AIM:To investigate the effect of Gln deprivation on growth and differentiation of primary APL cells. METHODS: The primary APL cells were collected from 18 cases of APL patients' periphery blood, the patients had not been treated. The cells were incubated in RPMI 1640 without or with different contents of glutamine and with 10% fetal calf sera, at 37℃,5% CO 2 and saturation humidity for 4 days.The initial living cell density was 5?10 8/L. After 4 days incubation,the cells were counted,collected, smeard and stained with Wright-Giemsa, DNA, POX, NAE and NaF inhibition,gulping ink and NBT etc. cytochemical technology. The stained cells were investigated under oil immersion lens. RESULTS: The living cell density became (54.28?4.28)% of the initial in glutamine deprivation group, but the living cell density in control with 4 mmol/L Gln was (108.56?12.27 )% of the initial ( P

Cardiac Catheterization ; methods ; Heart Defects, Congenital ; therapy ; Humans

Enteral Nutrition ; methods ; Humans ; Intestines ; pathology ; physiopathology

Animals ; Burns ; drug therapy ; metabolism ; Glutamine ; pharmacology ; therapeutic use ; Humans ; Wound Healing ; drug effects

Objective To observe the occurrence of hyperhomocysteinemia among patients suffering from cerebral infarction,and explore the possible risk factors.Methods Data of 144 cerebral infarction patients were collected from a tertiary hospital in Tianjin including gender,age,diabetes mellitus,hypertension,smoking,alcoholism,plasma homocysteine,HbAlc and biochemical criterion.Two groups were created as the hyperhomocysteinemia group and the control group based on serum homocysteine levels.Risk factor for hyperhomocysteinemia was analyzed by application of Logistic regression.Results Logistic regression analysis revealed that hyperhomocysteinemia was associated with gender,smoking,drinking and HbAlc levels in patients.Conclusions Male,smoking,drinking and diabetes mellitus were the possible risk factors in cerebral infarction patients.

Arginine ; therapeutic use ; Burns ; therapy ; Humans ; Nutrition Therapy ; Wound Healing

Burns ; Periodicals as Topic ; Surgery, Plastic

Animals ; Anti-Inflammatory Agents ; pharmacology ; Cyclooxygenase 2 ; metabolism ; Inflammation ; metabolism ; Insulin ; pharmacology ; Nitric Oxide Synthase Type II ; metabolism ; RNA, Messenger ; genetics ; Rats

OBJECTIVE: Cerebral vasospasm (CVS) induced by subarachnoid hemorrhage (SAH) is a difficulty in neurosciences. Because the pathogenesis of CVS remains a matter of discussion, clinical prevention and treatment are currently not sufficient. With the application of advanced experimental technology in recent years, great breakthroughs have been achieved in domestic and foreign studies on potassium channels. Definite mechanism in the region plays a significant role in instruction for clinical prevention and treatment. In this paper, researches related to potassium channels in cerebral vascular vessels after SAH are reviewed.DATA SOURCES: Related articles published between January 1991 and December 2005 were searched by the computer in Medline database with the key words of "potassium channels, subarachnoid hemorrhage, cerebral vasospasm" and the language was limited to English. Meanwhile, relevant articles were also searched in CNKI and Wanfang database with the same key words in Ghinese.STUDY SELECTION: All articles were selected firstly, and literatures related to potassium channel on physiology and pathogenesis of SAH-induced CVS were selected. Researches on biochemistry and gene etc were excluded.DATA EXTRACTION: A total of 45 English articles were collected, among which, 17 met the inclusion criteria. Of 2 Chinese articles collected,one study was in accordance with the inclusion criteria. Eighteen literatures on the pathological and physiological mechanism of CVS after SAH were looked for the full text.DATA SYNTHESIS: Researches showed that remarkable changes occurred in potassium channels in CVS after SAH, while potassium channel modulators had significant interventional effect on CVS in animal experiment by promoting or blocking the ion movements.CONCLUSION: Potassium channels plays an important role in the pathogenesis of SAH-indueed CVS. Some therapeutic intervention may be provided based on the studies of potassium channels in CVS.

Non-communicable-diseases(NCDs)have become leading death causes and major health challenges for the Chinese people,incurring direct and indirect economic burden of diseases on families and the society,and even posing a serious threat to social-economic development sustainability of the country.Given their complex causes,NCDs mostly result from unhealthy lifestyle of the individuals,along with complex multiple social determinants.Evidences from home and abroad prove NCDs as preventable and controllable,which call for powerful government policy commitments and leadership.The government should make health policies part of social policies,build such intervention platform as Healthy City,rebuild the health service system,promote universal coverage of cost-effective interventions,and involve the entire society,reinforce general measures such as planning and performance monitoring.All of which can achieve desired outcomes for NCD control in the end.