Objective:To evaluate the in vitro cytotoxicity,in vivo response and degradation of a new injectable nano-hydroxyapatite(n-HA)/calcium sulphate hemihydrate(CSH) bone substitute in rabbits and to discuss its possible bio-degeneration mechanism.Methods: Pure CSH,n-HA,10%n-HA+CSH,20%n-HA+CSH,and 40%n-HA+CSH(the last 3 containing 10%,20%,and 40% n-HA,respectively) were subjected to MTT assay for their in vitro cytotoxicities.20%n-HA+CSH was implanted into rabbits' muscle and bone defects in the right femur,and the general conditions of rabbits,the pathological and TEM manifestations of tissues,and the imaging alterations were all observed 5 days,2,3,4,6,8,and 12 weeks after implantation.Results: Test of cytotoxicity showed that the average cell proliferation rate was over 77% after treated with the solutions of 5 bone substitutes(P

Regorafenib,an oral multi-kinase inhibitor,can inhabit a class of receptor tyrosine kinase,such as angiogenic,stromal,oncogenic and so on.Studies in vitro and clinical trials indicate that Regorafenib has significant antitumor activity.The results of clinical trials are encouraging for the treatment of Refractory solid tumors,especially for colorectal carcinoma.

Objective: To evaluate the in vitro cytotoxicity, in vivo response and degradation of a new injectable nanohydroxyapatite(n-HA)/calcium sulphate hemihydrate(CSH) bone substitute in rabbits and to discuss its possible bio-degeneration mechanism. Methods: Pure CSH, n-HA, 10%n-HA+CSH, 20%n-HA+CSH, and 40%n-HA+CSH (the last 3 containing 10%, 20%, and 40% n-HA, respectively) were subjected to MTT assay for their in vitro cytotoxicities. 20%n-HA+CSH was implanted into rabbits' muscle and bone defects in the right femur, and the general conditions of rabbits, the pathological and TEM manifestations of tissues, and the imaging alterations were all observed 5 days, 2, 3, 4, 6, 8, and 12 weeks after implantation. Results: Test of cytotoxicity showed that the average cell proliferation rate was over 77% after treated with the solutions of 5 bone substitutes(P<0.05), with the cytotoxicity being 0-1 (non-toxic). No general reaction was found in rabbits implanted with 20%n-HA+CSH and the weight of animals increased steadily. 20%n-HA+CSH implanted in the muscle underwent a layer-by-layer degradation from 5 days after implantation and was almost completely degraded after 8 weeks, with no fibrosis, calcification or ectopic ossification. H-E staining showed that at the early stage the degraded materials were surrounded and wrapped by inflammatory cells; the fibroblasts gradually turned into fibrous cells encircling the degraded material. TEM observations indicated that the degradation was characterized by a cytophagic process and no cytotoxic signs were found in the phagocytes after phagocytosis. X-ray examination showed new osteoid and premature trabeculae scattered over the defective region 6 weeks after implantation, and there was normal trabecular tissue 8-12 weeks later. Conclusion: 20%n-HA+CSH has no obvious in vitro cytotoxicity, but has good biocompatibility. The in vivo degradation of 20%n-HA+CSH may be mediated by cytophagic process in a layer-by-layer manner. It degrades completely within 8-12 weeks, which is suitable for bone regeneration.

Objective To study the cause of occurrence and development in senile DN,to provide ready and effective ways of treatment and precaution.Methods Case history information of DN and NDN patients over 60 years was selected randomly;The questionnaiere was given to the patients;Their blood pressure,blood sugar,blood-fat,urinary albumin and renal function were monitored and conducted comparative analysis.Results There was a long course of disease,bad control of blood sugar,higher than 10mmol/L exceeding 2 5years and accompanying disorder of blood-fat and then gradual renal insufficiency and other multi-complication in senile DN patients.Conclusion Longtime hyperlycemia,hyperlipemia were important risk factors.Micro-albumin urinary indicates different degree of damage of kidney.Good control of blood sugar can decrease and delay occurrence and development of senile DN.

Objective To observe the clinical effect of Angelica injection combine with psychological intervention in treatment of patients with lower limb fracture postoperative analgesia.Methods 60 cases underwent lower limb fracture surgery from February 2013 to April 2015, and were randomly divided into experimental group and control group, 30 cases in each group.Control group were treated with conventional medicine epidural injection morphine for postoperative analgesia, experimental groups were injected with Angelica injection treatment in control group on the basis of conventional therapy, recorded and analyzed two groups of patients improved postoperative pain scores, postoperative stress and pain after treatment. Results After treatment, 4, 8, 24, 48h pain scores of experimental group were lower than of control group (P<0.05); After 4h, 48h post-operation heart rate and mean arterial blood pressure was better than of control group (P<0.05).Conclusion Effect of conventional western medicine combined with Angelica injection postoperative analgesia is superior to conventional medicine alone , with no obvious side effects.

Histocytological Preparation Techniques ; Humans ; Immunohistochemistry ; Microscopy, Electron ; methods ; Molecular Biology ; methods ; Staining and Labeling

Objective To observe the clinical efficacy of integrated traditional Chinese and western medicine in the treatment of infertility resulted from ovulation dysfunction.Methods 106 patients with ovulation dysfunction were divided into observation group(n =56)and control group(n =50)in stratified randomization.Both two groups were given clomiphine to induce ovulation.Futhermore,the observation group was added Chinese herb recipe.Both two groups were treated for 3 menstruations.The basic body temperature,follicle development,endometrium depth, ovulation and pregnancy were observed.The serum levels of E2,LH and FSH were detected.Results There was no difference in the basic body temperature between the two groups(P >0.05).After treatment,the basic body tempera-ture of the observation group was obviously superior to that of the control group(χ2 =0.627,P =0.049),there was no difference in the endometrium depth between the two groups(P >0.05).After treatment,the endometrium in the observation group was significantly better than before treatment and that in the control group(t =5.132,P <0.05). After treatment,the serum levels of E2 ,LH and FSH in the observation group were all improved more obviously than those in the control group(t =6.489,5.167,6.035,all P <0.05).The efficacy of the two groups was higher than that of the control group(χ2 =6.049,P =0.048).Conclusion The method of integrated traditional Chinese and western medicine can promote the development of follicle and endometrium,improve levels of E2,LH and FSH,enhance ovula-tion rate and pregnancy rate in the treatment of infertility resulted from ovulation dysfunction,which is worthy of clini-cal promoting.

The authors have studied the changes in the concentration of glutathione of the red blood cell (RBC-GSH) and plasma malondialdehyde (MDA) in 54 patients with acute brain stroke. The results indicated that the concentration of RBC-GSH reduced obviously and that of plasma MDA increased markedly within 72 hours after illness. However, there is no obvious difference between the cases of hemorrhagic cerebrovascular diseases and cerebral infarction group. The RBC-GSH level rised again in the 7th day, while the plasma MDA still remained higher level, they were normal during the convalescent period. The pathological basis of the change may be the response of the free radicals in human body.

[Objective]To compare the result of treatment with double bands of Atlas Ti-cable and clavicular hook plate for total acromioclavicular dislocation.[Method]Fifty-two total acromioclavicular dislocation were treated with two different fixation.1)by double bands of Atlas cable(DC group,28 cases) and 2)clavicular hook plate(HP group,24 cases).There were 38 males and 14 females,39 acute and 13 chronic,all unilateral.The mean age of patients was 38.5 years(22~65).A sling of double cable was fixed through the base of the coracoid process and the clavicle at 1.5 cm upper medially above coracoid process.All hook plate were 6 holes.Coracoclavicular ligament was reconstructed routinely.[Result]All patients were available for an average duration of follow-up of 18.8 months(range,6~42 months).The results were evaluated by radiographic measurement and clinical recovery(muscle strength in upper arms,pain in shoulder,range of joint motion) by Lazcano standard.In HP group,14 patients were assessed as good,6 as fair and 4 as poor(good and fair 83%).In DC group,22 patients were assessed as good,5 as fair and 1 as poor(good and fair 96%).There was no statistical difference between the two groups.[Conclusion]In the treatment of acromioclavicular dislocation,fixation of double bands of Atlas cable is believed to be as good as hook plate fixation.Meanwhile,Atlas cable is featured with less pain followed operation,which in favor of early exercise.For it's good biocompatibility,titanium cable can be retained in body and does not need to be removed.