Objective To study the diagnostic value of pencilliosis marneffei (PM)in a non-HIV-infected child with the com-bined detection of aspergillosis galactomannan,fungus Glucan(1-3)-β-D and boold culuture.Methods The venous blood specimen from the child was collected for the quantified detection of aspergillosis galactomannan,fungus Glucan(1-3)-β-D. The growth and colonial morphology of fungus was inspected with the positive blood culture and the characteristics of fun-gus smear were observed under microscope.Results The result of aspergillosis galactomannan was 14.45 μg/L and fungus Glucan (1-3)-β-D 77.14 pg/ml.Penicillium marnrffei was identified using blood culture.It was mycelia form under 25℃ and the salouraud medium produced water soluble claret-red pigment produced.It was mycelia form under 35℃ and the colony was gyri creases,the characteristic broom-like hypha and separation hypha could be found under microscope.Conclusion It is effective for the early diagnosis and therapy of PM with the combination detection of aspergillosis galactomannan,fungus Glucan (1-3)-β-D and boold culuture and have better clinical diagnosis value.

Hepatitis B ; immunology ; prevention & control ; Hepatitis B Vaccines ; immunology ; Humans ; Immunization Schedule ; Vaccination ; Vaccines, Synthetic ; immunology

Objective To evaluate the clinical effect of Tanreqing in the treatment of radiation pneumonia. Methods 75 patients with radiation pneumonia were randomly divided into treatment group and control group according to the registration of this study.38 cases in the treatment group treated by Tanreqing,antibiotics and glucocorticoid,while 37 cases in the control group was given antibiotics and glucocorticoid.Then,the therapeutic efficacy was compared.Results From the chest routine scan result and the clinical symptom release,the excellence rate of the treatment group was 68.4%,which of the control group was 43.2%,there was statistically significant difference between the two groups (χ2 =4.823,P =0.028 ).Conclusion Treating radiation pneumonia with Tanreqing has good effect,and it is worthy of clinical application.

According to morphological and microscopic characteristics, a high-efficient decolorizing fungus, the strain Asaw117, was identified as Aspergillus awamori. Selecting eight different dyes from Azo dyes, anthraquinones dyes and oxygen Quinones dyes, the decolorizing assays of various dyes showed that the strain Asaw 117 was the highest decolorizing potential to 0.1 g/L Vat Blue RSN, the discoloration rate up to 100 percent. Comparing to different kinds of medium and several of carbon and nitrogen sources, the strain had the best decolorizing efficient although grew slower in the Czapek medium, otherwise, grew quicker and decolorizing efficient lower in the PDF medium. It could use Vat Blue RSN as a nitrogen source, but not as a carbon source. The medium composing of saccharose and ammonium nitrate as carbon and nitrogen sources was decolorizing potential markedly during different combinations of carbon and nitrogen sources. So the strain has good potential for the dyeing wastewater treatment

OBJECTIVETo explore clinical significance of rotational axis of distal femur on Chinese adults in total knee arthroplasty (TKA).

METHODSThere were 86 Chinese adults (106 normal knees) including 47 males (53 knees) and 39 females (53 knees), 54 knees were on left and 52 on right. The CT scan was employed in the distal femur. The scan direction was aligned to be on the plane perpendicular to the mechanical axis of the femoral. The CT images of cross sections across lateral and medial femoral epicondyle were moved to personal computer,lateral angle between anterior posterior line (APL) and surgical transepicondylar axis (STEA) (ATA),lateral angle between posterior condylar line (PCL) and APL (APA), angle between perpendicularity of APL and PCL (A-PA), posterior condylar angle (PCA), condylar twist angle (CTA), angle between clinical transepicondylar axis (CTEA) and STEA (CSA) were measured. These values were divided into different groups according to gender and side, the values of CTA, PCA, A-PA, angle PT (varus of tibia plateau), constant 3, ATA, APA and constant 90° were compared by statistically. A-PA and PCA, and CTA were analysed statistically with the liner regression, the relationship among CTEA, STEA ,PCL, APL and PLP were performed to assess by liner regression.

RESULTSATA was (89.79 ± 1.22)°, APA was (84.84 ± 1.83)°, A-PA was (5.16 ± 1.83)°, PCA was (4.80 ± 1.23)°, CTA was (8.23 ± 1.40), CSA was (3.45 ± 0.68)°. All the parameters had no differences on sex and side,but CSA had difference on male and female. There was no difference among angle PT, PCA, A-PA. There was significant difference in CAT, constant 30 and angle PT, PCA,A-PA. There was no difference between ATA and constant 90°, but there was difference between APA and constant 90°. There was relativity between PCA and CTA, and also PCA and A-PA, CTA and A-PA. There was significant relativity between STEA and CTEA, between STEA and APL, between STEA and PCL, and also between APLP, APL and PCL, but there was no significant relativity between PCL and CTEA.

CONCLUSIONTKA for Chinese, the section of femoral posterior condyle should be external 5° to obtain the optimum rotational orientation. The property is different entirely between STEA and CTEA, the rotational alignment is not performed according to parallel to the CTEA in distal femur. Among STEA, APL, PCL, the STEA is the most reliable mark ofrotational alignment of the distal femur, and the PCL is the less reliable mark.

Adult ; Arthroplasty, Replacement, Knee ; Female ; Femur ; anatomy & histology ; diagnostic imaging ; Humans ; Male ; Middle Aged ; Rotation ; Tomography, X-Ray Computed

Objective To determine the clinical efficacy of limited internal fixation with posterior titanium plates for unstable atlas fractures.Methods A retrospective case series analysis was performed for 29 patients with unstable atlas fractures immobilized using the posterior titanium plate from January 2011 to November 2014.There were 22 males and seven females,with a mean age of 41.5 years (range,17-68 years).Ten patients were diagnosed with 1/2 ring Jefferson fractures,12 anterior 1/2 Jefferson fractures and seven patients posterior 3/4 Jefferson fractures.Frankel neurological performance scale was grade E in all patients.Operation time,amount of bleeding during operation and postoperative complications were recorded.X-ray and CT were performed to identify the fusion,internal fixation location and atlantoaxial stability.Visual Analogue Scale (VAS) for craniocervical pain and cervical range of motion were evaluated.Results Mean duration of procedure was (86.6 ± 7.8) min (range,68-122 min) and mean blood loss was (120.0 ± 25.3) ml (range,90-400 ml).No vascular and neurologic complications were noted.All patients were followed up for (22.5 ± 18.0)months (range,12-32 months).CT scans confirmed bony union in all patients 6 months after operation.No plate-related complications,implant lessening and implant breakage occurred during the follow-up.Postoperative VAS [(1.9 ± 1.1) points] was significantly lower than that before operation [(7.2 ± 1.4) points] (P ＜ 0.05).At the last follow-up,the ranges of cervical flexion-extension [(81.3 ± 22.2) °] and rotation [(152.4 ±38.7) °] were close to the normal range.Conclusion Limited internal fixation with posterior titanium plates is a safe and effective surgical method that contributes to good reduction and maximal preservation of cervical physiological motion.

Objective To observe the effect of dihydromyricetin of ampelopsis grossedentata on lipid metabolism and antioxidation in atherosclerosis (AS) rats. Methods Sixty Wistar rats were randomly divided into five groups (n = 12 each):normal control group, model control group, positive control group, high dose dihydromyricetin group (40 mg·kg-1 ) and low dose dihydromyricetin group (10 mg·kg-1 ). Except normal control group, rats in the other groups were injected with a single dose of vitamin D3(600 000 U·kg-1 ) and loaded with high fat diet to establish AS model. Simvastatin (5 mg·kg-1 ) was intragastrically administered to positive control group. High and low dose dihydromyricetin groups received intragastric administration of 40 and 10 mg·kg-1 dihydromyricetin, respectively. Equal volume of purified water was given to normal and model group. After 24 weeks of administration, serum levels of lipids, activities of superoxide-dismutase (SOD) and malonicdialdehyde (MAD) were determined. Results As compared with model control group, triglycerides (TG) of high and low dose dihydromyricetin groups was decreased [(191. 65±101. 10) vs. (111. 10±29. 29) and (120. 55±38. 12) mg·L-1 , respectively], total cholesterol (TC) was decreased [(151. 64±33. 62) vs. (148. 49±30. 14) and (118. 90±27. 38) mg·L-1 ], and high density lipoprotein cholesterol (HDL-C) was increased [(1. 29±0. 68) vs. (2. 10±0. 70) and (1. 62±0. 61) mmol·L-1 ], low density lipoprotein cholesterol (LDL-C) was decreased [(5. 01±1. 33) vs. (3. 97±0. 78) and (4. 28±0. 79) mmol·L-1 ], activity of SOD was increased [(141. 03±42. 52) vs. (187. 97±42. 08) and (150. 99±46. 17) U·mL-1 ], and MDA was decreased [(20. 51±3. 81) vs. (17. 64±1. 54) and (18. 52± 3. 42) nmol·mL-1 ], with significant differences (P<0. 05, P<0. 01). Conclusion Dihydromyricetin can reduce the level of serum lipid, improve antioxidation activity, and has therapeutic effect for atherosclerosis.

Objective To investigate the incidence and treatment of posttraumatic cauda equina herniation associated with lumbar fracture.Methods A retrospective analysis was performed on 416 patients admitted due to lumbar burst fracture between June 1,2008 and June 1,2011.All the patients had been operated on via anterior or posterior approaches.Data collected included demographics,injury mechanism,radiographs,surgical procedures,outcomes and follow-up.Incidence of cauda equina herniation was documented.Results All surgeries were uneventful.Cauda equina herniation was observed in 49 patients (11.8％) during surgical exploration.Anterior surgery was done on 115 patients and found 7.8％ incidence of cauda equina herniation (9/115).Posterior surgery was done on 301 patients and found 13.3％ incidence of cauda equina herniation (40/301).There were 40 patients combined with laminar fracture and 38 of them had cauda equina herniation with the incidence rate of 95％.Among the 49 patients with cauda equina herniation,44 (90％) had neurological deficit and 5(10％) were found with good neurological function but significant proportion of cauda equina rootlets entrapped in the lamina fracture.Conclusions Lumbar burst fracture combined with neurological deficit and laminar fracture may increase the risk of posttraumatic cauda equina herniation and should be paid attention.Operations should be performed with caution to avoid further nerve damage.

Objective To investigate the epidemic situation of coal-burning-fluorosis,and to explore key areas for implementing prevention and control measures in Guizhou Province.Methods Survey data of dental fluorosis of children aged 8-10,anti-fluoride knowledge and health-related behaviors of target students and their families before implementing the comprehensive disease control measures in 2010,were analyzed and evaluated using the method of factor analysis in 23 diseased districts.Results ①The three main factors (F) related to the endemic of coal-burning-fluorosis were economic factor (F 1),knowledge factor (F2) and behavior factor (F3):F1 included the detection rate of 8 to 12 years old children's dental fluorosis,iron stove ownership,corn correct drying rate and chili correct drying rate; F2 included stove correct utilization rate,students awareness and head of the household awareness; F3 included corn food washing rate before cooking and chili food washing rate.②In the economic factor score arrangement,the first three were Zunyi,Xiuwen and Qingzhen.In the F2 score arrangement,the first three were Zunyi,Xishui and Xixiu.In the F3 score arrangement,the first three was Liuzhi,Bijie and Xixiu.③After comprehensive score,Zunyi,Qingzhen,Xishui,Xixiu,Puding and Liuzhi Counties were found to be relatively stronger in control of the epidemic of coal-burning-fluorosis; Xiuwen,Renhuai,Guanling and so on 13 counties were found to be relatively moderate,and Tongzi,Hezhang,Shuicheng and Nayong Counties were found to be relatively weak.Conclusions ①The endemic of coal-burning-fluorosis is related with many factors,and the indexes we selected in this study have a strong correlation and sensitivity,which may be used in evaluating the prevention effect of control measures to coal-buming-fluorosis.②The endemic of coal-burning-fluorosis in Guizhou Province is quite different,and key prevention and control measures can be applied according to a comprehensive factor analysis.

Objective To synthesize and fusion express the predicted T-cell epitopes of Schistosoma japonicum, and analyze their immunogenicities. Methods The plus and minus oligo-nucleic acid strands of epitopes P7, P17, P18 were synthesized following their DNA sequence, respectively. The Nco I restriction enzyme sites were added to the 5′ end of epitope gene and the Xho I restriction enzyme sites were added to the 3′ end of epitope gene. The plus and minus strand of each epitope gene was annealed to form double strand DNA fragments. Then the double strand DNA fragments encoding epitope peptide were cloned into the site between Nco I and Xho I of plasmid pET32c(+) to construct recombinant plasmid which was transformed into E.coli DH5?. The recombinant plasmid containing P7, P17, P18 genes respectively was identified by PCR, restriction digestion and DNA sequencing, and then transformed into E.coli BL21 (DE3) for expressing the fusion protein. The fusion protein of peptide-thioredoxin(Trx) was expressed by inducing with IPTG and analyzed with SDS-PAGE. The fusion proteins were purified with Ni2+ column affinity chromatography. Meanwhile, the peptides P7, P17, P18 were synthesized artificially following their amino acid se-quence. By using the purified epitope peptide fusion proteins and synthesized epitope peptides, the splenic cells of C57BL/6J mice immunized with ultraviolet-attenuated cercaria of Schistosoma japonicum were stimulated respectively. The stimulation activity of fusion proteins and synthesized peptides were assayed by detecting the incorporation rate of 3 H-thymidine. Results The double strand DNA fragments of epitopes P7, P17, P18 were successfully cloned to form recombinant plasmids, all of which could express a 20 kDa fusion protein. Both the fusion protein and synthesized epitope peptides of P7 and P17 were able to stimulate the lymphocyte cells to proliferation effectively. Conclusion The peptide P7 and peptide P17 are testified as T-cell epitopes of Schistosoma japonicum.