Objectives To study the liver protective effect of L arginine for postoperative cirrhotic patients with primary liver cancer (PLC) receiving total parenteral nutrition (TPN). Methods Thirty preoperative cases were divided into two groups:Group A,TPN only( n =14);Group B,TPN+arginine( n =16). Liver function, serum lipid, immune function and serum nitric oxide were observed on preoperative phase, postoperative day 1 (POD 1) and day 7 (POD7) respectively. Liver biopsy was taken for histological study intraoperatively and on POD 7. Results Compared with group A on POD7,AST and TBIL level were near preoperative level, ALB level signiflcanly decreased, TG was lower than preoperative level, CD4 + and NO increased signiflcanly,and the degree of liver steatosis showed a signiflcant decrease in group B. Conclusions Supplement of appropriate amounts of L arginine increases hepatic NO level, reduces serverity of hepatic steatosis associated with TPN, promotes postoperative recovery of transaminase activity, ameliorates cell mediated immunity and anti infection capacity in postoperative PLC patients.

Objective To study the effect of endoscopic treatment in acute severe pancreafitis. Methods EST(endoscopic sphincterepapiilotomy) and ENBD ( endoscopic naso-billary dralnage) or ENPD ( endoscopic naso-pancreatic drainage) were used to treat the acute severe pancreatitis(ASP). Results The recover time of blood dia-stase of the endoscopic therapy group and the contrast group was (3.2±1.5) days, (6.6±1.2) days, respectively (P<0.01) ; the incidence of needing to be operated was 0.7% (1/140), 6.5% (8/124), respectively (P<0.05) ;mortality rate was 0:7% (1/140) ,7. 3% (9/124) ,respectively(P <0. 01 ) ;the incidence of complication was 3.6% (5/140) ,41.9% (52/124), respecfivly(P<0.01). Conclusion EST + ENBD(or ENPD) in treating ASP has cer-tain effect and is the primary therapy to the ASP.

Objective To study the use on curative effect of Chinese medicine Qingyitang in severe acute pancreatitis.Methods 132 cases of severe acute pancreatitis were randomly divided into 2 groups,The control group(n=62) and the treatment group(n=70).Routine treatment were given in both,otherwise,the Chinese medicine Qingyitang was used in the treatment group.The operation rate,the infection rate,the time of abdominal distension removing and fasting and being hospitalized were observed and compared between two groups.Results Every index of the treatment group was finer than the control group.Conclusions The Chinese medicine Qingyitang showes their protective effects on gut barrier function by alleviating the damage of intestinal mucosa and microecologic disturbance following acute pancreatitis.As a result,the chances of bacterial translocation and enterogenic infection declines.These preparation might raise the treatment effects,as a well,shorten the course of treatment.

Objective To investigate the effects of urotensinⅡ(UⅡ)receptor antagonist(URA)on UⅡ in plasma and bronchoalveolar lavage fluid(BALF)in rats with acute lung injury(ALI).Methods Twenty eight Sprague Dawley(SD)rats were randomly divided into four groups:saline control group(group A),the other three groups(group B,group C,group D).Rats were injected with oleic acid and URA to produce ALI models.Three hours after injection,arterial blood was drawn for blood gas analysis in all rats.Immediately after this,blood of heart was collected in group A and group B.Then blood of heart were collected after 12 hours in group C and after 24 hours in group D.Rats were killed when blood of heart was drawn.Bronchoalveolar lavage(BAL)with saline was performed in the right lung.BALF was centrifugated and the upper fluid was put to the EP tube.Plasma was separated from the blood of heart.Both plasma and BALF were kept at -80 ℃ to be determined.Results Compared with group A,arterial pressure of oxygen was significantly decreased in the group B,group C,group D(P

ObjectiveTo observe the effect of forced expansion of occlusion on radiation ankylosis of tempormandibular joint of patients with nasopharynx cancer after radiotherapy.Methods166 patients with ankylosis of tempormandibular joint were randomly divided into the treatment group (83 cases, treated with forced expansion of occlusion) and control group (83 cases, with other treatment).ResultsThe absoluteness value of degree of mouth opening in the treatment group was significantly higher than that in the control group( P<0.01), and the course of ankylosis of tempormandibular joint in the treatment group was obviously delayed compared with the control group ( P<0.01).ConclusionThe forced expansion of occlusion can delay the course of ankylosis of tempormandibular joint, expand the degree of mouth opening, recover or partially recover the occlusion and chaw function.

Adolescent ; Child ; Humans ; Insulin Resistance ; Metabolic Syndrome ; diagnosis ; prevention & control

ObjectiveTo investigate the effect of Xuebijing (Chinese herbal medicine preparation) on lung fibrosis in a rat model of oleic acid - induced lung injury.MethodsSixty SPF level of male Wistar rats were randomly ( random number) divided into three groups ( n =20 ):normal control group ( group A),oleic acid model group (group B),oleic acid and Xuebijing group (group C).Rats in group A were injected with normal saline (0.13 ml/kg) ; acute lung injury (ALI) model rats were injected with oleic acid (0. 13 ml/kg) intravenously in B and C groups.Twelve hours after administrations of saline or oleic acid into rats of three groups separately,samples of arterial blood from all rats were taken for blood gas analysis.By analysis of blood oxygen partial pressure,the acute lung injury model successfully made was confirmed.Then the rats in group C were treated with xuebijing (4 ml/kg) intravenously,once a day from the 1st to 7th day.The rats in groups A and B were treated with saline (4 ml/kg).Five rats in each group were randomly sacrificed on the 1st,7th,14th,28th day,separately.The bronchoalveolar lavage (BAL) of left lung of all rats was carried out after weight of lung weighed.The lefi lungs of rats were weighed in wet and dry in order to get the wet/dry ratio.Venous blood and BAL fluid (BALF) were obtained for detection of procollagen Ⅲ ( PC Ⅲ ) concentrations with enzyme - linked immunosorbent assay (ELISA).Right upper lobe of lung specimens was taken for histopathological examination after paraffin - embedded sections with hematoxylin-eosin (HE) staining and Masson staining.Multiple comparisons were analyzed by using oneway analysis of variance (SNK-q test).Statistical analyses were performed by using SPSS 13.0 software.ResultsOxygenation index of groups B and C ( PaO2/FiO2 ＜ 300) significantly decreased compared with the group A ( P ＜ 0.01 ).Levels of serum PC Ⅲ on the 14th day and 28th day in group C decreased significantly in comparison with group B ( P ＜ 0.05 ).Compared with group A,the levels of BALF PC Ⅲ and serum PC Ⅲ in group B and group C on the 7th day,14th day and 28th day increased ( P ＜ 0.01 ).Levels of PC Ⅲ in BALF on the 7th day,14th day and 28th day in C group decreased in comparison with group B ( P ＜0.01 ).Compared with group A,the levels of PCⅢ in BALF in group B and group C on the 7th day,14th day and 28th day increased ( P ＜ 0.05 ).ConclusionsThe result suggests that treatment with xuebijing may be useful in inhibiting pulmonary fibrosis during the course of ALI in rots.

This study was aimed to quickly and accurately identify different origins and categories of commodity edible bird’s nest (EBN) using DNA barcoding technique, in order to reveal its genetic differences. The total genomic DNA was isolated from the EBN samples. And Cytb gene sequences were amplified and sequenced by PCR. Then, 32 sequences were aligned and analyzed with DNAStar and MEGA 6.0 software. NJ phylogenetic tree was constructed. The nearest distance was calculated. The results showed that the original species of 32 samples of EBN were identified.Aerodramus fuciphagus was the genetic origin of 23 white nest samples. AndAerodramus fuciphagus germaniwas the genetic origin of the other 8 samples. The origin of black nest sample wasAerodramus maximusorAerodramus maximus lowi. It was concluded that the genetic origin of different EBN categories was variant. The identification of EBN’s origin species with Cytb sequence was quick and accurate.

Objective The research aimed to probe into the complications of the treatment that endoscopic retrograde cholangiopancreatography and endoscopic sphincterotomy (ERCP/EST) combined with laparoscopic cholecystectomy (LC) for the patients suffered from gallstone disease combined with common bile duct stones and investigate the nursing methods.Methods One hundred and twenty-four patients suffered from gallstone disease combined with common bile duct stones were included in this retrospective analysis.At first,they underwent the treatment of ERCP/EST,and underwent LC in 1 to 5 days later.All the clinical data were collected and analyzed.Results Of all the patients,104 patients successfully underwent the treatment of ERCP/EST.And of the 104 patients,100 patients successfully underwent LC,the other 4 patients underwent open cholecystectomy.Nineteen complicated with acute pancreatitises,one with hemorrhage and two suffered from infections of bile duct were observed in this study.Conclusions Combination of ERCP/EST with LC is a safe and effective treatment for gallstone disease combined with common bile duct stones.The preoperative psychological counseling,and the surveillance and nursing of complications play significant roles in the successful treatment.

Objective To investigate the status of safe injection practice in primary medical institutions.Methods 55 primary medical institutions in Hubei Province were randomly selected,questionnaire survey on status of safe injection in primary medical institutions was conducted.Results A total of 55 questionnaires were distributed,50(90.91％) valid questionnaires were obtained.All valid questionnaires were from primary level medical institutions of Hubei Province,16 of which were from community health centers,17 from township health centers,and 17 from village health clinics.None of 50 primary medical institutions repeatedly used syringe and hemostix,all used disposable sterile syringe,only 7(14.00％) medical institutions used retraction type self-destructing syringe.Only 5 (10.00％) medical institutions were equipped with hand hygiene facilities and sharps containers,32 (64.00％) medical institutions didn't provide sharps containers on treatment carts,21 (42.00 ％) injection rooms didn't install hand-drying facilities,18 (36.00 ％)medical institutions didn't provide alcohol-based hand rub on treatment carts and didn't install hand-drying facilities in treatment rooms.Of sharp injures among health care workers (HCWs) reported in 2015,114 HCWs sustained 117 times of injuries,74 cases(63.25 ％) were caused by ampoule.Of medical waste disposal,there existed some problems,such as leakage and repeated use of sharps box,didn't close sharps box timely,as well as without providing sharp containers in locations where sharps might be produced.Conclusion There are a variety of hidden risks of unsafe injection in primary medical institutions,like insufficient facilities and unsafe disposal of medical waste.