Objective:To study the the mental health and relevant factors of patients with hepatitis B. Methods: 58 cases of patients with hepatitis B were assessed with the Social Support Scale, Symptom Checklist-90 (SCL-90), Trait Coping Questionnaire and compared with healthy person. Results: All patients had obvious psychosomatic symptoms. The mental health level was negatively correlated with postive coping style and social support. Conclusion: Several psychosocial factors played an important role in the onset and trealment of Hepatitis B disease.

Objective To compare the neurotoxic effects of intratheeal ropivacaine and bupivacaine on the spinal cord in rats.Methods Female SD rats weighing 240-330 g were used in this study.Catheters(PE 10 tubing)were inserted through an incision in the cisternal membrane and advanced caudad until the tip of the catheter reached the lumbar spinal level according to the technique described by Yaksh and Rudy.Fifty-four successfully catheterized rats were randomly assigned tO one of 3 groups(n=18 each):group Ⅰ received normal saline 30μlIT(NS);groupⅡ received 2%bupivacaine 20μl IT(BU)and group Ⅲ 2.7%ropivacaine 20μl IT (RO).Degree of motor block was assessed and scored(0=no block,2=inability to flex the hind limb)before (T1,baseline)and at 10,20,30,60 and 120 min(T2-6)after IT injection.On the 4th day after IT injection specimens were obtained from lumbar spinal cord for microscopic examination.Results The motor block score was significantly higher in group BU and RO than in group NS(P＜0.05),and the tissue damage was severe in group BU and RO.The motor block scores were significantly higher at T2 than at T3-6 in beth group BU and RO(P＜0.05).There Was no significant difference in motor block score at T2 between groups BU and RO but the motor block scores were significantly lower at T3-5 in group RO than in group BU(P＜0.05).The tissue damage was Severer in group RO than in group BU(P＜0.05).Conclusion Intrathecal 2.7% ropivacaine is more toxic to the spinal cord than intrathecal 2%bupivacaine in rats.

Objective To construct directional expressing cDNA library from normal testes in Chinese males for further studying the structure and functions of spermatogenesis related genes. Methods mRNA of normal testes from a Chinese male was isolated and the cDNA synthesized by reverse transcription.After being digested by SalI and NotI ,dscDNA was subcloned into expressing plasmid pSPORT1 directionally.Finally,the directional expressing cDNA library from normal testes in Chinese males was constructed. Results The transformation rate of the library was 7?10 5.Ten clones were chosen and analysed. Digested by SalI and NotI ,the fragments of 0.5～2.0 kb were obtained with cDNA inserted. Conclusions The present study indicates that the directional expressing cDNA library from normal testes in Chinese males is qualified to screen genes and is considered to be helpful for studying the structure and functions of spermatogenesis related genes.

Objective To investigate the condition of microdeletions of AZF region of the Y chromosome in Chinese patients with spermatogenetic malfunction. Methods Six Y chromosome specific sequence-tagged-sites(STS) in AZF region were screened and the microdeletions were determined by PCR in 27 patients with spermatogenetic malfunction. Results Microdeletions in genomic DNA were observed in 2 cases who presented with azoospermia and limited to AZFc subregion.The total deletion rate was 7.4%.The deletion patterns of the 2 cases were DAZ(sY254,sY255) and DAZ plus sY157,respectively. Conclusions Like other races, microdeletions of the Y chromosome in Chinese people may be one of the causes of spermatogenetic malfunction.Therefore prior to assistant reproduction,screening for microdeletions of the Y chromosome in patients with spermatogenetic malfunction is necessary.

To investigate the interrelationship between plasma renin activity(PRAXaldosterone (Ald),atrial natri-uretic peptide ( ANP ) and blood volume ( BV ) in adult nephrotic syndrome ( NS ) . Methods BV was assessed using 113mInCl dilution method. PRA、 Angiotensin Ⅱ (AngⅡ)、Ald、ANP were determined by radioirnmunoassay. 28 edema-tous NS patients,26 healthy controls and 18 cases of remission were involved. Results (1)During edematous period, PR, AngⅡ , Ald、 ANP were higher in NS than in controls. There was no difference of BV between NS and controls. Albumin ,PRA,AngⅡ 、Ald and ANP were not correlated with BV; (2)After remission, comparing with edematous period, Ald 、ANP deceased significantly whereas PRA、AngⅡ、Ald and total BV were not different; (3) Urine natrium excretion (UNaV) was negatively correlated with Aid. Conclusion Ald and ANP may be the major regulating factors of sodium excretion in nephrotic syndrome.

An analysis of the present development of general practitioners in China,probed into the bottlenecks found.The paper proposed to set forth from the training,use and incentives of GPs to promote the systematic design of China's GP system.These measures center on the regulation of GPs development,while building a multi-channel and multi-level GP development system.Other recommendations include raising the professional standing and attractiveness of GPs as a discipline,and enhancing GPs capacity building to promote general practitioners development.

Objective To study changes in expression of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA in the kidney and spleen of dead rats and its application in deducing the postmortem intervals (PMI), so as to look for a new method for PMI deduction. Method 28 rats were killed by cervical dislocation, placed in a chamber with temperature-controlling system at 20℃ for 0 to 48h postmortem, and autopsied at 0, 8, 16, 24, 32, 40 and 48h respectively (n=4 for each time point). The expression of GAPDH mRNA in kidney and spleen was assessed with fluorescence semi-quantitative RT-PCR. Results The amplified products of GAPDH mRNA could be detected in kidney within 40h and in spleen within 32h postmortem respectively, and they showed a decreasing tendency as its fluorescence intensity declined gradually and lost at last. Conclusion Detection of GAPDH mRNA expression in kidney and spleen of rats at different periods after death by fluorescence semi-quantitative RT-PCR seems to be of some value for estimation of PMI.

30ml) undergoing minimally invasive evacuation of intracranial hematoma in our department were analyzed retrospectively.The chants of IL-1,IL-6 and IL-8 in hematoma fluid were observed continuously.The content of IL-1,IL-6 and IL-8 was determined by radioimmunassay.Results IL-1,IL-6 and IL-8 were observed at 6～12h after hypertensive cerebral hemorrhage,and showed different changes in acute stage.Conclusion Inflammatory cytokines are involved in the pathological process of cerebral hemorrhage.

[Objective] To investigate the effects of Qianggu capsule plus alendronate on postmenopausal osteoporosis.[Methods]By random number generating method,120 patients were randomly divided into a treatment group with Qianggu capsule plus alendronate,control group A only with Qianggu capsule,and control group B only with alendronate.The pain of the patient before treatment,3 m and 6 m after treatment was assessed.At the same time,the bone mineral density (BMD) before and 6 months after treatment was recorded.[Results] There was significant difference before and after 3 m in the score of pain in three groups,but the difference in treatment group was higher than other two control groups.After 6 m,all the scores of pain in 3 groups had changed significantly compared to the ones before treatment,but the scores of pain in treatment group were lower than 2 control groups.The BMD of both vertebral and ward in treatment group after 6 months treatment was higher than before.[Conclusions]Qianggu capsule plus alendronate has definite therapeutic effects on postmenopausal osteoporosis.

Objective To study difference of coronary artery lesion between Han nationality and Uygur nationality patients with coronary artery disease .Methods 2008 patients with coronary artery disease were analyzed retrospectively in our hospital .The cor-onary artery lesion was evaluated by the number ,location ,type of coronary lesion and Gensini scores .Results The incidences of three-vessel disease in Uygur patients (40 .6% ) were higher than that of Han patients (35 .3% ) .Along with the age increasing , Gensini scores increased gradually .Gensini scores of uygur patients were significantly higher than those of Han patients in same age groups .Compared with the Han patients ,mean age in the Uygur patients was lower(P<0 .05) .Conclusion Severity of coronary artery disease increased with age ,single-vessel lesion gradually reduced and three-vessel lesion gradually increased .The coronary artery lesions of Uygur patients were more severe than those of Han patients in same age :more three-branch lesion .Compared with Han patients ,morbidity age of similarly degree coronary atherosclerosis in the Uygur patients maybe more early .