The effect of TMY compound fibrinolytic agent separated from T. muctosqua matus ven- om on acute thrombotic stroke of cat model has been studied. The animal model of acute thrombotic stroke was obtained by exposing MCA transorbitally and inserting a copper wire into it. Intracarotid small dose continuous infusion of thrombolytic agents were used in the treatment of thrombotic stroke. The urokinase treatment group was for the positive control group. MCA thrombotic formation and recanalization after thrombolytic agents infusion were detected by DSA. FIB and WBPA rate were tested before and after thrombolytic agents infusion to explore the fibrinolytic mechanism of these agents. One week altar thrombotic for- mation, all the animals were CT scanned to test the infarction volumes ad intracranial bleed- ing. In the saline group, there were no recanalization within 24 hours and a define region of cerebral infarction voiume (32?9.6%) wets detected at one week after thrombotic forma- tion, In both TMV group and urokinase group, diminution of infarction volume (26.1 ? 8.8, 26.5? 9.4% ) and increase of recanalization rates ( 5/ 6, 6/ 7) were produced. Furthermore in both fibrinolytic treatment groups, system fibrinogenolysis and inhibition of platelet aggregation were produced after fibrinolytic agents infusion.

Objective To study the clinical efficacy and safety of pereutaneous coronary intervention (PCI) via radial artery in the elderly patients with coronary heart disease complicated by chronic left heart failure. Methods120 cases with coronary heart disease complicated by chronic left heart were divided into two groups: group via radial artery (RA) (64 cases) and group via femoral artery (FA) (56 cases).The exposure time of X-ray,the time lying in bed after operation and the complication rates were retrospectively analyzed.ResultsThere were 60 cases via RA-and 54 cases via FA-PCI treatment,75 and 63 lesions successfully expanded,including 38 and 36 cases of anterior descending,19 and 12 cases of circumflex branch,18 and 15 cases of right coronary artery,73 and 61 stent implanted in radial artery group and femoral artery group,respectively.The difference of treatment and pathological change between the two groups was not statistically significant.The time from puncture to reset tube was longer,average time lying in bed was shorter,and acute left heart failure less occurred in radial artery group compared with femoral artery group (all P＜0.01).The puncture complications and rates of lower limb deep vein thrombosis,and lung embolism were lower in radial artery group than in femoral artery group (all P＜0.05).The incidence rates of pain in back and loin,dysuresia and abdominal distension were higher in femoral artery group than in radial artery group(P＜0.05).Conclusions PCI shows more advantages via radial artery versus via femoral artery in treatment of the elderly coronary heart disease complicated with chronic left heart failure.

Congenital pyriform sinus fistula (CPSF) is a rare branchial abnormality,presented as acute suppurative thyroiditis, recurrent neck abscess and cellulitis.In the past, the only way to cure CPSF is surgical resect of the whole lesion in the inflammation quiescent period, which always lead to many complications and high rate of recurrence.Since late 1990s,different kinds of endoscopic procedures have beeen attempted to close the inner orifice of congenital pyriform sinus fistula.After more than 10 years,endoscopic cauterization has been recommended as the first-line treatment for its safety, effective and minimally invasive.This paper introduces and summarizes the theory, procedure, advantages and disadvantages, indications and complications of endoscopic treatment of congenital pyriform sinus fistula.

Animals ; Burns ; immunology ; Humans ; Wound Healing ; immunology

OBJECTIVE:To control the drug quality risk in drug withdrawal links of hospital pharmacy,and provide reference for the application of quality risk management(QRM)in hospital pharmacy. METHODS:Drug QRM was developed in drug with-drawal links through risk identification,risk assessment,risk control,risk assessment and other steps. Using the incidence of risk factors and reusable rate of withdrawal drug as indexes,related data of before(Jul.-Dec. 2015)and after(Jan.-Jun. 2016)devel-oping QRM in our hospital were compared to evaluate the effect of drug QRM. RESULTS:Determining whether specific storage drugs kept the required storage conditions,whether the numbers of withdrawal drugs counted to minimal packaging and other 5 fac-tors were high-risk factors(the risk score of each factor>4 points);control measures were taken separately for high risk factors, then high-risk factors were reduced to acceptable levels(risk score<4 points). After developing QRM,the incidence of risk factors were reduced than before(reduce 1.35%-6.19%),reusable rate of withdrawal drug was increased(98.64% vs. 86.32%)(all P<0.05). CONCLUSIONS:Developing QRM in drug withdrawal links of hospital pharmacy can reduce the drug quality risk.

Objective: To design one kind of medical infusion pump. Methods: Based on single-chip microcomputer, 89C52, use the stepper motor to drive the semi extrusion micro flow pump. Results:During the infusion it can real-time monitor the block and bubble in the pipeline and alarm. It can accurately control the transfusion speed and infusion volume. With friendly man-machine interface it can display real-time infusion parameters and alarm signals. Conclusion: The infusion pump system has high precision and reliability, perfect function, and it is easy to use. Clinical practices show that it can effectively reduce the workload of the clinical nursing staff and improve work efficiency. It has high application value.

AIM: To study the effect of carbon monoxide (CO) on hypoxia inducible factor-1? (HIF-1?) expression and on hypoxic tolerance duration in hypoxia preconditioned mice. METHODS: Mice were injected with normal saline (NS) or hemin intraperitoneally. Western blot was used to detect HIF-1? in hippocampus. The tolerant duration of each hypoxic run was recorded. RESULTS: After 1 and 2 runs of hypoxia exposure, there were no significant differences in HIF-1? expression and hypoxic tolerance duration between N and hemin injection groups. After 3 and 4 runs of hypoxia exposure, the content of HIF-1? was lower and hypoxic tolerance duration was shorter ( P

Objective To investigate the expression of p 120-catenin in human bladder cell line BIU-87 and T24.And to explore the effect of down-regulation of p120-catenin on proliferation, invasion and adhesion of BIU-87 cell line. Methods The study was from Aug .2010 to May.2011.Real-time RT-PCR and Western blot were used to examine the expression of p 120-catenin in human bladder cell line BIU-87 and T24.There were three study groups:non-transfected group , the group transfected with p 120-catenin siRNA and the group transfected with control siRNA .p120-catenin siRNA was used to decrease the expression of p120-catenin.MTT assay and colony formation assay were used to study the BIU-87 cell growth and cell pro-liferation.The invasion of BIU-87 cells was measured by Transwell chamber assay .Cell adhesion artificial re-constituted basement membrane assay was used to examine the change of BIU-87 cell adhesion capacity . Results In both BIU-87 and T24 cells there were the expressions of p 120-catenin.But the expression in BIU-87 cells (0.11±0.39) was more than that in T24 cells (0.48±0.17).The decreased of p120-catenin ex-pression could enhance the proliferation (182.7±13.4%) and the invasiveness (217.5±15.9) and decrease the adhesion capacity (57.3±6.4%) of BIU-87 cells. Conclusions There is higher expression level of p120-catenin in lower-grade malignant bladder cancer cells .The down-regulation of p120-catenin promotes the bladder cancer proliferation and invasiveness of bladder carcinoma cells , and inhibited the bladder canc-er cell adhesion .

In recent years,hepatocellular carcinoma (HCC)has become the second leading cause of cancer-related death worldwide.Due to the insidious onset of this disease,only 20%-30%of the patients with HCC have the opportunity for surgical resection or liver transplan-tation.At present,interventional therapy has become the first choice for patients with intermediate-stage HCC and some patients with late-stage HCC,and more and more scholars have realized the importance and necessity of comprehensive interventional therapy for HCC.The comprehensive interventional therapy of transcatheter arterial chemoembolization (TACE)in combination with various methods (TACE com-bined with local treatment,TACE sequential surgery,or TACE combined with systemic treatment)provides many therapeutic approaches and achieves good therapeutic effects.However,the indications,timing,and recurrence and metastasis after treatment for such combination ther-apies await further investigation.

Objective To explore the relationship of hardiness,stress and psychological symptoms.Methods 622 college students participated the present study.Hardiness Scale,Chinese College Student Psychochological symptoms(r=-0.240,P＜0.01).Stress had significantly positive correlation with psychological sympefficient of interaction between stress and hardiness had significant predictability on psychological symptoms(△R2=0.041,P＜0.01).Conclusion Stress had a significant predictability on psychological symptoms.Hardiness was a moderator on stress and psychological symptoms,which can relieve adverse effects induced by stress.