Aim:To develop a method for rapid and sensitive simultaneous determination of the hydrophilic and lipophilic active components in Salvia miltiorrhiza tablets. Methods:RP-HPLC condition was established to separate danshensu,salvianolic acid B,cryptotanshinone and tanshinone ⅡA from each other on the Diamonsil C18(250 mm×4.6 mm ID,5 μm)column with the mobile phase consisted of methanol and 0.5% formic acid in gradient elution. Results:The four active components had good linearity. The average recoveries ranged between 96.63% to 100.3% with RSDs of less than 2%. Conclusion:The method appeared to be precise and accurate for the simultaneous determination of the four active components in Salvia miltiorrhiza Bunge tablets within 35 min.

By researching the relevant papers at home and abroad, the principles of preparation, drug release mechanism, existing problems and so on were analysed and summarized on prodrugs triggered by colon bacteria-releasing enzymes (PTCBE). Compared with ordinary drugs, PTCBE was degraded by colon bacteria-releasing enzymes on colon-targeted treatment. PTCBE have the drug release characters of specificity, accuracy and so on for the colon-targeted treatment and can reduce the adverse drug reactions. Thus PTCBE have the good prospects of applications.

Objective To summerize the experience in diagnosis and surgical treatment for hilar cholangiocarcinoma in recent years.Methods 86 consecutive cases of hilar cholangiocarcinoma were surgically treated in this hospital from Jan. 1992 to Oct. 1998. Results The tumor resection rate was 37%(32/86) in this series. The postoperative morbidity and mortality rate were 47% and 7% respectively. The median survival time in palliative resection group was 16 months and the 1-year, 3- year, 5-year survival rate was 63%,21%,15% respectively. For curative resection,the median survival time was 19 months and the 1-year, 3- year, 5-year survival rate was 80%,35%,25% respectively( P =0 038). For drainage group, the median survival time was 5 months and the 1-year, 3- year, 5-year survival rate was 28%,8 4%,5% respectively.Conclusion Early diagnosis, improvement of patients′ general preoperative condition and the surgical expertise may help in increasing the curative resection rate and reducing postoperative morbidity and mortality rate, which are keys to prolong patients′ survival.

AIM: To elucidate the effects of platelet-activating factor (PAF) on eosinophil activation and the action of dexamethasone or theophylline during this process. METHODS: Eosinophils (EOS) from the peripheral blood of normal subjects were isolated. The hypodense eosinopil (HE) and normodense eosinophil (NE) were studied with electron microscopy. The effects of PAF on eosinophil activation and the action of dexamethasone or theophylline during the above process were measured. RESULTS: Hypodense eosinophil had significantly smaller individual granules than normodense eosinophil had. PAF induced eosinophil peroxidase release, and generated. Eosinophils incubated with 10~(-8) mmol/L PAF and 10~(-5) mmol/L dexamethasone released (101.17?10.32) mg/L eosinophil peroxidase (P

Objective To evaluate the efficacy and safety of Liqifuwei oral liquid for prophylaxis of chemotherapy induced-constipation through a randomized controlled trial.Methods Cancer patients who met the inclusion criterias were randomly assigned to the treatment group and the control group by random number table.Liqifuwei oral liquid was prescribed 20 ml per tid from the first day of chemotherapy in the treatment group,the control group accepted the conventional treatment.Incidence and degree of chemotherapy inducedconstipation,degree and duration time of abdominal distension,anorexia and diarrhea were compared by criteria of NCI-CTC 3.0.Results A total of 100 patients were eligible,including 50 patients in the treatment group and 50 patients in the control group respectively.Incidence rate of chemotherapy induced-constipation was 48 ％ (24/50) and 98 ％ (49/50) (P ＜ 0.000 1),all patients were grade 1-3 constipation,no grade 4 and grade 5 constipation (P =0.036).Incidence rate of abdominal distension was 36 ％ (18/50) and 70 ％ (35/50)(P =0.001 3),abdominal distension degree (P =0.033),duration time of abdominal distension was (2.22±1.48)days and (3.43±1.60) days (P =0.01).Incidence rate of anorexia was 56 ％ (28/50) and 90 ％ (45/50)(P ＜ 0.000 1),there was no significance different of anorexia degree (P =0.818).One patient in treatment group underwent mild diarrhea.Conclusion The prophylaxis of Liqifuwei oral liquid for chemotherapy induced-constipation and abdominal distension is effective,which deserves large-sample studies and clinic application.

BACKGROUND:Until now, musculoskeletal bioengineering used in the orthopedic surgery and regenerative medicine are developing. OBJECTIVE:To summarize the construction strategies for tissue-engineered knee ligaments. METHODS:A computer-based online search was conducted in PubMed to screen the relevant literatures using the terms“knee ligament”AND“tissue engineering”OR“regenerative medicine”. The literature retrieval, data assessment and extraction were performed by both authors independently. The relevant literatures were included through reviewing preclinical and clinical studies to display the preclinical and clinical medicine progresses in the construction strategies for tissue-engineered ligaments. RESULTS AND CONCLUSION:Included 224 literatures focus on the construction strategies for tissue-engineered ligaments, including stem cel therapy, growth factors, biomaterials and scaffolds, and mechanical stimulation pattern. Construction techniques for tissue-engineered ligaments have some potential advantages, such as rapid function recovery, good tenacity, and reduced relapse. However, rigorous and high-level randomized control ed clinical trials are needed for the translation of preclinical results into successful clinical trials.

Blood samples were detected by 24 blood-glucose meters using glucose oxidase and another 18 glucose meters using glucose dehydrogenase. The plasma glucose was detected by the auto-chemistry analyzer as control. According to ISO1 5197 and EF9-A2, the bias of results from both glucose meters 100% fall in the range of ±0. 56 mmol/L( ＜4. 2 mmol/L) and ≥99. 7% in the range of ±20% ( ≥4. 2 mmol/L), predicted bias were all less than the true bias(Bc). As κ＞0. 6, the results from both glucose meters were in accordance with the results from autochemistry analyzer in judging hyperglycemia ( ＞6. 11 mmol/L) and hypoglycemia ( ＜ 3.89 mmol/L). Between the results from the two blood-glucose meters, κ＜0. 6. The accuracy of both glucose-meters are accepted for the purpose of clinical diagnosis and treatment.

Objective To explore the molecular mechanism through detecting methylation status in esophageal carcinoma and Barrett's esophagus.Methods The methylation-specific PCR was employed to detect the expressions of RASSF2 in esophageal carcinoma tissues,adjacent tissues from 27 patients after surgery and Barrett's esophagus tissues from 18 patients after gastroscopic biopsy.Results The rate of RASSF2 methylation was much higher in esophageal carcinoma (66.7 ％,18/27) than that in adjacent tissues (22.2 ％,6/27) (P ＜ 0.05),and was more frequently than that in Barrett's esophagus (33.3 ％,6/18) (P ＜ 0.05).However,no significant difference of methylation status was observed between Barrett's esophagus and adjacent tissue (P ＞ 0.05).Conclusion The hypermethylation of RASSF2 may be the molecular mechanism in the development of esophageal carcinoma,and more samples should be used for further experiment in Barrett' s esophagus.

Objective To evaluate the application of 64-slice computed tomography (CT) and software-assisted image analysis in preoperative evaluation and surgical planning of hilar cholangiocarcinoma. Methods The clinical data of six patients with hilar cholangiocarcinoma who were admitted to The First Affiliated Hospital of Sun Yat-sen University from June to December, 2009 were retrospectively analyzed. All six patients underwent 64-slice CT scanning before the operation, and then three-dimensional (3D) reconstruction of the liver, tumor, blood vessels and bile ducts was achieved with the assistance of Myrian (R) XP-Hepatic software. The relationships of the tumor, blood vessels and bile ducts were illustrated in the 3D model. Virtual liver resection was carried out for surgical planning. Results The 3D models of the liver, tumor, blood vessels and bile ducts were successfully constructed . The 3D models were able to be rotated and magnified as necessary. Preoperative evaluation and surgical planning were in accordance with actual surgery. The relative accuracy of the software-assisted image analysis system for calculating the hepatic volume to be resected was 5%. Patients recovered well without occurrence of hepatic failure. Conclusion Sixty-four-slice CT scanning and software-assisted image analysis are important for preoperative evaluation and surgical planning of hilar cholangiocarcinoma.

Objective To detect the expression pattern of microRNA in A549 cells treated by lipopolysaccharide, study the expression of miRNA-1247-3P in A549 cells under LPS treatment and explore the possible mechanism of miRNA-1247-3P in A549 cells under LPS treatment. Methods A549 cells were divided into experimental and control groups. Immunocytochemical method and RT-PCR were used to detect the changes of SP-A and SP-C. The expression of miRNAs were detected using miRNAs array in different groups. The key miR-1247-3P was collected to detect the changes of miR-1247-3P in all groups using quantitative real-time polymerase chain reaction. Results Compared with control group, the expressions of SP-A and SP-C were significantly decreased in the experimental groups (P < 0.05). MiRNA array showed that 31 miRNAs were up-regulated and 3 miRNAs were down-regulated. Compared with control group, the expression of miR-1247-3P was significantly increased in the experimental groups (P < 0.05). Conclusion The increased expression of miR-1247-3P may play an important role in the pathogenesis of ARDS.