Objective To compare the neurotoxic effects of intratheeal ropivacaine and bupivacaine on the spinal cord in rats.Methods Female SD rats weighing 240-330 g were used in this study.Catheters(PE 10 tubing)were inserted through an incision in the cisternal membrane and advanced caudad until the tip of the catheter reached the lumbar spinal level according to the technique described by Yaksh and Rudy.Fifty-four successfully catheterized rats were randomly assigned tO one of 3 groups(n=18 each):group Ⅰ received normal saline 30μlIT(NS);groupⅡ received 2%bupivacaine 20μl IT(BU)and group Ⅲ 2.7%ropivacaine 20μl IT (RO).Degree of motor block was assessed and scored(0=no block,2=inability to flex the hind limb)before (T1,baseline)and at 10,20,30,60 and 120 min(T2-6)after IT injection.On the 4th day after IT injection specimens were obtained from lumbar spinal cord for microscopic examination.Results The motor block score was significantly higher in group BU and RO than in group NS(P＜0.05),and the tissue damage was severe in group BU and RO.The motor block scores were significantly higher at T2 than at T3-6 in beth group BU and RO(P＜0.05).There Was no significant difference in motor block score at T2 between groups BU and RO but the motor block scores were significantly lower at T3-5 in group RO than in group BU(P＜0.05).The tissue damage was Severer in group RO than in group BU(P＜0.05).Conclusion Intrathecal 2.7% ropivacaine is more toxic to the spinal cord than intrathecal 2%bupivacaine in rats.

Objective: To investigate the CT and MRI findings of solid-pseudopapillary tumors of the pancreas(SPTP),and to improve the diagnosis of the disease.Methods: Three female SPTP patients received CT examination,1 of them underwent MRI in addition,and all the cases were surgically and pathologically confirmed later.Results: CT images revealed huge solid-cystic mass in the pancreas of the 3 patients,1 with calcification in the mass and all without dilation of the bile duct.MRI exhibited mixed signal intensity on T1WI and T2WI.Both CT and MRI showed obvious enhancement of the cystic wall and the solid part of the mass.Conclusion: SPTP has its characteristic imaging manifestations,which may contribute a lot to the diagnosis of the disease.

Cognition ; Language ; Medicine, Chinese Traditional ; Meridians ; Metaphor ; Translations

Objective To observe the expression of pancreatic K_(ATP) channels (Kir6.2/SUR1) in rats with chronic pancreatitis and explore the intervention of nateglinide on the changes.Methods Wistar rats were induced to suffer from chronic pancreatitis and then randomized into model group, nateglinide group and control group.Then OGTT of them were observed.RT-PCR was used to detect the expression of Kir6.2 and SUR1 mRNA, and western blot to detect the expression of Kir6.2 and SUR1 proteins.Results Model rats displayed impaired glucose tolerance (IGT).The expression of Kir6.2 and SUR1 in model group decreased significantly(P＜0.05), and nateglinide displayed up-reg-ulation to the expression in some degree.Conclusion The expression of pancreatic K_(ATP) channels in rats with chronic pancreatitis diminished, which might be the important mechanism of the development of pancreatogenic diabetes.Nateglinide can up-regulate the expression in some degree, which indicates that it may have latent effect of ameliorating the prognosis of patients with chronic pancreatitis.

0.05),including seven with spinal kyphosis,seven with thoracic deformity,five with pleural effusion,five with emphysema diagnosed by chest X-ray film,three with spontaneous pneumothorax,two with diaphragmatic hernia,and one developed severe respiratory failure and the other developed cot pulmonale.Conclusions The thorax,lungs and diaphragm were more easily involved in the patients with Marfan syndrome,and pulmonary specialists should keep an eye on them.

Dual source CT is a kind of 64 slices CT including two acquisition systems. It possesses such advantages as higher SNR of images,higher resolution of time,lower dose of radiation,bigger FOV of scanning,wider coverage of scanning and dual enrage subtraction imaging,etc. Weather these advantages could be brought into full play has great relationship with the preparations of dual source CT before its installation. The corresponding discussion is made in this text to see how to make preparations for dual source CT in different aspects such as room building,surrounding background,power supply,fire protection,radiation protection and periphery,etc.

Objective To observe the value of the corresponding solutions for MRI static magnetic field artifacts after analyzing their generating mechanism.Methods Different solutions was used to eliminate static magnetic field artifacts,such as improving inner and outer background,calibrating software and hardware,editing parameters,instructing patients,etc.in scanning patients or phantom with Siemens Impact 1.0T and GE Signa 1.5T MR by the use of SE or GRE sequences.Results Static magnetic field artifacts were controlled to different extent and the quality of the mass MR images were improved.Conclusion Most static magnetic field artifacts can be eliminated completely or reduced by using corresponding solutions,so more corrective information can be provided to clinic.

Objective To optimize the processing technology of stir-frying with vinegar of Curcuma Longa Radix (CLR) by orthogonal design and Box-Behnken design-respanse surface method (BBD-RSM) based on entropy method. Methods As comprehensive evaluation indexes, the contents of curcumin, demethoxycurcumin, and bisdemethoxycurcumin in CLR processed by traditional method were determined by HPLC. The orthogonal test was adapted to examine the influence of the amount of vinegar, the moistening time, parching time, and parching temperature on processing technology of stir-frying with vinegar. Based on the results above, BBD-RSM was adopted to optimize the processing technology further using the moistening time, parching time and parching temperature as factors. Results The optimum processing technology of the orthogonal test was covered the amount of vinegar of 15%, moistening time of 10 min, parching temperature of 130 ℃, and parching time of 10 min. The optimum processing technology by BBD-RSM was covered moistening time of 12 min, parching temperature of 150 ℃, and parching time of 8 min. The verification esting indicates that the process conditions are reasonable and feasible with good reproducibility. Conclusion The method and data are precise and reliable. Besides, it established the processing technology of vinegar CLR and provided a theoretical basis for the processing technology of stir-frying with vinegar of CLR.

Objective To investigate the clinical pathological factors of papillary thyroid microcarcinoma (PTMC) with clinically node-negative sides of neck stage central lymph node metastasis.Methods Analysis of 136 cases of cNo stage of PTMC of clinical and pathological data,and using the PCR method for the detection of BRAFV600E mutation in the paraffin coated tissue.Results One hundred and thirty-six cases with PTMC lymph node metastasis rate was 38.2％ (52/136),BRAFV600E mutation rate was 44.9％ (61/136).Single factor analysis showed that the lymph node metastasis was related with BRAFV600E mutation and capsular invasion (P＜ 0.05).The size of the tumor was close to significant level (P=0.057).Multiple regression analysis showed that BRAFV600E mutation and capsular invasion were independent factors for affecting the central lymph node metastasis (P ＜ 0.05).Conclusion The routine central lymph node dissection should be performed in patients with BRAFV600E mutation and tumor invasion.

Objective To evaluate the safety and feasibility of totally thoracoscopic anatomic pulmonary segmentectomy (TTAS) for the treatment of the peripheral stage ⅠA non small cell lung cancer(NSCLC).Methods The study involved 50 consecutive patients undergoing totally thoracoscopic anatomic segmentectomy (TTAS) from September 2010 to November 2012 in the First People's Hospital affiliatied to Nanjing Medical University.The diameter of the tumors were less than 2 cm [(mean diameter(1.35 ±0.48) cm].All lymph node sampling of N1 and N2 were neglive,All patients received symtematic lymph node dissection.The pulmonary vessels were individually ligated,and the bronchi were closed using an endoscopic stapler.The intersegmental plane was identified using the demarcation between the resected(inflated) and preserved(collapsed) lungs.Staplers were used for intersegmental dissection.Results The mean operative time and intraoperative bleeding were (191.5 ± 50.4) min and (49.2 ± 54.6) ml respectively.The chest tube drainage duration was (3 ± 1) days.The number of stapler cartridges used for intersegmental division was 3.9 ±0.8.The mean number of lymph nodes and nodal stations dissected were 12.6 ± 2.8 and 6.0 ± 1.5 respectively.No mortality and complications were observed 30 days after the surgery.Further,no local recurrence or metastases were observed during follow-up.Conclusion Totally thoracoscopic anatomic segmentectomy(TTAS) is a feasible and safe technique.With systematic lymph node dissection,TTAS can be a reasonable therapeutic option for stage ⅠA NSCLC.