary ; genetics ; metabolism ; pathology ; surgery ; Oncogene Proteins, Fusion ; genetics ; Prostate-Specific Antigen ; metabolism ; Prostatic Intraepithelial Neoplasia ; genetics ; pathology ; Prostatic Neoplasms ; genetics ; metabolism ; pathology ; surgery

Objective To explore the changes of plasma orexin-A level before and after operation in obstructive sleep apnea-hypopnea syndrome(OSAHS) children and its effect on their behavior performance.Methods 120 cases OSAHS children performed tonsillectomy and / or adenoidectomy and 30 cases normal children as control group.According to the AHI index,the OSAHS group was divided into mild group (5 times/h ≤ AHI ＜ 20times/h,n=13),moderate group (20/h ≤ AHI ＜40/h,n=76),and severe group (AHI ≥ 40 times / h,n=31).And at the same time,according to the sensory integration ratings,OSAHS group was divided into normal group(n =30),mildly abnormal group (n =47),moderately abnormal group (n =28),severely abnormal group (n =15).Before operation and 6 months after operation,plasma orexin-A levels and children's sensory integration were measured.Results Plasma orexin-A level of the OSAHS group ((0.41 ± 0.06) μg/ml) was significantly higher compared with the control group((0.31±0.04) μg/ml) (P＜0.01).In orexin-A level of different AHI groups before and after operation(mild group:(0.33±0.02) μg/ml vs (0.28± 0.03) μg/ml,moderate group:(0.39±0.04) μg/ml vs (0.29±0.03) μg/ml,severe group:(0.49±0.04) μg/ml vs (0.32± 0.02) μg/ml),there had significant differences (P＜0.01).In OSAHS children,AHI index had positive correlation with preoperative plasma orexin-A level (r=0.803,P＜0.01).There was a significant negative correlation between sensory integration scores and plasma orexinA level(r=-0.812,P＜0.01).Conclusions Plasma orexin-A level of OSAHS children is closely related to the severity of OSAHS and the changes of their behavioral ability.And it may become a diagnostic plasma marker of OSAHS children.

Objective To study the clinical and genetic characteristics of keloid through investigating on four Han Chinese pedigrees.Methods The pedigree information and clinical data from Han Chinese keloid pedigrees were collected,which consisted of 22 patients in 127 family members,and then the charts of these pedigrees were constructed according to the data.Using the genetic model and pedigree analyses we summarized the clinical features of the disease in the families.Results Four Han Chinese keloid pedigrees were discovered.The three pedigree spans included 3 generations and one was 4 generations.Incidence of KD in the consanguinity family member was 23.7％ (23/93),and 20.8％ (11/53) in male KD,and 27.5％ (11/40) in female.Incidence of anterior chest KD was 40.9 ％.The inheritance pattern observed in these pedigrees was consistent with an autosomal dominant inheritance multi-gene hereditary disease with incomplete penetrance,and its nonpenetrance of KD gene carriers was 12％ (3/25).Conclusions The pattern of inheritance observed in these four Han Chinese keloid pedigrees is similar to previous reports and no gender differences are found in the incidence of disease,but differences in pathogenic site.Pedigree investigation helps to reveal the genetic characteristics of keloid.